Skip to main content

Advertisement

Springer Nature Link
Log in

Optimization of immobilization conditions for Lactobacillus pentosus cells

  • Research Paper
  • Published:
Bioprocess and Biosystems Engineering Aims and scope Submit manuscript

Abstract

In this study, the immobilization technology was used to improve the LA yield and shorten the fermentation time. The optimum conditions to immobilize Lactobacillus pentosus ATCC 8041 cell were determined by Taguchi design L16 (45). The immobilized L. pentosus ATCC 8041 cells prepared by 2% sodium alginate (SA) and 6% polyvinyl alcohol (PVA) with the immobilization process by 0.10 M calcium chloride (CaCl2) and 2.5% boric acid (H3BO3) had the best performance of LA yield at the temperature of 35 °C, which is significantly higher than that of L. pentosus ATCC 8041 free cells. These cells maintained the stable and efficient performance in 15 repeated batch fermentation, and they also have excellent mechanical strength to keep from breakage caused by cell growth and agitation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

References

  1. Chibata I, Wingard LB (2014) Immobilized microbial cells: applied biochemistry and bioengineering, vol 4. Elsevier, New York

    Google Scholar 

  2. Tao XQ, Lu GN, Liu JP, Li T, Yang LN (2009) Rapid degradation of phenanthrene by using Sphingomonas sp. GY2B immobilized in calcium alginate gel beads. Int J Environ Res Public Health 6(9):2470–2480

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Kong HJ, Smith MK, Mooney DJ (2003) Designing alginate hydrogels to maintain viability of immobilized cells. Biomaterials 24(22):4023–4029

    Article  CAS  PubMed  Google Scholar 

  4. Grasdalen H, Larsen B, Smisrod O (1981) 13C-NMR studies of monomeric composition and sequence in alginate. Carbohyd Res 89(2):179–191

    Article  CAS  Google Scholar 

  5. Lee KY, Mooney DJ (2012) Alginate: properties and biomedical applications. Prog Polym Sci 37(1):106–126

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Tang Y, Pang L, Wang D (2017) Preparation and characterization of borate bioactive glass cross-linked PVA hydrogel. J Non-Cryst Solids 476:25–29

    Article  CAS  Google Scholar 

  7. Zhan J, Jiang S, Pan L (2013) Immobilization of phospholipase A1 using a polyvinyl alcohol-alginate matrix and evaluation of the effects of immobilization. Braz J Chem Eng 30(4):721–728

    Article  CAS  Google Scholar 

  8. Xue L, Huang Z, Luo Z, Cao C, Chen Y, Chen R (2009) Optimization of the techniques of S. cerevisiae immobilization by sodium alginate and PVA. Liquor Mak Sci Technol 2:27–30

    Google Scholar 

  9. Wang H, Seki M, Furusaki S (1995) Mathematical model for analysis of mass transfer for immobilized cells in lactic acid fermentation. Biotechnol Prog 11(5):558–564

    Article  CAS  PubMed  Google Scholar 

  10. Vijayakumar J, Aravindan R, Viruthagiri T (2008) Recent trends in the production, purification and application of lactic acid. Chem Biochem Eng Q 22(2):245–264

    CAS  Google Scholar 

  11. Bustos G, Moldes AB, Cruz JM, Domínguez JM (2005) Influence of the metabolism pathway on lactic acid production from hemicellulosic trimming vine shoots hydrolyzates using Lactobacillus pentosus. Biotechnol Prog 21(3):793–798

    Article  CAS  PubMed  Google Scholar 

  12. Garde A, Jonsson G, Schmidt AS, Ahring BK (2002) Lactic acid production from wheat straw hemicellulose hydrolysate by Lactobacillus pentosus and Lactobacillus brevis. Biores Technol 81(3):217–223

    Article  CAS  Google Scholar 

  13. Kourkoutas Y, Xolias V, Kallis M, Bezirtzoglou E, Kanellaki M (2005) Lactobacillus casei cell immobilization on fruit pieces for probiotic additive, fermented milk and lactic acid production. Process Biochem 40(1):411–416

