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Bone morphogenetic protein (BMP)-modified graphene oxide-reinforced polycaprolactone–gelatin nanofiber scaffolds for application in bone tissue engineering

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Abstract

In this study, blend nanofibrous scaffolds were electrospun from polycaprolactone/gelatin (PCL/Gel) blend solutions reinforced by bone morphogenetic protein (BMP)-modified graphene oxide (GO). SEM results showed that uniform and bead-less nanofibers with 270 nm average diameter were obtained from electrospun of PCL/Gel blend solutions. Tensile strength test and contact angle measurement demonstrated that addition of PCL led to higher mechanical and physical properties of the resulting nanofibers. The addition of PCL as well as GO in the blend supports the suitable mechanical strength in the body media. The loading of BMP-modified graphene in the Gel/PCL structure caused the formation of nanofibrous substrate with great resemblance to bone tissue. Gel/PCL-G hybrid nanofibers revealed good biocompatibility in the presence of human osteosarcoma cells, and no trace of cellular toxicity was observed. The cells grown on the scaffolds exhibited a spindle-like and broad morphology and almost uniformly covered the entire nanofiber scaffold.

Graphical abstract

Gel/PCL nanofibers reinforced by graphene oxide-immobilized bone morphogenetic protein was prepared as a promising safe and biocompatible nanofiber with high antibacterial activity for bone tissue engineering.

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Authors and Affiliations

  1. Department of Dentistry, Kut University College, Kut, Wasit, 52001, Iraq

    Mustafa M. Kadhim

  2. College of Technical Engineering, The Islamic University, Najaf, Iraq

    Mustafa M. Kadhim

  3. Department of Pharmacy, Osol Aldeen University College, Baghdad, Iraq

    Mustafa M. Kadhim

  4. Institute of Pharmacy, Sechenov First Moscow State Medical University, 8 Trubetskaya St., bldg. 2, Moscow, 119991, Russian Federation

    Dmitry Olegovich Bokov

  5. Laboratory of Food Chemistry, Federal Research Center of Nutrition, Biotechnology and Food Safety, 2/14 Ustyinsky pr, Moscow, 109240, Russian Federation

    Dmitry Olegovich Bokov

  6. Department of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-kharj, Saudi Arabia

    Mohammad Javed Ansari

  7. Faculty of Nursing, HRH Princess Chulabhorn College of Medical Science, Chulabhorn Royal Academy, Bangkok, Thailand

    Wanich Suksatan

  8. Al-Nisour University College, Baghdad, Iraq

    Mohammed Abed Jawad

  9. Department of Occupational Therapy, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, 50200, Thailand

    Supat Chupradit

  10. College of Health and Medical Technology, Al-Ayen University, Thi-Qar, Iraq

    Mohammed N. Fenjan

  11. Department of Chemistry, College of Sciences, Shiraz University, 71946-84795, Shiraz, Iran

    Milad Kazemnejadi

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  1. Mustafa M. Kadhim
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Kadhim, M.M., Bokov, D.O., Ansari, M.J. et al. Bone morphogenetic protein (BMP)-modified graphene oxide-reinforced polycaprolactone–gelatin nanofiber scaffolds for application in bone tissue engineering. Bioprocess Biosyst Eng 45, 981–997 (2022). https://doi.org/10.1007/s00449-022-02717-9

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