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Bacterial strain for bast fiber crops degumming and its bio-degumming technique

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Abstract

The research and development of bio-degumming technology is under a slow progress due to the shortage of proper efficient bacterial strains and processes. A degumming bacterial strain—Pectobacterium wasabiae (PW)—with broad-spectrum degumming abilities was screened out in this study. After the fermentation for 12 h, the residual gum contents of kenaf bast, ramie bast, hemp bast, flax bast, and Apocynum venetum bast were all lower than 15%. This bacterial strain could realize the simultaneous extracellular secretion of pectinase, mannase, and xylanase with the maximum enzyme activity levels of 130.25, 157.58, and 115.24 U/mL, respectively. The optimal degumming conditions of this bacterial strain were as follows: degumming time of 12 h, bath ratio of 1:10, temperature of 33 °C, and inoculum size of 2%. After the bio-degumming through this bacterial strain, the COD in wastewater was below 4000 mg/L, which was over 60% lower than that in boiling-off wastewater generated by chemical degumming. This technology achieves higher efficiency, higher quality, and lower pollution.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (No. 31871675), China Agriculture Research System (CARS-19-E22), the Natural Science Foundation of Hunan Province (No. 2019JJ40332), Chinese Agricultural Science and Technology Innovation Project (ASTIP-IBFC08), and Central Public-interest Scientific Institution Basal Research Fund (No.1610242021002).

Funding

This study was supported by the National Natural Science Foundation of China (No. 31871675), China Agriculture Research System (CARS-19-E22), the Natural Science Foundation of Hunan Province (No. 2019JJ40332), Chinese Agricultural Science and Technology Innovation Project (ASTIP-IBFC08), and Central Public-interest Scientific Institution Basal Research Fund (No.1610242021002).

Author information

Authors and Affiliations

  1. Institute of Bast Fiber Crops, Chinese Academy of Agriculture Sciences, Changsha, China

    Shengwen Duan, Lifeng Cheng, Xiangyuan Feng, Qi Yang, Ke Zheng, Mingqiang Gao, Zhiyuan Liu, Chunjie Liu & Yuande Peng

  2. Huzhou Nanxun Shanlian Shengye Textile Co., Ltd, Huzhou, 313014, China

    Bingrong Xu

Authors
  1. Shengwen Duan
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  2. Bingrong Xu
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  3. Lifeng Cheng
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  4. Xiangyuan Feng
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  5. Qi Yang
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  6. Ke Zheng
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  7. Mingqiang Gao
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  8. Zhiyuan Liu
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  9. Chunjie Liu
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  10. Yuande Peng
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Contributions

SD: Methodology, Investigation, Writing—original draft. BX: Methodology, Investigation, Writing—original draft. Lifeng Cheng: Supervision, Conceptualization, Writing—review and editing. XF: Supervision, Conceptualization, Writing and review. QY: Formal analysis, Software, Validation. KZ: Formal analysis, Software, Validation. MG: Formal analysis, Software, Validation. ZL: Formal analysis, Software, Validation. CL: Supervision, Conceptualization, Writing—review and editing. YP: Supervision, Conceptualization, Writing—review and editing.

Corresponding authors

Correspondence to Chunjie Liu or Yuande Peng.

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The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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The strain Pectobacterium wasabiae PW was preserved in China General Microbiological Culture Collection Center (CGMCC), with the preservation number of CGMCC No. 14601.

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Duan, S., Xu, B., Cheng, L. et al. Bacterial strain for bast fiber crops degumming and its bio-degumming technique. Bioprocess Biosyst Eng 44, 2503–2512 (2021). https://doi.org/10.1007/s00449-021-02622-7

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  • DOI: https://doi.org/10.1007/s00449-021-02622-7

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