Abstract
To block and weaken the bacterial branched VB12 synthetic metabolic pathway, homologous recombination technology was used to knock out the sirohaem synthase gene cysG located in the chromosome and the endogenous A plasmid of the Ensifer adhaerens Casida A strain, and the expression of the uroporphyrinogen III decarboxylase gene hemE was weakened by weak promoter substitution. The growth of the engineered strains and the production of VB12 and haem were analysed and measured in the engineered strains, aiming to provide a new strategy for enhancement of VB12 biosynthesis. The results showed that the chromosomal cysG gene knockout strain ΔcysG, endogenous A plasmid cysG gene knockout strain ΔpAcysG and cysG gene double knockout strain ΔcysGΔpAcysG grew normally, with VB12 yield increases of 19.9%, 11.2%, and 27.4% compared to the starting strain, respectively. In the background of the cysG gene knockout strain, the expression of the hemE gene was weakened, resulting in the generation of the strain ΔcysGΔpAcysG-E-pdnaD, and the VB12 yield of ΔcysGΔpA cysG-E-pdnaD reached 114.17 ± 5.77 mg L−1, an increase of 45.1% compared to the yield of the original strain. The above results indicate that the strategy of increasing VB12 production by knocking out the haem synthesis pathway and weakening the haem synthesis pathway is effective.
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The datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request.
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The authors thank AJE for providing polishing services for this article.
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This work was supported by the Key Research and Development Program of Ningxia 2021BEG02003.
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YHL, YYC and JYS contributed to the conception and design of the researches and the interpretation of the data. YHL and YYC performed the experiments. YHL, YYC and QW performed some strains construction and VB12 detection. CLM and WH performed strains cultivation. YHL, QW and WH contributed to manuscript writing and figures making. All authors critically viewed, edited and approved the manuscript. All authors read and approved the final manuscript. YHL and YYC contributed equally to this work.
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Liu, Y., Chang, Y., Wang, Q. et al. Effect of blocking the haem synthesis pathway and weakening the haem synthesis pathway for sirohaem on the growth of and vitamin B12 synthesis in Ensifer adhaerens Casida A. Bioprocess Biosyst Eng 46, 1825–1835 (2023). https://doi.org/10.1007/s00449-023-02939-5
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DOI: https://doi.org/10.1007/s00449-023-02939-5