Dual effects of hypoxia on proliferation and osteogenic differentiation of mouse clonal mesenchymal stem cells


Mouse clonal mesenchymal stem cells (mc-MSCs) were cultured on a Cytodex 3 microcarrier in a spinner flask for a suspension culture under hypoxia condition to increase mass productivity. The hypoxia environment was established using 4.0 mM Na2SO3 with 10 μM or 100 µM CoCl2 for 24 h in a low glucose DMEM medium. As a result, the proliferation of mc-MSCs under hypoxic conditions was 1.56 times faster than the control group over 7 days. The gene expression of HIF-1a and VEGFA increased 4.62 fold and 2.07 fold, respectively. Furthermore, the gene expression of ALP, RUNX2, COL1A, and osteocalcin increased significantly by 9.55, 1.55, 2.29, and 2.53 times, respectively. In contrast, the expression of adipogenic differentiation markers, such as PPAR-γ and FABP4, decreased. These results show that the hypoxia environment produced by these chemicals in a suspension culture increases the proliferation of mc-MSCs and promotes the osteogenic differentiation of mc-MSCs.

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This study was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (NRF-2020R1A4A1016793), the National Research Foundation of Korea (NRF-2020R1F1A1048494), and Inha University Research Grant, Korea.

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Correspondence to Soonjo Kwon.

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Kim, H., Kwon, S. Dual effects of hypoxia on proliferation and osteogenic differentiation of mouse clonal mesenchymal stem cells. Bioprocess Biosyst Eng (2021). https://doi.org/10.1007/s00449-021-02563-1

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  • Hypoxia
  • Mesenchymal stem cells
  • Proliferation
  • Differentiation
  • Microcarrier culture