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Bacterial cellulose as an oleaginous yeast cell carrier for soybean oil refinery effluent treatment and pyrolysis oil production

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Abstract

Bacterial cellulose produced from soybean oil refinery effluent is a good immobilization carrier because of the large pores in its fiber network, its high water-holding capacity, and its good biocompatibility. In this study, it was applied to immobilization of oleaginous yeasts for treating soybean oil refinery effluent. The immobilization percentage reached 50%, and the removal of chemical oxygen demand and oil content reached 92.1% and 93.1%, respectively, during dynamic immobilization using a mass percentage of bacterial cellulose of 30% and an immobilization time of 24 h, which were significantly higher than those of free oleaginous yeasts or yeasts immobilized by bacterial cellulose from rich medium. The immobilized oleaginous yeasts facilitated the recovery of the yeasts and effectively treated three batches of soybean oil refinery effluent. The immobilized oleaginous yeasts recovered after soybean oil refinery effluent treatment were pyrolyzed to produce bio-oil, which contributed to more alkanes and a higher calorific value of bio-oil in the pyrolysis products as compared to those of free oleaginous yeasts. As bacterial cellulose used as an oleaginous yeast cell carrier is produced from soybean oil refinery effluent, no waste of immobilization materials is involved and an efficient waste-into-oil bioprocess is developed.

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References

  1. Dourou M, Kancelista A, Juszczyk P, Sarris D, Bellou S, Triantaphyllidou IE, Rywinska A, Papanikolaou S, Aggelis G (2016) Bioconversion of olive mill wastewater into high-added value products. J Clean Prod 139:957–969. https://doi.org/10.1016/j.jclepro.2016.08.133

    Article  CAS  Google Scholar 

  2. Frazão CJR, Silva NHC, Freire CSR, Silvestre AJD, Xavier AMRB, Tavares APM (2014) Bacterial cellulose as carrier for immobilization of laccase: optimization and characterization. Eng Life Sci 14(5):500–508

    Article  Google Scholar 

  3. Huang C, Guo HJ, Xiong L, Wang B, Shi SL, Chen XF, Lin XQ, Wang C, Luo J, Chen XD (2016) Using wastewater after lipid fermentation as substrate for bacterial cellulose production by Gluconacetobacter xylinus. Carbohydr Polym 136:198–202. https://doi.org/10.1016/j.carbpol.2015.09.043

    Article  CAS  PubMed  Google Scholar 

  4. Imran A, Bramer EA, Seshan K, Brem G (2016) Catalytic flash pyrolysis of biomass using different types of zeolite and online vapor fractionation. Energies 9(3):187. https://doi.org/10.3390/en9030187

    Article  CAS  Google Scholar 

  5. Jung HI, Lee OM, Jeong JH, Jeon YD, Park KH, Kim HS, An WG, Son HJ (2010) Influence of glycerol on production and structural–physical properties of cellulose from Acetobacter sp. V6 cultured in shake flasks. Bioresour Technol 101(10):3602–3608

    Article  CAS  Google Scholar 

  6. Kim HJ, Jin JN, Kan E, Kim KJ, Lee SH (2017) Bacterial cellulose−chitosan composite hydrogel beads for enzyme immobilization. Biotechnol Bioproc E 22(1):89–94

    Article  CAS  Google Scholar 

  7. Kirdponpattara S, Phisalaphong M (2013) Bacterial cellulose–alginate composite sponge as a yeast cell carrier for ethanol production. Biochem Eng J 77:103–109. https://doi.org/10.1016/j.bej.2013.05.005

    Article  CAS  Google Scholar 

  8. Liang HW, Guan QF, Zhu Z, Song LT, Yao HB, Lei X, Yu SH (2012) Highly conductive and stretchable conductors fabricated from bacterial cellulose. NPG Asia Mater 4(6):e19–e19

    Article  Google Scholar 

  9. Ling J, Tian Y, Toledo RAD, Shim H (2017) Cost reduction for the lipid production from distillery and domestic mixed wastewater by Rhodosporidium toruloides via the reutilization of spent seed culture medium. Energy 136:135–141. https://doi.org/10.1016/j.energy.2016.04.008

    Article  CAS  Google Scholar 

  10. Muradov N, Taha M, Miranda AF, Wrede D, Kadali K, Gujar A, Stevenson T, Ball AS, Mouradov A (2015) Fungal-assisted algal flocculation: application in wastewater treatment and biofuel production. Biotechnol Biofuels 8:24. https://doi.org/10.1186/s13068-015-0210-6

