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Development of tissue culture system with automated pulsation and Kalman filter control for an artificial artery model

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Abstract

Tissue-engineered arterial vessels have been used as substitutes for unnecessary animal experiments to evaluate the pharmacokinetics of drugs targeting various arteriopathies caused by structural or physiological arterial defects. An arterial tissue culture system was established to simulate the mechanical characteristics of a heart-beating pump and to do online feedback control of lactate and glucose concentrations. The mechanically controlled flow pump mimicked the heart pumping inside a tissue-engineered artery composed of muscle and endothelial cells within a nanofibrous scaffold. After monitoring the pH of the culture medium online, lactate and glucose were estimated using the Kalman filter algorithm, and the set-point online control was operated to maintain glucose for artery tissue engineering. The composition of the artificial artery was confirmed by immunofluorescence staining, and its mechanical characteristics were examined. The online automated system successfully demonstrated its applicability as a standardized process for arterial tissue culture to replace animal arterial experiments.

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Data availability

The data are not openly available due to sensitivity and are available from the corresponding author upon reasonable request. Data are stored at the lab of the corresponding author.

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Acknowledgements

This work was supported by Inha University.

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  1. Sang-Myung Jung and Byung Man Lee have contributed equally to this work.

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  1. Department of Biological Engineering, Inha University, Incheon, 22201, Republic of Korea

    Sang-Myung Jung, Byung Man Lee & Hwa Sung Shin

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  1. Sang-Myung Jung
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Jung, SM., Lee, B.M. & Shin, H.S. Development of tissue culture system with automated pulsation and Kalman filter control for an artificial artery model. Bioprocess Biosyst Eng 46, 1437–1446 (2023). https://doi.org/10.1007/s00449-023-02910-4

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