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Facile synthesis of catalase@ZIF-8 composite by biomimetic mineralization for efficient biocatalysis

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Abstract

Enzymes immobilized in metal–organic frameworks (MOFs) have attracted great attention as a promising hybrid material. In the study, a novel biomimetic mineralization encapsulation process for a highly stable and easily reusable catalase (CAT)@ZIF-8 composite has been designed. This immobilization process provides a high enzyme loading of 70 wt %. The CAT@ZIF-8 composites exhibited a much lower Km value and better enzyme activity than those of free CAT, exhibiting good stability against enzymatic hydrolysis and protein denaturation under harsh conditions. The inhibitory effects of pesticides such as pH, temperature, solvent (i.e., methanol, dimethyl sulfoxide and tetrahydrofuran) and storage at room temperature (6 months) on the activity of free and immobilized catalase enzyme were investigated. The CAT@MOF composites also exhibited excellent reusability, an obvious advantage for treating a wastewater from food processing. The CAT@MOF developed is promising for the efficient removal of H2O2 under harsh conditions.

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Acknowledgements

The authors wish to acknowledge the financial support from National Natural Science Foundation of China (No: 31270620), Fundamental Research Funds for the Central Universities (No. DUT19JC13) and Dalian Scientific and Technological Innovation Foundation (No: 2018J12SN072).

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Authors and Affiliations

  1. Key Laboratory of Industrial Ecology and Environmental Engineering, School of Ocean Science and Technology, Dalian University of Technology, Ministry of Education, Panjin, 124221, China

    Feng Guo, Zhonghao Xu, Wendong Zhang, Tongxin Wang, Xiaoxuan Di, Qian Zhang & Zihan Zhu

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  1. Feng Guo
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  2. Zhonghao Xu
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  3. Wendong Zhang
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  4. Tongxin Wang
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Correspondence to Feng Guo.

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Guo, F., Xu, Z., Zhang, W. et al. Facile synthesis of catalase@ZIF-8 composite by biomimetic mineralization for efficient biocatalysis. Bioprocess Biosyst Eng 44, 1309–1319 (2021). https://doi.org/10.1007/s00449-021-02540-8

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