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Mixotrophic growth regime as a strategy to develop microalgal bioprocess from nutrimental composition of tequila vinasses

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Abstract

The selection of a suitable growth regime can increase the physiological performance of microalgae and improve bioprocess based on these microorganisms from agro-industrial residues. Thus, this study assessed the biotechnology capacity—biomass production, biochemical composition, and nutrient uptake—from tequila vinasses (TVs) as the nutrient source of three indigenous microalgae—Chlorella sp., Scenedesmus sp., and Chlamydomonas sp.—cultured under heterotrophic and mixotrophic conditions. The results demonstrated that under the mixotrophic regime, the three microalgae evaluated reached the highest nitrogen uptake, biomass production, and cell compound accumulation. Under this condition, Chlorella sp. and Scenedesmus sp. showed the highest nutrient uptake and biomass production, 1.7 ± 0.3 and 1.9 ± 0.3 g L−1, respectively; however, the biochemical composition, mainly carbohydrates and proteins, varied depending on the microalgal strain and its growth regime. Overall, our results demonstrated the biotechnological capacity of native microalgae from TVs, which may vary not only depending on the microalgal strain but also the culture strategy implemented and the characteristics of the residue used, highlighting—from a perspective of circular bio-economy—the feasibility of implementing microalgal bioprocess to reuse and valorize the nutrimental composition of TVs through biomass and high-valuable metabolite production, depicting a sustainable strategy for tequila agro-industry in Mexico.

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Data availability

The datasets generated during and/or analyses during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

Francisco J. Choix is grateful to Consejo Nacional de Ciencia y Tecnologia (CONACYT) for the support under the Program-Cátedras CONACYT; external analysis service of CUCEI-UdG for the analysis of TVs, and Diana Fischer for editorial services.

Funding

This study was funded by PROSNI 243338-2018 Program-Universidad de Guadalajara.

Author information

Authors and Affiliations

  1. Departamento de Ingeniería Química, CUCEI-Universidad de Guadalajara, Blvd. M. García Barragán 1421, CP 44430, Guadalajara, Jalisco, México

    Francisco J. Choix & José Roberto Ramos-Ibarra

  2. Centro de Investigación, Asistencia en Tecnología y Diseño del Estado de Jalisco A.C, Camino Arenero 1227, CP 45019, Zapopan, Jalisco, México

    Pedro Mondragón-Cortez

  3. Departamento de Ecología, CUCBA-Universidad de Guadalajara, Ramón Padilla Sánchez 2100, CP 45200, Zapopan, Jalisco, México

    Martha Alicia Lara-González, Eduardo Juárez-Carrillo, Amayaly Becerril-Espinosa & Héctor Ocampo-Alvarez

  4. Instituto de Limnología, CUCBA-Universidad de Guadalajara, Paseo de la loma 22, CP 45920, Ajijic, Jalisco, México

    Eduardo Juárez-Carrillo

  5. CONACYT, CUCEI-Universidad de Guadalajara, Blvd. M. García Barragán 1421, CP 44430, Guadalajara, Jalisco, México

    Francisco J. Choix

  6. CONACYT, CUCBA-Universidad de Guadalajara, Ramón Padilla Sánchez 2100, CP 45200, Zapopan, Jalisco, México

    Amayaly Becerril-Espinosa

  7. Tecnológico Nacional de México/I. T. del Valle del Yaqui, Academy of Biology, Av. Tecnológico, Block 611, Bácum, Sonora, México

    Jony R. Torres

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  1. Francisco J. Choix
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  2. José Roberto Ramos-Ibarra
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  4. Martha Alicia Lara-González
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Correspondence to Francisco J. Choix.

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Choix, F.J., Ramos-Ibarra, J.R., Mondragón-Cortez, P. et al. Mixotrophic growth regime as a strategy to develop microalgal bioprocess from nutrimental composition of tequila vinasses. Bioprocess Biosyst Eng 44, 1155–1166 (2021). https://doi.org/10.1007/s00449-021-02512-y

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