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Medium engineering of phenylethanoid transfructosylation catalysed by yeast β-fructofuranosidase

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Abstract

Tyrosol and hydroxytyrosol, by-products of olive oil production, are valuable substrates for enzymatic transglycosylation that can provide products with pharmaceutical potential. Phenylethanoid fructosides are produced from sucrose and phenylethanoids by the catalytic action of β-fructofuranosidases. This work dealt with the potential of the most abundant β-fructofuranosidase, baker's yeast invertase, for this bioconversion. The effects of sucrose and phenylethanoid concentrations were investigated with a focus on the selectivity of phenylethanoid transfructosylation and fructoside yields. For this purpose, initial rate and progress curve experiments were carried out for the initial (hydroxy)tyrosol and sucrose concentrations of 0.072–0.3 M and 1–2 M, respectively. Reaction courses exhibited either a maximum or plateau of fructoside yield in the range of about 10–18%. The addition of deep eutectic solvents was applied in the concentration range from 5 to 70% (v/v) to investigate the possibility of shifting the reaction equilibrium towards fructoside synthesis.

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The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This work was supported by grants from the Slovak Research and Development Agency (Grant No. APVV-18-0188) and the Slovak Grant Agency for Science (Grant No. VEGA 1/0515/22).

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Authors and Affiliations

  1. Department of Chemical and Biochemical Engineering, Faculty of Chemical and Food Technology, Institute of Chemical and Environmental Engineering, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia

    Klaudia Karkeszová, Monika Antošová & Milan Polakovič

  2. Institute of Chemistry, Slovak Academy of Sciences, Dúbravská Cesta 9, 845 38, Bratislava, Slovakia

    Elena Karnišová Potocká & Vladimír Mastihuba

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  1. Klaudia Karkeszová
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  2. Monika Antošová
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Correspondence to Milan Polakovič.

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Karkeszová, K., Antošová, M., Potocká, E.K. et al. Medium engineering of phenylethanoid transfructosylation catalysed by yeast β-fructofuranosidase. Bioprocess Biosyst Eng 46, 237–249 (2023). https://doi.org/10.1007/s00449-022-02828-3

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