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Green biomimetic silver nanoparticles utilizing the red algae Amphiroa rigida and its potent antibacterial, cytotoxicity and larvicidal efficiency

Bioprocess and Biosystems Engineering volume 44, pages 217–223 (2021)Cite this article

Abstract

The present investigation reports a simple, rapid, inexpensive, and eco-friendly approach for synthesizing Amphiroa rigida-mediated silver nanoparticles (AR-AgNPs) for the first time. The biomimetic synthesized AR-AgNPs were characterized by both spectral and microscopic analysis. The UV–visible spectrum showed the surface plasmon peak at 420 nm, which indicated the formation of AR-AgNPs. X-ray diffraction characterization of AR-AgNPs showed a face-centered cubic crystal (25 nm) and the transmission electron microscope micrograph showed spherical shape. The functional group of polysaccharide that plays a major role as a stabilizing and reducing agent is confirmed by Fourier-transform infrared spectroscopy. The biomimetic synthesized AR-AgNPs showed antibacterial activity against Staphylococcus aureus (21 ± 0.2 mm) and Pseudomonas aeruginosa (15 ± 0.2 mm). Further, the cytotoxic effects of AR-AgNPs against MCF-7 human breast cancer cells were observed through acridine orange-ethidium bromide and Hoechst staining. Besides, AR-AgNPs are found to be inhibit the growth of 3rd and 4th instar larvae of Aedes aegypti in a dose-dependent manner.

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Authors and Affiliations

  1. PG and Research Department of Biotechnology, Mahendra Arts and Science College (Autonomous), Kalippatti, Namakkal, Tamil Nadu, 637 501, India

    Mani Gopu, Thangasamy Selvankumar, Chinnappan Sudhakar & Kandasamy Selvam

  2. Food Chemistry and Molecular Cancer Biology Laboratory, Department of Animal Health and Management, Alagappa University, Karaikudi, Tamil Nadu, 630 003, India

    Ponnuchamy Kumar

  3. Department of Medical Microbiology, College of Health and Medical Sciences, Haramaya University, P.O. Box 235, Harar, Ethiopia

    Balakrishnan Senthilkumar

  4. Department of Environmental Engineering, Kyungpook National University, Daegu, 41566, Republic of Korea

    Muthusamy Govarthanan

  5. PG and Research Department of Chemistry, Mahendra Arts and Science College (Autonomous), Kalippatti, Namakkal, Tamil Nadu, 637 501, India

    Rathakrishnan Selva Kumar

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  1. Mani Gopu
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  2. Ponnuchamy Kumar
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  3. Thangasamy Selvankumar
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  4. Balakrishnan Senthilkumar
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Correspondence to Kandasamy Selvam.

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Gopu, M., Kumar, P., Selvankumar, T. et al. Green biomimetic silver nanoparticles utilizing the red algae Amphiroa rigida and its potent antibacterial, cytotoxicity and larvicidal efficiency. Bioprocess Biosyst Eng 44, 217–223 (2021). https://doi.org/10.1007/s00449-020-02426-1

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