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Kinetic modeling of xylonic acid production by Gluconobacter oxydans: effects of hydrodynamic conditions

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Abstract

In this study, the synthesis of xylonic acid from xylose by Gluconobacter oxydans NL71 has been investigated. According to the relationship between oxygen transfer rate and oxygen uptake rate, three different kinetic models of product formation were established and the nonlinear fitting was carried out. The results showed that G. oxydans has critical dissolved oxygen under different strain concentrations, and the relationship between respiration intensity and dissolved oxygen conformed to the Monod equation \(q_{{{\text{O}}_{2} }} = q_{{{\text{O}}_{2} ,\max }} \frac{{C_{{\text{L}}} }}{{K_{{{\text{O}}_{2} }} + C_{{\text{L}}} }}\). The maximum reaction rate per unit cell mass and the theoretical maximum specific productivity of G. oxydans obtained by the kinetic model are 0.042 mol/L/h and 6.97 g/gx/h, respectively. These results will assist in determining the best balance between the airflow rate and cell concentration in the reaction and improve the production efficiency of xylonic acid.

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Data availability

The data that support the findings of this study are available from the corresponding author, Xu, upon reasonable request.

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Acknowledgements

This work was funded by the National Natural Science Foundation of China (No. 22208160), the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (No. 21KJB530015) and the National key research and development program (2021YFC2101602).

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Author notes

  1. Xu Liu and Chenrong Ding have contributed equally to this work.

Authors and Affiliations

  1. Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Jiangsu Provincial Key Lab for the Chemistry and Utilization of Agro-Forest Biomass, College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China

    Xu Liu, Chenrong Ding, Tao He, Yafei Zhu, Liqun Sun, Chaozhong Xu & Xiaoli Gu

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  1. Xu Liu
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  2. Chenrong Ding
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  3. Tao He
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  6. Chaozhong Xu
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  7. Xiaoli Gu
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Contributions

XL: investigation, data curation, writing—original draft. CD: investigation, data curation, writing—original draft. TH: validation, investigation, data curation. YZ: formal analysis. LS: formal analysis. CX: conceptualization, methodology, software, writing—review and editing, visualization, supervision, project administration, funding acquisition. XG: resources, supervision, project administration.

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Correspondence to Chaozhong Xu or Xiaoli Gu.

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Liu, X., Ding, C., He, T. et al. Kinetic modeling of xylonic acid production by Gluconobacter oxydans: effects of hydrodynamic conditions. Bioprocess Biosyst Eng 46, 829–837 (2023). https://doi.org/10.1007/s00449-023-02865-6

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