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Lignolytic and hemicellulolytic enzyme cocktail production from Bacillus tequilensis LXM 55 and its application in pulp biobleaching

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Abstract

Bioprocessing of pulp requires lignolytic as well as hemicellulolytic enzymes. The present study is the first report of a cocktail of laccase (L), xylanase (X), and mannanase (M), from a single bacterium for pulp biobleaching. A novel strain Bacillus tequilensis LXM 55 produced thermo-alkali stable L + X + M. On optimization higher enzyme yield (IUml−1/fold increase) of laccase (396.35/24.16), xylanase (212.95/81.90) and mannanase (153.33/102.90) were achieved in the cocktail. Treatment of pulp with cocktail of enzymes led to 49.35% reduction in kappa number and considerable enhancement in the brightness (11.59%), whiteness (4.11%), and other pulp properties. Most importantly, no mediator system was required for the application of laccase. 40% less chlorine consumption was required to obtain the paper of the same quality as that of pulp treated without enzyme but with 100% chlorine. Therefore, this cocktail of enzymes is highly suitable for pulp biobleaching in the paper mill.

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Acknowledgements

The financial support provided by University Grant Commission (UGC-MRP-43-476/2014/SR) and (UGC-SAP II-DRS-I) New Delhi, India is acknowledged.

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Authors and Affiliations

  1. Department of Microbiology, Panjab University, BMS Block-I, South Campus, Sector-25, Chandigarh, 160014, India

    Steffy Angural, Aditya Kumar, Deepak Kumar, Rahul Warmoota & Naveen Gupta

  2. Department of Biotechnology, Chandigarh College of Technology, Landran, Mohali, 140307, India

    Sonica Sondhi

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  1. Steffy Angural
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  2. Aditya Kumar
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  3. Deepak Kumar
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Correspondence to Naveen Gupta.

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Angural, S., Kumar, A., Kumar, D. et al. Lignolytic and hemicellulolytic enzyme cocktail production from Bacillus tequilensis LXM 55 and its application in pulp biobleaching. Bioprocess Biosyst Eng 43, 2219–2229 (2020). https://doi.org/10.1007/s00449-020-02407-4

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