    Article  CAS  Google Scholar 

  14. Abdel-Rahman MA, Tashiro Y, Sonomoto K (2013) Recent advances in lactic acid production by microbial fermentation processes. Biotechnol Adv 31(6):877–902

    Article  CAS  PubMed  Google Scholar 

  15. Taguchi G, Yokoyama Y (1993) Taguchi methods: design of experiments, vol 4. Amer Supplier Institute, Cairo

    Google Scholar 

  16. Khalid I, Ahmad M, Minhas MU, Barkat K (2018) Preparation and characterization of alginate-PVA-based semi-IPN: controlled release pH-responsive composites. Polym Bull 75(3):1075–1099

    Article  CAS  Google Scholar 

  17. Zhu GL, Hu YY, Wang QR (2009) Nitrogen removal performance of anaerobic ammonia oxidation co-culture immobilized in different gel carriers. Water Sci Technol 59(12):2379–2386

    Article  CAS  PubMed  Google Scholar 

  18. García EA, Montoro BP, Benomar N, Castillo-Gutiérrez S, Estudillo-Martínez MD, Knapp CW, Abriouel H (2019) New insights into the molecular effects and probiotic properties of Lactobacillus pentosus pre-adapted to edible oils. LWT 109:153–162

    Article  CAS  Google Scholar 

  19. Lee KH, Choi IS, Kim YG, Yang DJ, Bae HJ (2011) Enhanced production of bioethanol and ultrastructural characteristics of reused Saccharomyces cerevisiae immobilized calcium alginate beads. Biores Technol 102(17):8191–8198

    Article  CAS  Google Scholar 

  20. Bubb WA (2003) NMR spectroscopy in the study of carbohydrates: characterizing the structural complexity. Concepts Magn Reson Part A Educ J 19(1):1–19

    Google Scholar 

  21. Holzgrabe U (2010) Quantitative NMR spectroscopy in pharmaceutical applications. Prog Nucl Magn Reson Spectrosc 57(2):229–240

    Article  CAS  PubMed  Google Scholar 

  22. Sanders ER (2012) Aseptic laboratory techniques: plating methods. J Vis Exp 63:e3064

    Google Scholar 

  23. Gjersing E, Happs RM, Sykes RW, Doeppke C, Davis MF (2013) Rapid determination of sugar content in biomass hydrolysates using nuclear magnetic resonance spectroscopy. Biotechnol Bioeng 110(3):721–728

    Article  CAS  PubMed  Google Scholar 

  24. Mittal A, Scott GM, Amidon TE, Kiemle DJ, Stipanovic AJ (2009) Quantitative analysis of sugars in wood hydrolyzates with 1H NMR during the autohydrolysis of hardwoods. Biores Technol 100(24):6398–6406

    Article  CAS  Google Scholar 

  25. Buyondo JP (2013) Kinetic studies of lactic acid production from wood extract hydrolysate via batch and continuous fermentation processes. State University of New York College of Environmental Science and Forestry

  26. Nord LI, Vaag P, Duus JØ (2004) Quantification of organic and amino acids in beer by 1H NMR spectroscopy. Anal Chem 76(16):4790–4798

    Article  CAS  PubMed  Google Scholar 

  27. Bouteille R, Gaudet M, Lecanu B, This H (2013) Monitoring lactic acid production during milk fermentation by in situ quantitative proton nuclear magnetic resonance spectroscopy. J Dairy Sci 96(4):2071–2080

    Article  CAS  PubMed  Google Scholar 

  28. Arabi M, Ghaedi M, Ostovan A, Tashkhourian J, Asadallahzadeh H (2016) Synthesis and application of molecularly imprinted nanobeads combined ultrasonic assisted for highly selective solid phase extraction trace amount of celecoxib from human plasma samples using design expert (DXB) software. Ultrason Sonochem 33:67–76

    Article  CAS  PubMed  Google Scholar 

  29. Goksungur Y, Guvenc U (1999) Production of lactic acid from beet molasses by calcium alginate immobilized Lactobacillus delbrueckii IFO 3202. J Chem Technol Biotechnol 74(2):131–136

    Article  CAS  Google Scholar 

  30. Gilson CD, Thomas A (1995) Ethanol production by alginate immobilised yeast in a fluidised bed bioreactor. J Chem Technol Biotechnol Int Res Process Environ Clean Technol 62(1):38–45

