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Investigation of the enhancement for Echinocandin B fermentation with methyl oleate from transcription level

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Abstract

Echinocandin B (ECB) is the key precursor compound of the antifungal drug Anidulafungin. The effects of the five precursor amino acids on ECB biosynthesis were firstly investigated. It showed that although L-threonine was a main compound of the hexapeptide scaffold of ECB, exogenous addition of L-threonine had no significant effect on the increase of ECB fermentation titer. Meanwhile, the ECB fermentation titer with methyl oleate showed two times higher than that of the other carbon sources. Transcription level analysis of the key genes for ECB biosynthesis indicated that the gene an655543 related to L-threonine biosynthesis showed higher value during the fermentation process, therefore, the exogenous addition of L-threonine had no obvious affection. Furthermore, it indicated that the transcription level of gene ecdA might be the main restriction factor for the ECB biosynthesis. The study provided the research foundation for the modification of the ECB producing strains in the following work.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

The authors gratefully acknowledge the financial supports of the National Key Research and Development Project of China (2022YFC2105400), Science and technology Project of Zhejiang Province (LGF22B060006).

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Authors and Affiliations

  1. The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, 310014, People’s Republic of China

    Kun Niu, Yu-Xin Qi, Hong-Wei Cai, Yi-Xin Ye, Hai-Yan Zhou, Xiao-Tian Liu, Zhi-Qiang Liu & Yu-Guo Zheng

  2. Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, People’s Republic of China

    Kun Niu, Yu-Xin Qi, Hong-Wei Cai, Yi-Xin Ye, Hai-Yan Zhou, Xiao-Tian Liu, Zhi-Qiang Liu & Yu-Guo Zheng

  3. School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai, 200235, People’s Republic of China

    Xiao-Tian Liu

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  1. Kun Niu
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Contributions

Z-QL and Y-GZ initiated and supervised the project; KN contributed to manuscript editing; Y-XQ conducted the experiments and data analysis; H-WC, Y-XY and X-TL participated in some of the experiments; H-YZ participated in the revision of the text. All the authors have read and approved the final manuscript.

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Correspondence to Zhi-Qiang Liu.

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Niu, K., Qi, YX., Cai, HW. et al. Investigation of the enhancement for Echinocandin B fermentation with methyl oleate from transcription level. Bioprocess Biosyst Eng 46, 1045–1052 (2023). https://doi.org/10.1007/s00449-023-02883-4

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  • DOI: https://doi.org/10.1007/s00449-023-02883-4

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