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Investigation of enzymes and solvents in the production process of 6-ammonium penicillanic acid (6-APA) in industry to reduce costs and improve production conditions

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Abstract

In this study, the optimization of the amount of enzyme consumed in the enzymatic phase of substitution of butanol solvent instead of methanol in the powder washing phase after filtration was investigated. To perform this study, different amounts of the enzyme penicillin G amidase (PGA) were tested in reactions with the same conditions. The highest efficiency was observed in the reaction that the ratio of penicillin powder to the amount of enzyme was 2:1. In this reaction, for every 100 g of penicillin consumed, 50 g of the PGA was used. Replacement of butanol instead of methanol after filtration, the powder obtained from this step was washed with butanol instead of methanol and the powder obtained from this step was examined after drying. The resulting solvent powder was very small and the drying speed of the powder increased compared to the time of methanol usage. Optimizing the amount of enzyme in this process due to the high cost of the enzyme made this reaction more economically viable at the end of this study. In this study, for the first time, butanol was used as a suitable substitute for methanol and the ratio of enzyme use to penicillin powder was optimized. This research deals with the future perspective in the field of research in this regard.

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Authors and Affiliations

  1. Department of Organic Chemistry, Faculty of Pharmaceutical Chemistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

    Sajjad Fereidouni Velasejerdi

  2. Department of Chemical Engineering, North Tehran Branch, Islamic Azad University, P.O. Box 1651153311, Tehran, Iran

    Akbar Esmaeili

  3. Department of Chemistry, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Abdolhosein Rustaiyan

Authors
  1. Sajjad Fereidouni Velasejerdi
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  2. Akbar Esmaeili
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  3. Abdolhosein Rustaiyan
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by SFV. The first draft of the manuscript was written by AE, and AR.

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Correspondence to Akbar Esmaeili.

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Fereidouni Velasejerdi, S., Esmaeili, A. & Rustaiyan, A. Investigation of enzymes and solvents in the production process of 6-ammonium penicillanic acid (6-APA) in industry to reduce costs and improve production conditions. Bioprocess Biosyst Eng 44, 2481–2489 (2021). https://doi.org/10.1007/s00449-021-02619-2

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  • DOI: https://doi.org/10.1007/s00449-021-02619-2

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