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Succinic anhydride-based chemical modification making laccase@Cu3(PO4)2 hybrid nanoflowers robust in removing bisphenol A in wastewater

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Abstract

To prepare a robust biocatalyst and enhance the removal of bisphenol A in wastewater, succinic anhydride was reacted with laccase to obtain succinic anhydride-modified laccase (SA-laccase) and then co-crystallized with Cu3(PO4)2 to form SA-laccase@Cu3(PO4)2 hybrid nanoflowers (hNFs). The activity of SA-laccase@Cu3(PO4)2 reached 5.27 U/mg, 1.86-, 2.88- and 2.15-fold those of bare laccase@Cu3(PO4)2, laccase@Ca3(PO4)2 and laccase@epoxy resin, respectively. Compared with free laccase, the obtained hNFs present enhanced activity and tolerance to pH and high temperature in the removal of BPA. Under the optimum conditions of pH 6.0 and 35 °C, BPA removal reached 93.2% using SA-laccase@Cu3(PO4)2 hNFs, which was 1.21-fold of that using free laccase. In addition, the obtained SA-laccase@Cu3(PO4)2 hNFs retained nearly 90% of their initial catalytic activity for BPA removal after 8 consecutive batch cycles. This efficient method for preparing immobilized laccase can also be further developed and improved to acquire green biocatalysts for removing persistent organic pollutants in wastewater.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (22078079, 81730108), the Natural Science Foundation of Zhejiang Province (LY18B060009), the National Innovation and Entrepreneurship Training Program for Undergraduate (201810346008), the “Star and light” Project for Talent Students in Hangzhou Normal University (2019) and Research Plan for Sprout Talents in University in Zhejiang Province (2020R427071).

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  1. Huafang Yang, Peipei He and Youcheng Yin contributed equally to this work.

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  1. College of Materials, Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, 311121, People’s Republic of China

    Huafang Yang, Peipei He, Zhili Mao, Jing Zhang, Changle Zhong & Anming Wang

  2. College of Medicine, Hangzhou Normal University, Hangzhou, 311121, People’s Republic of China

    Youcheng Yin & Tian Xie

  3. College of Materials, Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, 310036, People’s Republic of China

    Tian Xie & Anming Wang

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Yang, H., He, P., Yin, Y. et al. Succinic anhydride-based chemical modification making laccase@Cu3(PO4)2 hybrid nanoflowers robust in removing bisphenol A in wastewater. Bioprocess Biosyst Eng 44, 2061–2073 (2021). https://doi.org/10.1007/s00449-021-02583-x

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