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Investigation of in vitro antimicrobial, antioxidant and antiproliferative activities of Nostoc calcicola biosynthesized gold nanoparticles

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Abstract

The cyanobacteria are the promising candidate for synthesizing gold nanoparticles (AuNPs), due to their ability to accumulate heavy metals from the cellular environment and additionally contain varied bioactive compounds as reducing and stabilizing agents. This study describes the N2-fixing cyanobacterium Nostoc calcicola-mediated bioreduction of AuNPs and the inherent antimicrobial, antioxidant, and antiproliferative activities in vitro. Biosynthesized Nc-AuNPs were characterized by spectral characterization techniques. The formation of AuNPs was physically confirmed by the colour change from pale green to dark violet. The UV–Vis analysis, further, proved the reduction in Nc-AuNPs with the cyanobacterium and showed a spectral peak at 527 nm. FESEM-EDX images suggested the surface morphology of the NPs as spherical, cuboidal, and size between 20 and 140 nm. The antimicrobial studies of Nc-AuNPs were carried out by agar-well diffusion method and MIC values against five pathogenic bacterial and two fungal strains were noted. The AuNPs exhibited potential antimicrobial activity against h-pathogenic bacteria with inhibitory zones ranging at 11–18 mm; against fungi ranging at 13–17 mm. Significant antioxidant potentialities were explored by a DPPH assay with an IC50 value of 55.97 μg/ mL. Furthermore, in the anticancer efficacy assay, the Nc-AuNPs inhibited cellular proliferation in human breast adenocarcinoma and cervical cancer cell lines at IC50 concentration, 37.3 μg/ml, and 44.5 μg/ml, respectively. Conclusively, N. calcicola would be an excellent source for synthesizing stable colloidal AuNPs that had significant credibility as phycological (algal) nanomedicines as novel prodrugs with multiple bioactivities.

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Acknowledgements

The authors are grateful to Dean IMS & SUM Hospital, Siksha O Anusandhan, deemed to be University (SOADU), Bhubaneswar-30 for necessary facilities. This is a piece of work supported by SOADU, PhD-Fellowship No.1981611007/2019. We are grateful to the honorable VC of SOADU, Prof. Dr. PK Nanda for encouragements.

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  1. Chita Ranjan Sahoo

    Present address: ICMR‐Regional Medical Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Govt. of India, Bhubaneswar, India

Authors and Affiliations

  1. Central Research Laboratory, Institute of Medical Sciences, & Sum Hospital, Siksha O Anusandhan Deemed to be University, Bhubaneswar, Odisha, 751003, India

    Chinmayee Priyadarsani Mandhata, Ajit Kumar Bishoyi, Chita Ranjan Sahoo, Surendra Swain, Shuvasree Bej & Rabindra Nath Padhy

  2. Department of Chemistry, Veer Surendra Sai University of Technology, Burla, Sambalpur, Odisha, 768018, India

    Bigyan Ranjan Jali

  3. Kode Lab, Tumor Immunology and Immunotherapy Group ACTREC, Tata Memorial Centre, Kharghar, Navi Mumbai, 410210, India

    Rajesh Kumar Meher

  4. Medical Research Laboratory, IMS and SUM Hospital, Siksha O Anusandhan Deemed to be University, Bhubaneswar, Odisha, 751003, India

    Debasmita Dubey

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Contributions

CPM: contributed to conceptualization, Investigation, Methodology, Data curation, formal analysis, writing-original draft, and editing. AKB: contributed to conceptualization, Methodology, Validation; Visualization, Writing, review, editing, CRS: contributed to conceptualization, Validation, Methodology, SS: contributed to formal analysis, SB: contributed to formal analysis, BRJ: contributed to methodology, validation, RKM: contributed to antiproliferative activity Methodology, Writing and editing, DD: contributed to antiproliferative activity, Reviewing, Editing. RNP: contributed to conceptualization, Supervision, Editing, Validation, and fund acquisition.

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Correspondence to Rabindra Nath Padhy.

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Mandhata, C.P., Bishoyi, A.K., Sahoo, C.R. et al. Investigation of in vitro antimicrobial, antioxidant and antiproliferative activities of Nostoc calcicola biosynthesized gold nanoparticles. Bioprocess Biosyst Eng 46, 1341–1350 (2023). https://doi.org/10.1007/s00449-023-02905-1

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