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Xylanolytic enzyme consortia from Bacillus sp. NIORKP76 for improved biobleaching of kraft pulp

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Abstract

A cellulase-free xylanolytic enzyme consortia consisting of a xylanase, arabinofuranosidase, and acetyl xylan esterase produced by Bacillus sp. NIORKP76 isolate under solid-state fermentation was assessed for its bio-bleaching ability on kraft pulp. In the biobleaching analysis, the xylanase dose of 5 Ug−1 dry pulp denoted the optimum bleaching of pulp at 40 °C and pH 8.0 after 2 h of treatment. The reduction in kappa number of pre-treated hardwood pulp using xylanolytic enzyme consortium (XEC) was found to be ~ 55%, while solo xylanase could reduce the kappa number to 44–46%. In the case of chemical bagasse pulp, a reduction of ~ 27.5% and 19–20% was seen in kappa number using XEC and solo xylanase, respectively. Enzyme-treated pulp (HW and CB) showed a 50% reduction in hypochlorite consumption during the chlorine treatment. The current study results reveal the significant potential of xylanolytic enzyme consortium from Bacillus sp. NIORKP76 on the environmentally friendly bio-bleaching process.

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Acknowledgements

The authors are thankful to Director, CSIR-National Institute of Oceanography, Goa (India), and Head, BOD, for giving all necessary services and encouragement. The authors thank the Council of Scientific and Industrial Research (CSIR, India) for providing financial support through the Senior Research Fellowship (SRF) to the first author. This article is part of doctoral work being submitted by the first author at Goa University in the Department of Microbiology under the corresponding author's guidance. This publication has CSIR-NIO contribution number 6790.

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  1. Biological Oceanography Division, CSIR-National Institute of Oceanography, Dona Paula, Goa, 403004, India

    Pankaj Parab & Rakhee Khandeparker

  2. Department of Microbiology, Goa University, Taleigao Plateau, Goa, 403206, India

    Pankaj Parab

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449_2021_2623_MOESM1_ESM.tif

Supplementary file1 Fig. 1 Correlation between brightness development and lignin content of kraft pulp samples (TIF 166 KB)

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Parab, P., Khandeparker, R. Xylanolytic enzyme consortia from Bacillus sp. NIORKP76 for improved biobleaching of kraft pulp. Bioprocess Biosyst Eng 44, 2513–2524 (2021). https://doi.org/10.1007/s00449-021-02623-6

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