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Thermodynamics, kinetics and optimization of catalytic behavior of polyacrylamide-entrapped carboxymethyl cellulase (CMCase) for prospective industrial use

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Abstract

In the current study, kinetic and thermodynamic parameters of free and polyacrylamide-immobilized CMCase were analyzed. The maximum immobilization yield of 34 ± 1.7% was achieved at 11% acrylamide. The enthalpy of activation (ΔH) of free and immobilized enzyme was found to be 13.61 and 0.29 kJ mol−1, respectively. Irreversible inactivation energy of free and immobilized CMCase was 96.43 and 99.01 kJ mol−1, respectively. Similarly, the enthalpy of deactivation (ΔHd) values for free and immobilized enzyme were found to be in the range of 93.51–93.76 kJ mol−1 and 96.08–96.33 kJ mol−1, respectively. Michaelis–Menten constant (Km) increased from 1.267 ± 0.06 to 1.5891 ± 0.07 mg ml−1 and the maximum reaction rate (Vmax) value decreased (8319.47 ± 416 to 5643.34 ± 282 U ml−1 min−1) after immobilization. Due to wide pH and temperature stability profile with sufficient reusing efficiency up to three successive cycles, the immobilized CMCase might be useful for various industrial processes.

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Funding

The authors thank KIBGE, University of Karachi, for providing facilities for the experimental work.

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Authors and Affiliations

  1. Dr. Panjwani Center for Molecular Medicine and Drug Research, Jamil-Ur-Rahman Center for Genome Research, International Center for Chemical and Biological Sciences University of Karachi, Karachi, 75270, Pakistan

    Asad Karim

  2. Department of Biotechnology, Federal Urdu University of Arts, Science and Technology (FUUAST), Gulshan-e-Iqbal Campus, Karachi, 75300, Pakistan

    Zainab Bibi

  3. Department of Biotechnology, Shaheed Benazir Bhutto University, Dir (Upper), Sheringal, Pakistan

    Muhammad Asif Nawaz

  4. The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan

    Afsheen Aman

  5. Department of Biochemistry, University of Karachi, Karachi, Pakistan

    Shah Ali Ul Qader

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Karim, A., Bibi, Z., Nawaz, M.A. et al. Thermodynamics, kinetics and optimization of catalytic behavior of polyacrylamide-entrapped carboxymethyl cellulase (CMCase) for prospective industrial use. Bioprocess Biosyst Eng 44, 2417–2427 (2021). https://doi.org/10.1007/s00449-021-02614-7

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