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Improved thermal stability of phytase from Yersinia intermedia by physical adsorption immobilization on amino-multiwalled carbon nanotubes

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Abstract

Phytase is used in poultry diets to hydrolyze and release of phytate-bound phosphorus. Immobilization on nanomaterials optimizes enzyme’s thermal stability and reusability. This study aimed to immobilize the recombinant phytase from Yersinia intermedia on the surface of amino-multi-walled carbon nanotubes (amino-MWCNTs) by physical adsorption. For this, zeta potential measurement, FTIR spectroscopic analysis, scanning electron microscope (SEM), kinetic as well as thermodynamic parameters were used to characterize immobilized phytase on amino-MWCNTs. According to results, the optimum temperature of the immobilized phytase increased from 50 to 70 °C and also thermal and pH stability improved considerably. Moreover, immobilization led to an increase in the value of Km and kcat from 0.13 to 0.33 mM and 2220 to 2776 s−1, respectively. In addition, the changes in activation energy of thermal inactivation (ΔE#a (D)), the free energy of thermal inactivation (ΔG#D) and the enthalpy of thermal inactivation (ΔH#D) for immobilized phytase increased by +11.05, +24.7 and +11.4 kj/mole, respectively, while the value of the change in the entropy of thermal inactivation (ΔS#D) decreased by − 0.04 kj/mole.K. Overall, our results showed that adsorption immobilization of phytase on amino-MWCNTs increases thermal, pH and storage stability as well as some of kinetic parameters.

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Acknowledgements

The authors wish to thank Shahrkord University for the use of facilities and financial support.

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Authors and Affiliations

  1. Department of Biology, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran

    Sima Lahiji, Roohullah Hemmati & Mansoureh Ghorbani

  2. Biotechnology Research Institute, Shahrekord University, Shahrekord, Iran

    Roohullah Hemmati

  3. Department of Marine Biology, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran

    Ahmad Homaei

  4. Department of Genetics, Faculty of Basic Sciences, Shahrekord University, Sharekord, Iran

    Behnaz Saffar

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  1. Sima Lahiji
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  2. Roohullah Hemmati
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  3. Ahmad Homaei
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  5. Mansoureh Ghorbani
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Correspondence to Roohullah Hemmati.

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Lahiji, S., Hemmati, R., Homaei, A. et al. Improved thermal stability of phytase from Yersinia intermedia by physical adsorption immobilization on amino-multiwalled carbon nanotubes. Bioprocess Biosyst Eng 44, 2217–2228 (2021). https://doi.org/10.1007/s00449-021-02598-4

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  • DOI: https://doi.org/10.1007/s00449-021-02598-4

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