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Enhanced volatile fatty acid production from sago hampas by Clostridium beijerinckii SR1 for bioelectricity generation using microbial fuel cells

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Abstract

Sago hampas is a starch-based biomass from sago processing industries consisted of 58% remaining starch. This study has demonstrated the bioconversion of sago hampas to volatile fatty acids (VFAs) by Clostridium beijerinckii SR1 via anaerobic digestion. Higher total VFAs were obtained from sago hampas (5.04 g/L and 0.287 g/g) as compared to commercial starch (5.94 g/L and 0.318 g/g). The physical factors have been investigated for the enhancement of VFAs production using one-factor-at-a-time (OFAT). The optimum condition; 3% substrate concentration, 3 g/L of yeast extract concentration and 2 g/L of ammonium nitrate enhanced the production of VFAs by 52.6%, resulted the total VFAs produced is 7.69 g/L with the VFAs yield of 0.451 g/g. VFAs hydrolysate produced successfully generated 273.4 mV of open voltage circuit and 61.5 mW/m2 of power density in microbial fuel cells. It was suggested that sago hampas provide as an alternative carbon feedstock for bioelectricity generation.

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Abbreviations

C/N:

Carbon per nitrogen

PEM:

Proton exchange membrane

OFAT:

One factor at a time

RCM:

Reinforced clostridium media

VFAs:

Volatile fatty acids

YE:

Yeast extract

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Acknowledgements

The authors gratefully acknowledge the MyBrain 15 from Malaysian Higher Education Ministry for the financial support. We would like to share our greatest attitude to all the members of the Environmental Biotechnology Research Group, Universiti Putra Malaysia for their help and support.

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  1. Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Mohd Azwan Jenol, Mohamad Faizal Ibrahim, Ezyana Kamal Bahrin & Suraini Abd-Aziz

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Jenol, M.A., Ibrahim, M.F., Kamal Bahrin, E. et al. Enhanced volatile fatty acid production from sago hampas by Clostridium beijerinckii SR1 for bioelectricity generation using microbial fuel cells. Bioprocess Biosyst Eng 43, 2027–2038 (2020). https://doi.org/10.1007/s00449-020-02391-9

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