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Transformation of Reactive Blue 19 by a recombinant peroxidase DyP

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Abstract

Wastewater containing recalcitrant dyes causes environmental problems. A new superfamily of heme-containing peroxidases, dye-decolorizing peroxidases (DyPs), has been found to decolorize different kinds of dyes, especial anthraquinone dyes efficiently. However, the mechanism of dyes degradation by DyPs has not been fully understood and the toxicity of dye degradation intermediates by DyPs catalysis to microbes is unclear. In this study, a purified recombinant Thermobifida fusca DyP (TfuDyP) in E. coli BL21(DE3) was used to treat Reactive Blue 19 (RB19), an anthraquinone dye. The reaction intermediates analyzed by ultra performance liquid chromatography/mass spectroscopy (UPLC–MS) indicated the initial site of TfuDyP attack on RB19. In addition, it was found that both RB19 and its incomplete degradation products inhibited the growth of Bacillus subtilis. These findings provided a novel understanding of DyPs catalysis to anthraquinone dyes.

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Acknowledgements

This work was supported by the Program of Introducing Talents of Discipline to Universities (1112-06), the National First-class Discipline Program of Light Industry Technology and Engineering (LITE2018-27), and Top-notch Academic Programs Project of Jiangsu Higher Education Institutions (PPZY2015A056).

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Authors and Affiliations

  1. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, No 1800 Lihu Avenue, Wuxi, 214122, Jiangsu, China

    Qian Pi, Zhubing Zhu & Lei Tang

  2. School of Biotechnology, Jiangnan University, No 1800 Lihu Avenue, Wuxi, 214122, Jiangsu, China

    Lei Tang

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  1. Qian Pi
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  2. Zhubing Zhu
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  3. Lei Tang
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Correspondence to Lei Tang.

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Pi, Q., Zhu, Z. & Tang, L. Transformation of Reactive Blue 19 by a recombinant peroxidase DyP. Bioprocess Biosyst Eng 45, 425–429 (2022). https://doi.org/10.1007/s00449-021-02660-1

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