    CAS  Google Scholar 

  31. Najafpour G, Younesi H, Ismail KSK (2004) Ethanol fermentation in an immobilized cell reactor using Saccharomyces cerevisiae. Biores Technol 92(3):251–260

    Article  CAS  Google Scholar 

  32. Chrastil J (1991) Gelation of calcium alginate. Influence of rice starch or rice flour on the gelation kinetics and on the final gel structure. J Agric Food Chemistry 39(5):874–876

    Article  CAS  Google Scholar 

  33. Blandino A, Macias M, Cantero D (1999) Formation of calcium alginate gel capsules: influence of sodium alginate and CaCl2 concentration on gelation kinetics. J Biosci Bioeng 88(6):686–689

    Article  CAS  PubMed  Google Scholar 

  34. Idris A, Suzana W (2006) Effect of sodium alginate concentration, bead diameter, initial pH and temperature on lactic acid production from pineapple waste using immobilized Lactobacillus delbrueckii. Process Biochem 41(5):1117–1123

    Article  CAS  Google Scholar 

  35. Bandi S, Kunamneni A, Polur E (2003) Investigation on neomycin production with immobilized cell of Streptomyces marinensis Vuv-5 in calcium alginate matrix. AAP Pharm Sci Tech 20:220

    Google Scholar 

  36. Seifan M, Samani AK, Hewitt S, Berenjian A (2017) The effect of cell immobilization by calcium alginate on bacterially induced calcium carbonate precipitation. Fermentation 3(4):57

    Article  CAS  Google Scholar 

  37. Tian X, Liao Q, Liu W, Wang YZ, Zhu X, Li J, Wang H (2009) Photo-hydrogen production rate of a PVA-boric acid gel granule containing immobilized photosynthetic bacteria cells. Int J Hydrogen Energy 34(11):4708–4717

    Article  CAS  Google Scholar 

  38. Bhatnagar Y, Singh GB, Mathur A, Srivastava S, Gupta S, Gupta N (2016) Biodegradation of carbazole by Pseudomonas sp. GBS. 5 immobilized in polyvinyl alcohol beads. J Biochem Technol 6(3):1003–1007

    Google Scholar 

  39. Wang P, Chen Z, Li J, Wang L, Gong G, Zhao G, Liu H, Zheng Z (2013) Immobilization of Rhizopus oryzae in a modified polyvinyl alcohol gel for L (+)-lactic acid production. Ann Microbiol 63(3):957–964

    Article  CAS  Google Scholar 

  40. Jamali NS, Rashidi NFD, Jahim JM, Sompong O, Jehlee A, Engliman NS (2019) Thermophilic biohydrogen production from palm oil mill effluent: effect of immobilized cells on granular activated carbon in fluidized bed reactor. Food Bioprod Process 117:231–240

    Article  CAS  Google Scholar 

  41. Sakoda JM, Cohen BH, Beall G (1954) Test of significance for a series of statistical tests. Psychol Bull 51(2):172

    Article  CAS  PubMed  Google Scholar 

  42. Delgado A, Brito D, Peres C, Noe-Arroyo F, Garrido-Fernández A (2005) Bacteriocin production by Lactobacillus pentosus B96 can be expressed as a function of temperature and NaCl concentration. Food Microbiol 22(6):521–528

    Article  CAS  Google Scholar 

  43. Liu J, Pan D, Wu X, Chen H, Cao H, Li QX, Hua R (2018) Enhanced degradation of prometryn and other s-triazine herbicides in pure cultures and wastewater by polyvinyl alcohol-sodium alginate immobilized Leucobacter sp. JW-1. Sci Total Environ 615:78–86

    Article  CAS  PubMed  Google Scholar 

  44. Kurade MB, Waghmode TR, Xiong J-Q, Govindwar SP, Jeon B-H (2019) Decolorization of textile industry effluent using immobilized consortium cells in upflow fixed bed reactor. J Clean Prod 213:884–891