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Nguyen DN, Ton NMN, Le VVM (2009) Optimization of Saccharomyces cerevisiae immobilization in bacterial cellulose by ‘adsorption−incubation’ method. Int Food Res J 16:59–64

    CAS  Google Scholar 

  12. Qiao N, Fan X, Zhang XZ, Shi YF, Wang L, Yu DY (2019) Soybean oil refinery effluent treatment and its utilization for bacterial cellulose production by Gluconacetobacter xylinus. Food Hydrocoll 97:105185. https://doi.org/10.1016/j.foodhyd.2019.105185

    Article  CAS  Google Scholar 

  13. Qiao N, Gao MX, Zhang XZ, Du YD, Fan X, Wang L, Liu N, Yu DY (2019) Trichosporon fermentans biomass flocculation from soybean oil refinery wastewater using bioflocculant produced from Paecilomyces sp. M2–1. Appl Microbiol Biotechnol 103:2821–2831. https://doi.org/10.1007/s00253-019-09643-z

    Article  CAS  PubMed  Google Scholar 

  14. Rajkumar K, Muthukumar M, Sivakumar R (2010) Novel approach for the treatment and recycle of wastewater from soya edible oil refinery industry—an economic perspective. Resour Conserv Recycl 54(10):752–758

    Article  Google Scholar 

  15. Sarris D, Stoforos NG, Mallouchos A, Kookos IK, Koutinas AA, Aggelis G, Papanikolaou S (2017) Production of added-value metabolites by Yarrowia lipolytica growing in olive mill wastewater-based media under aseptic and non-aseptic conditions. Eng Life Sci 17(6):695–709

    Article  CAS  Google Scholar 

  16. Sharma DMS, Sharma DA, Verma DS, Dodiya HS (2014) Treatment of edible oil refinery waste water by using chemical and biological process. Int J Eng Sci Res Technol 3(9):115–120

    Google Scholar 

  17. Shen Q, Lin H, Wang Q, Fan XP, Yang YY, Zhao YH (2015) Sweetpotato vines hydrolysate promotes single cell oils production of Trichosporon fermentans in high-density molasses fermentation. Bioresour Technol 176:249–256. https://doi.org/10.1016/j.biortech.2014.11.045

    Article  CAS  PubMed  Google Scholar 

  18. Shi YF, Ren HM, Yu DY (2018) Research progress on treatment of wastewater by airlift loop Reactor. J Northeast Electr Power Univ 38(4):76–84

    Google Scholar 

  19. Shi YF, Wu S, Ren HM, Jin MT, Wang L, Qiao N, Yu DY (2020) Computational fluid dynamics and factor analysis of a novel swirling demulsified airlift loop reactor for the treatment of refined soybean oil wastewater. Bioresour Technol 296:122316. https://doi.org/10.1016/j.biortech.2019.122316

    Article  CAS  PubMed  Google Scholar 

  20. Ton NMN, Le VVM (2011) Application of immobilized yeast in bacterial cellulose to the repeated batch fermentation in wine-making. Int Food Res J 18(3):983–987

    CAS  Google Scholar 

  21. Wang LP, Schütz C, Salazar-Alvarez G, Titirici MM (2014) Carbon aerogels from bacterial nanocellulose as anodes for lithium ion batteries. RSC Adv 4(34):17549. https://doi.org/10.1039/c3ra47853j

    Article  CAS  Google Scholar 

  22. Yang XY, Wang X, Zhao BW, Li Y (2014) Simulation model of pyrolysis biofuel yield based on algal components and pyrolysis kinetics. Bioenergy Res 7(4):1293–1304

    Article  CAS  Google Scholar 

  23. Yao WY, Wu X, Zhu J, Sun B, Zhang YY, Miller C (2011) Bacterial cellulose membrane—a new support carrier for yeast immobilization for ethanol fermentation. Process Biochem 46(10):2054–2058

    Article  CAS  Google Scholar 

  24. Yin N, Santos TMA, Auer GK, Crooks JA, Oliver PM, Weibel DB (2014) Bacterial cellulose as a substrate for microbial cell culture. Appl Environ Microb 80(6):1926–1932

