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An auto-inducible expression and high cell density fermentation of Beefy Meaty Peptide with Bacillus subtilis

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Abstract

Currently, some cases about the expression of flavor peptides with microorganisms were reported owing to the obvious advantages of biological expression over traditional methods. However, beefy meaty peptide (BMP), the focus of umami peptides, has neither been concerned in its safe expression nor its overproduction in fermenter. In this study, multi-copy BMP (8BMP) was successfully auto-inducibly expressed and efficiently produced in Bacillus subtilis 168. First, 8BMP was successfully auto-inducibly expressed with srfA promoter in B. subtilis 168. Further, the efficient production of 8BMP was researched in a 5-L fermenter: the fermentation optimized by Pontryagin’s maximum principle obtained the highest 8BMP yield (3.16 g/L), which was 1.2 times and 1.8 times than that of two-stage feeding cultivation (2.67 g/L) and constant-rate feeding cultivation (1.75 g/L), respectively. Overall, the auto-inducible expression of 8BMP in B. subtilis and fermentation with Pontryagin’s maximum principle are conductive for overproduction of BMP and other peptides.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31601418, 31601444 and 21506172), the School level project of Hengyang Normal University (18D14), the Applied Basic Research Programs of Science and Technology Department of Sichuan Province (2019YJ0390), the Spring Sunshine Plan of the Ministry of Education of China (Z2015121), the Key Project of Xihua University of China (z1420524 and z1420525), the Department of Education of Sichuan Province of China (15205445), the Key Laboratory program of Food and Biotechnology of Xihua University of China (szjj2015-013 and szjj2015-014). Undergraduate Training Program for Innovation (201510623003 and 201510623053).

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Authors and Affiliations

  1. College of Life Science and Environment, Hengyang Normal University, Hengyang, 421008, China

    Weifeng Sun, Yi Liu & Xiaohua Chen

  2. Key Laboratory of Food and Biotechnology, School of Food and Biotechnology, Xihua University, Chengdu, 610039, China

    Yuanming Wu, Wenwu Ding, Li Wang, Lunjie Wu, Lu Lin & Zhenming Che

  3. School of Biochemical Engineering, Anhui Polytechnic University, Anhui, 241000, China

    Longbao Zhu

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  1. Weifeng Sun
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Sun, W., Wu, Y., Ding, W. et al. An auto-inducible expression and high cell density fermentation of Beefy Meaty Peptide with Bacillus subtilis. Bioprocess Biosyst Eng 43, 701–710 (2020). https://doi.org/10.1007/s00449-019-02268-6

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