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Hacking biofilm developed in a structured-bed reactor (SBRRIA) with integrated processes of nitrogen and organic matter removal

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Abstract

Biomass samples from a structured-bed reactor subjected to recirculation and intermittent aeration (SBRRIA) were analyzed to investigate the bacterial community shift along with the changes in the C/N ratio. The C/N ratios tested were 7.6 ± 1.0 (LNC) and 2.9 ± 0.4 (HNC). The massive sequencing analyses revealed that the microbial community adjusted itself to different organic and nitrogenous applied loads, with no harm to reactor performance regarding COD and Total-N removal. Under LNC, conventional nitrification and heterotrophic denitrification steered the process, as indicated by the detection of microorganisms affiliated with Nitrosomonadaceae, Nitrospiraceae, and Rhodocyclaceae families. However, under HNC, the C/N ratio strongly affected the microbial community, resulting in the prevalence of members of Saprospiraceae, Chitinophagaceae, Xanthomonadaceae, Comamonadaceae, Bacillaceae, and Planctomycetaceae. These families include bacteria capable of using organic matter derived from cell lysis, ammonia-oxidizers under low DO, heterotrophic nitrifiers–aerobic denitrifiers, and non-isolated strains of Anammox. The DO profile confirmed that the stratification in aerobic, anoxic, and anaerobic zones enabled the establishment of different nitrogen degradation pathways, including the Anammox.

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Acknowledgements

The authors gratefully acknowledge financial support from FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo, Brazil) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil) (Processes 2013/15665-8 and 2015/21650-9). The authors especially thank the Technician Antônio Wagner Lamon from Laboratory of Microsensors in the São Carlos School of Engineering (EESC/USP, Brazil) for constructing the DO microsensor and performing this analysis.

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  1. Environmental Engineering Department, Federal University of Triângulo Mineiro (UFTM), Av. Dr. Randolfo Borges Júnior 1250, Univerdecidade, Uberaba, Minas Gerais, 38064-200, Brazil

    Carla Eloísa Diniz dos Santos

  2. Department of Biochemistry and Organic Chemistry, Institute of Chemistry, São Paulo State University (UNESP), R. Francisco Degni, 55, Araraquara, São Paulo, 14800-060, Brazil

    Rachel Biancalana Costa

  3. Biological Processes Laboratory, São Carlos School of Engineering (EESC), University of São Paulo (USP), Av. João Dagnone 1100, Santa Angelina, São Carlos, São Paulo, 13563-120, Brazil

    Carla Eloísa Diniz dos Santos, Camila Abreu Borges Silva Rabelo, Eloísa Pozzi, Eugenio Foresti & Márcia Helena Rissato Zamariolli Damianovic

  4. Brazilian Biorenewables National Laboratory (LNBR/CNPEM), Rua Giuseppe Máximo Scolfaro, 10.000, Polo II de Alta Tecnologia, Campinas, São Paulo, 13083-970, Brazil

    Antônio Djalma Nunes Ferraz Júnior & Gabriela Felix Persinoti

  5. Laboratorio de Ecología Microbiana, Departamento de Bioquímica Y Genómica Microbiana, Instituto de Investigaciones Biológicas Clemente Estable, Av. Italia 3318, Montevideo, Uruguay

    Antônio Djalma Nunes Ferraz Júnior

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dos Santos, C.E.D., Costa, R.B., Rabelo, C.A.B.S. et al. Hacking biofilm developed in a structured-bed reactor (SBRRIA) with integrated processes of nitrogen and organic matter removal. Bioprocess Biosyst Eng 44, 1841–1851 (2021). https://doi.org/10.1007/s00449-021-02564-0

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