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Development of bioactive solid support for immobilized Lactobacillus casei biofilms and the production of lactic acid

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Abstract

Polypropylene was modified to contain chitosan and evaluate its ability to generate Lactobacillus casei biofilms and their lactic acid production. Biofilm formation was carried out in either rich or minimal media. The chitosan-modified polypropylene harbored ~ 37% more cells than the control polypropylene. The biofilms from the chitosan-modified polypropylene grown in rich medium produced ~ 2 times more lactic acid after 72 h of incubation than the control suspended cells. There was no significant difference in the production of lactic acid after 72 h by L. casei biofilms on the chitosan-modified polypropylene grown in minimal media as compared with cells in suspension after 48 h and 72 h of incubation. Infrared spectroscopy confirmed higher deposition of nutrients and biomass on the chitosan-modified polypropylene as compared to the chitosan-free polypropylene. Electron and atomic force microscopy confirmed thicker biofilms when rich media were used to grow them as compared to minimal medium.

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Acknowledgements

Authors would like to thank Dr. John Yun from SI group, Inc. for providing Polybond 7200 and Dr. FenAnn Shen from the Microscopy Core Facility at Utah State University (Logan, UT) for assistance in the Scanning Electron Microscopy analyses. This work was supported by the Utah Agricultural Experiment Station UTA01377 and approved as Journal Paper 9500.

Funding

Utah Agricultural Experiment Station project UTA01377.

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Authors and Affiliations

  1. Department of Nutrition, Dietetics and Food Sciences, Utah State University, Logan, UT, USA

    Luis J. Bastarrachea & Robert E. Ward

  2. Department of Biological Engineering, Utah State University, Logan, UT, USA

    David W. Britt

  3. Department of Agricultural and Biological Engineering, Pennsylvania State University, University Park, PA, USA

    Ali Demirci

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  1. Luis J. Bastarrachea
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  2. David W. Britt
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  4. Ali Demirci
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Contributions

LJB designed and executed experiments, authors DWB and REW aided in surface and metabolite analytical techniques, author AD provided additional assistance in experimental design and results interpretation.

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Correspondence to Luis J. Bastarrachea.

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Bastarrachea, L.J., Britt, D.W., Ward, R.E. et al. Development of bioactive solid support for immobilized Lactobacillus casei biofilms and the production of lactic acid. Bioprocess Biosyst Eng 45, 217–226 (2022). https://doi.org/10.1007/s00449-021-02654-z

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