    Article  CAS  Google Scholar 

  45. Watanabe I, Miyata N, Ando A, Shiroma R, Tokuyasu K, Nakamura T (2012) Ethanol production by repeated-batch simultaneous saccharification and fermentation (SSF) of alkali-treated rice straw using immobilized Saccharomyces cerevisiae cells. Biores Technol 123:695–698

    Article  CAS  Google Scholar 

  46. John RP, Nampoothiri KM, Pandey A (2007) Production of L (+) lactic acid from cassava starch hydrolyzate by immobilized Lactobacillus delbrueckii. J Basic Microbiol 47(1):25–30

    Article  CAS  PubMed  Google Scholar 

  47. Wang Z, Wang Y, Yang ST, Wang R, Ren H (2010) A novel honeycomb matrix for cell immobilization to enhance lactic acid production by Rhizopus oryzae. Biores Technol 101(14):5557–5564

    Article  CAS  Google Scholar 

  48. Senthuran A, Senthuran V, Mattiasson B, Kaul R (1997) Lactic acid fermentation in a recycle batch reactor using immobilized Lactobacillus casei. Biotechnol Bioeng 55(6):841–853

    Article  CAS  PubMed  Google Scholar 

  49. Brodelius P (1985) The potential role of immobilization in plant cell biotechnology. Trends Biotechnol 3(11):280–285

    Article  CAS  Google Scholar 

  50. Vatakit T, Leenanon B (2017) Effects of prebiotics and cells encapsulation on survivability of Lactobacillus pentosus PR01 in probiotic fermented purple glutinous rice beverage. Asian J Microbiol Biotechnol Environ Exp Sci 19:1–10

    Google Scholar 

  51. Maier RM, Pepper IL (2015) Bacterial growth. Environmental microbiology. Elsevier, Oxford, pp 37–56

    Chapter  Google Scholar 

  52. Shinmyo A, Kimura H, Okada H (1982) Physiology of α-amylase production by immobilized Bacillus amyloliquefaciens. Eur J Appl Microbiol Biotechnol 14(1):7–12

    Article  CAS  Google Scholar 

  53. Chen CC, Lan CC, Pan CL, Huang MY, Chew CH, Hung CC, Chen PH, Lin HTV (2019) Repeated-batch lactic acid fermentation using a novel bacterial immobilization technique based on a microtube array membrane. Process Biochem 20:20

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Paper and Bioprocess Engineering, SUNY College of Environmental Science and Forestry, Syracuse, NY, 13210, USA

    Jianfei Wang, Huanyu Guo, Shaoming Jiang, Jing Zhang, Yuchen Ning, Mudannan Fang & Shijie Liu

  2. The Center for Biotechnology and Interdisciplinary Studies (CBIS) at Rensselaer Polytechnic Institute, Troy, NY, 12180, USA

    Jiaqi Huang

Authors
  1. Jianfei Wang
    View author publications

    Search author on:PubMed Google Scholar

  2. Jiaqi Huang
    View author publications

    Search author on:PubMed Google Scholar

  3. Huanyu Guo
    View author publications

    Search author on:PubMed Google Scholar

  4. Shaoming Jiang
    View author publications

    Search author on:PubMed Google Scholar

  5. Jing Zhang
    View author publications

    Search author on:PubMed Google Scholar

  6. Yuchen Ning
    View author publications

    Search author on:PubMed Google Scholar

  7. Mudannan Fang
    View author publications

    Search author on:PubMed Google Scholar

  8. Shijie Liu
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to Shijie Liu.

Ethics declarations

Conflict of interest

The authors declare that they have no potential conflict of interest.

Ethical standards

The contents in this paper are the results of independent research. All data and pictures are authentic and reliable. In addition to the references already indicated in the text, the research results of this paper do not include any content of copyright enjoyed by others. Other individuals and groups contributing to the research work involved in this paper have been clearly identified in this paper. This research does not involve human participants or animals. The publication of the paper has been agreed by the authors and does not involve potential conflicts of interest. I am fully aware of the legal liability of this statement.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, J., Huang, J., Guo, H. et al. Optimization of immobilization conditions for Lactobacillus pentosus cells. Bioprocess Biosyst Eng 43, 1071–1079 (2020). https://doi.org/10.1007/s00449-020-02305-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00449-020-02305-9

Keywords