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Biochemical characterization and enhanced production of endoxylanase from thermophilic mould Myceliophthora thermophila

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Abstract

Endoxylanase production from M. thermophila BJTLRMDU3 using rice straw was enhanced to 2.53-fold after optimization in solid state fermentation (SSF). Endoxylanase was purified to homogeneity employing ammonium sulfate precipitation followed by gel filtration chromatography and had a molecular mass of ~ 25 kDa estimated by SDS-PAGE. Optimal endoxylanase activity was recorded at pH 5.0 and 60 °C. Purified enzyme showed complete tolerance to n-hexane, but activity was slightly inhibited by other organic solvents. Among surfactants, Tweens (20, 60, and 80) and Triton X 100 slightly enhanced the enzyme activity. The Vmax and Km values for purified endoxylanase were 6.29 µmol/min/mg protein and 5.4 mg/ml, respectively. Endoxylanase released 79.08 and 42.95% higher reducing sugars and soluble proteins, respectively, which control after 48 h at 60 °C from poultry feed. Synergistic effect of endoxylanase (100 U/g) and phytase (15 U/g) on poultry feed released higher amount of reducing sugars (58.58 mg/feed), soluble proteins (42.48 mg/g feed), and inorganic phosphate (28.34 mg/feed) in contrast to control having 23.55, 16.98, and 10.46 mg/feed of reducing sugars, soluble proteins, and inorganic phosphate, respectively, at 60 °C supplemented with endoxylanase only.

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Acknowledgements

SD acknowledges the Maharshi Dayanand University, Rohtak for providing financial support in the form of University Research Scholarship (URS) during the course of investigation. The Department of Science and Technology [No. 1196 SR/FST/LS-I/2017/4] is highly acknowledged for providing basic infrastructure facility at M.D.U., Rohtak for carrying out this research work.

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Authors and Affiliations

  1. Laboratory of Bioprocess Technology, Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana, 124001, India

    Seema Dahiya & Bijender Singh

  2. Department of Botany, Pt. N. R. S. Govt. College, Rohtak, Haryana, 124001, India

    Anil Kumar

  3. Department of Zoology, Maharshi Dayanand University, Rohtak, Haryana, 124001, India

    Vinay Malik

  4. Department of Chemistry, Central University of Haryana, Jant-Pali, Mahendergarh, Haryana, 123031, India

    Vinod Kumar

  5. Department of Biotechnology, Central University of Haryana, Jant-Pali, Mahendergarh, Haryana, 123031, India

    Bijender Singh

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Dahiya, S., Kumar, A., Malik, V. et al. Biochemical characterization and enhanced production of endoxylanase from thermophilic mould Myceliophthora thermophila. Bioprocess Biosyst Eng 44, 1539–1555 (2021). https://doi.org/10.1007/s00449-021-02539-1

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