    Article  Google Scholar 

  25. Yu DY, Hu S, Liu WS, Wang XN, Jiang HF, Dong NH (2020) Pyrolysis of oleaginous yeast biomass from wastewater treatment:kinetics analysis and biocrude characterization. Renew Energy 150:831–839. https://doi.org/10.1016/j.renene.2020.01.028

    Article  CAS  Google Scholar 

  26. Yu DY, Hu S, Wang L, Chen QC, Dong NH (2020) Comparative study on pyrolysis characteristics andkinetics of oleaginous yeast and algae. Int J Hydrogen Energy 45:10979–10990. https://doi.org/10.1016/j.ijhydene.2020.02.052

    Article  CAS  Google Scholar 

  27. Yu DY, Wang XN, Fan X, Ren HM, Hu S, Wang L, Shi YF, Liu N, Qiao N (2018) Refined soybean oil wastewater treatment and its utilization for lipid production by the oleaginous yeast Trichosporon fermentans. Biotechnol Biofuels 11:299. https://doi.org/10.1186/s13068-018-1306-6

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Yu DY, Yang MY, Gao B, Liu WC (2011) Disposal of domestic sewage by heterotrophic nitrification−aerobic denitrifier bacteria immobilized with bacterial cellulose membrane. Fresen Environ Bull 20(2):398–404

    CAS  Google Scholar 

  29. Yu N, Xing DF, Li W, Yang Y, Li Z, Li YR, Ren NQ (2017) Electricity and methane production from soybean edible oil refinery wastewater using microbial electrochemical systems. Int J Hydrogen Energy 42(1):96–102

    Article  CAS  Google Scholar 

  30. Zhao HW, Xia J, Wang JM, Yan XF, Wang C, Lei TZ, Xian M, Zhang HB (2018) Production of bacterial cellulose using polysaccharide fermentation wastewater as inexpensive nutrient sources. Biotechnol Biotechnol Equip 32(2):350–356

    Article  CAS  Google Scholar 

  31. Żywicka A, Peitler D, Rakoczy R, Junka AF, Fijałkowski K (2016) Wet and dry forms of bacterial cellulose synthetized by different strains of Gluconacetobacter xylinus as carriers for yeast immobilization. Appl Biochem Biotechnol 180(4):805–816

    Article  Google Scholar 

  32. Żywicka A, Wenelska K, Junka A, Czajkowska J, Fijałkowski K (2019) An efficient method of Yarrowia lipolytica immobilization using oil- and emulsion-modified bacterial cellulose carriers. Electron J Biotechnol 41:30–36. https://doi.org/10.1016/j.ejbt.2019.06.002

    Article  CAS  Google Scholar 

  33. Żywicka A, Wenelska K, Junka A, Chodaczek G, Szymczyk P, Fijałkowski K (2019) Immobilization pattern of morphologically different microorganisms on bacterial cellulose membranes. World J Microbiol Biotechnol 35:11. https://doi.org/10.1007/s11274-018-2584-7

    Article  CAS  PubMed  Google Scholar 

Download references

Funding

This work was supported by grants from the National Natural Science Foundation of China (31470787, 21708003) and Department of Science and Technology of Jilin Province (20190902014TC, 20170519015JH).

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Authors and Affiliations

  1. School of Civil Engineering and Architecture, Northeast Electric Power University, Jilin, 132012, China

    Nan Qiao, Xue Fan & Yundi Du

  2. School of Resources and Environmental Engineering, Jilin Institute of Chemical Technology, Jilin, 132022, China

    Xue Fan

  3. Sci-Tech Center for Clean Conversion and High-Valued Utilization of Biomass, Jilin Province, Northeast Electric Power University, Jilin, 132012, Jilin, China

    Shuang Hu, Xiuzhen Zhang, Ling Wang, Lei Wang, Xiaojun Zhang & Dayu Yu

Authors
  1. Nan Qiao
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  2. Xue Fan
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  3. Shuang Hu
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  4. Xiuzhen Zhang
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  5. Ling Wang
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  7. Lei Wang
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  8. Xiaojun Zhang
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  9. Dayu Yu
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Correspondence to Xiaojun Zhang or Dayu Yu.

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Qiao, N., Fan, X., Hu, S. et al. Bacterial cellulose as an oleaginous yeast cell carrier for soybean oil refinery effluent treatment and pyrolysis oil production. Bioprocess Biosyst Eng 44, 661–671 (2021). https://doi.org/10.1007/s00449-020-02476-5

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  • DOI: https://doi.org/10.1007/s00449-020-02476-5

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