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Co-immobilization of electron mediator and laccase onto dialdehyde starch cross-linked magnetic chitosan nanomaterials for organic pollutants’ removal

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Abstract

In this study, an amino-functionalized ionic liquid-modified magnetic chitosan (MACS-NIL) containing 2,2-diamine-di-3-ethylbenzothiazolin-6-sulfonic acid (ABTS) was used as a carrier, and dialdehyde starch (DAS) was used as a cross-linking agent to covalently immobilize laccase (MACS-NIL-DAS-lac), which realized the co-immobilization of laccase and ABTS. The carrier was characterized by Fourier infrared transform spectroscopy, scanning electron microscopy, thermogravimetric analysis, X-ray diffraction analysis, electron paramagnetic resonance, etc. The immobilization efficiency and activity retention of MACS-NIL-DAS-lac could reach 76.7% and 69.8%, respectively. At the same time, its pH stability, thermal stability, and storage stability had been significantly improved. In the organic pollutant removal performance test, the removal rate of 2,4-dichlorophenol (10 mg/L) by MACS-NIL-DAS-lac (1 U) could reach 100% within 6 h, and the removal efficiency could still reach 88.6% after six catalytic runs. In addition, MACS-NIL-DAS-lac also showed excellent degradation ability for other conventional phenolic pollutants and polycyclic aromatic hydrocarbons. The research results showed that MACS-NIL-DAS fabricated by the combination inorganic material, organic biomacromolecules, ionic liquid, and electron mediator could be used as a novel carrier for laccase immobilization and the immobilized laccase showed excellent removal efficiency for organic pollutants.

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Acknowledgements

This research was financially supported by National Natural Science Foundation of China (No. 22178174) and National key R&D program of China (No. 2021YFC2103802). The Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture (No. XTC2206), and Jiangsu Students’ platform for innovation and entrepreneurship training program (No. 202110291109Y).

Funding

This work was supported by the National Natural Science Foundation of China, under Grant 22178174, National key R&D Program of China, under Grant 2021YFC2103802, The Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture, under Grant XTC2206, and Jiangsu Students’ Platform for Innovation and Entrepreneurship Training Program, under Grant 202110291109Y.

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  1. State Key Laboratory of Materials-Oriented Chemical Engineering, School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, 210009, China

    Runtang Liu, Silin Wang, Mengyao Han, Wei Zhang, Huajin Xu & Yi Hu

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RL: conceptualization, methodology, formal analysis, investigation, data curation, writing–original draft, and writing–review & editing. SW methodology and investigation. MH methodology and investigation. WZ methodology and investigation. HX conceptualization, methodology, funding acquisition, and supervision. YH funding acquisition, supervision, and writing–review & editing.

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Liu, R., Wang, S., Han, M. et al. Co-immobilization of electron mediator and laccase onto dialdehyde starch cross-linked magnetic chitosan nanomaterials for organic pollutants’ removal. Bioprocess Biosyst Eng 45, 1955–1966 (2022). https://doi.org/10.1007/s00449-022-02799-5

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