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Separation of microbial oil produced by Mortierella isabellina using polymeric membranes

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Abstract

The objective of this work was to concentrate, through a membrane separation process, the fatty acids from oil/solvent mixture. The oil was obtained by ultrasound-assisted extraction from freeze-dried cells of Mortierella isabellina. The concentration of the fatty acids was investigated using flat-sheet polymer membranes of ultrafiltration and nanofiltration. The effects of temperature and pressure were evaluated by the retention of the fatty acids. Oil retentions between 45.23 and 58.20% to ultrafiltration membrane and 43.50 and 56.00% to nanofiltration membrane were observed. The best condition for the ultrafiltration membrane was 4 bar and 40 °C and for nanofiltration membrane was 12 bar and 50 °C. The oil contains a high concentration of oleic acid and palmitic acid that is a desirable property for the biodiesel production. The results showed the applicability of this technology in the solvent recovery step whereas the oil recovered contains a high concentration of fatty acids.

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Acknowledgements

The authors thank the National Council for Scientific and Technological Development (CNPq) and the Coordination for the Improvement of Higher Education Personnel (CAPES) for the financial support.

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Authors and Affiliations

  1. Department of Chemical Engineering, Federal University of Santa Maria, Av. Roraima, 1000, Santa Maria, RS, 97105-900, Brazil

    Daniela Sallet, Lauren Theisen Fischer, Marcio Antonio Mazutti & Raquel Cristine Kuhn

  2. Department of Crop Protection, Federal University of Santa Maria, Roraima Av., 1000, Santa Maria, RS, 97105-900, Brazil

    Gustavo Ugalde

  3. Laboratory of Agroindustrial Processes Engineering (LAPE), Federal University of Santa Maria, Sete de Setembro St., 1040, Cachoeira do Sul, 96508-010, Brazil

    Marcus Vinícius Tres

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  1. Daniela Sallet
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  2. Lauren Theisen Fischer
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  3. Gustavo Ugalde
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  4. Marcus Vinícius Tres
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  5. Marcio Antonio Mazutti
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  6. Raquel Cristine Kuhn
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Correspondence to Daniela Sallet.

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Sallet, D., Fischer, L.T., Ugalde, G. et al. Separation of microbial oil produced by Mortierella isabellina using polymeric membranes. Bioprocess Biosyst Eng 43, 1943–1949 (2020). https://doi.org/10.1007/s00449-020-02383-9

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  • DOI: https://doi.org/10.1007/s00449-020-02383-9

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