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Biotransformation of two citrus flavanones by lactic acid bacteria in chemical defined medium

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Abstract

Microbial processes are being developed to transform flavonoid glycosides to varieties of metabolites with higher bioavailability. The aim of this study was to determine the metabolic activity and survival of five lactic acid bacteria (LAB) stains (L. rhamnosus LRa05, L. casei LC89, L. plantarum N13, L. acidophilus LA85, and L. brevis LB01) in two different citrus flavanone standards (hesperetin-7-O-rutinoside and naringenin-7-O-rutinoside). The enzymatic activity, metabolites, antioxidant activities, and α-glucosidase inhibition property in the two standards were also investigated before and after incubated with LAB. The medium contained standards permitted survival of the five LAB stains. All strains exhibited β-glucosidase activity. Of the five LAB strains tested, just L. plantarum N13 and L. brevis LB01 have the ability to metabolize hesperetin-7-O-rutinoside, only L. plantarum N13, L. acidophilus LA85, and L. brevis LB01 could metabolize naringenin-7-O-rutinoside, moreover, L. acidophilus LA85l was the strain with the highest biotransformation ratio of naringenin-7-O-rutinoside. L. acidophilus LA85 and L. plantarum N13 can degrade naringenin-7-O-rutinoside into naringenin. L. brevis LB01 can degrade hesperetin-7-O-rutinoside into hesperetin, 3-(4′-hydroxyphenyl)-2-propenoic acid, 3-(3′-hydroxy-4′-methoxyphenyl)hydracrylic acid, and 3-(4′-hydroxyphenyl)propionic acid. Incubation of L. acidophilus LA85 in naringenin-7-O-rutinoside solution supposed no apparent influence in the biological activities that tested. L. acidophilus LA85 may potentially contribute to the bioavailability of citrus flavanones, and to be applied as functional cultures to obtain more bioavailable and bioactive metabolites in food products or in the human gastrointestinal tract.

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Acknowledgements

Thanks Jiangsu Wecare Biotechnology Co., Ltd (Suzhou, China) for providing lactic acid bacteria strains. This research was supported by Fundamental Research Funds for the National Natural Science Foundation of China (31771947). The requirement of managing all communication between the journal and all co-authors during submission and proofing are delegated to Submitting Author.

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Authors and Affiliations

  1. College of Food Science and Technology, Huazhong Agricultural University, 1 Shizishan Road, Wuhan, 430070, People’s Republic of China

    Xiao Guo, Ailing Guo & Erhu Li

  2. Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, Wuhan, 430070, Hubei, People’s Republic of China

    Ailing Guo & Erhu Li

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  1. Xiao Guo
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  2. Ailing Guo
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  3. Erhu Li
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Correspondence to Erhu Li.

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449_2020_2437_MOESM1_ESM.tif

Supplementary Figure 1 Extracted ion chromatograms of bacterial flavanone metabolites. (A) 72 h incubation of naringenin-7-O-rutinoside (20 mg/L) with L. acidophilus LA85, (B) 72 h incubation of naringenin-7-O-rutinoside (20 mg/L) with L. plantarum N13, (C) 72 h incubation of hesperetin-7-O-rutinoside with L. brevis LB01 (TIF 946 kb)

449_2020_2437_MOESM2_ESM.tif

Supplementary Figure 2 Chromatograms (A270nm) of 72 h incubation of naringenin-7-O-rutinoside (20 mg/L) with L. acidophilus LA85 (black line), 72 h incubation of naringenin-7-O-rutinoside (20 mg/L) without L. acidophilus LA85 (blue line, control 1), 72 h incubation of L. acidophilus LA85 without naringenin-7-O-rutinoside (green line, control 2). Peak 1: naringenin-7-O-rutinoside, peak 2: naringenin (TIF 736 kb)

449_2020_2437_MOESM3_ESM.tif

Supplementary Figure 3 Chromatograms (A270nm) of 72 h incubation of naringenin-7-O-rutinoside (20 mg/L) with L. plantarum N13 (black line), 72 h incubation of naringenin-7-O-rutinoside (20 mg/L) without L. plantarum N13 (blue line, control 1), 72 h incubation of L. plantarum N13 without naringenin-7-O-rutinoside (green line, control 2). Peak 1: naringenin-7-O-rutinoside, peak 2: naringenin (TIF 738 kb)

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Guo, X., Guo, A. & Li, E. Biotransformation of two citrus flavanones by lactic acid bacteria in chemical defined medium. Bioprocess Biosyst Eng 44, 235–246 (2021). https://doi.org/10.1007/s00449-020-02437-y

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