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Production of d-allose from d-fructose using immobilized l-rhamnose isomerase and d-psicose 3-epimerase

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Abstract

d-Allose is a rare sugar, can be used as an ingredient in a range of foods and dietary supplements, has alimentary activities, especially excellent anti-cancer effects and used in assisting cancer chemotherapy and radiotherapy, etc. To develop a simple and low-cost process for d-allose production, a one-pot enzymatic process using the substrate of d-fructose, and the recombinant enzymes of d-psicose 3-epimerase (DPE) and l-rhamnose isomerase (l-RhI) was developed. These enzymes were cloned from Ruminococcus sp. and B. subtilis, respectively, successfully expressed in E. coli, extracted and immobilized using anion exchange resin and amino resin, respectively. The mass ratio of d-fructose, d-psicose and d-allose was 6.6:2.4:1.0 when the reaction reached equilibrium after 5 h of reaction. Using the low-cost substrate of d-fructose, the reusable immobilized enzymes and the one-pot reaction, the production process is simplified and the production cost is decreased. In addition, to simplify the enzyme extraction and immobilization processes, new methods for enzyme capture and immobilization were developed especially for DPE immobilization. This is the first report for one-pot d-allose production using immobilized l-RhI and DPE.

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Acknowledgements

This research was funded by the Key R & D Plan of Shandong Province in 2019 (2019GSF107015), and Shandong Province Science and Technology Development Project (2015GSF121016) of China.

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Author notes

  1. Can Li and Jianqiang Lin contributed equally to this work.

Authors and Affiliations

  1. School of Bioengineering, Qi Lu University of Technology, Jinan, 250353, People’s Republic of China

    Can Li

  2. State Key Laboratory of Microbial Technology, Microbiology Technology Institute, Shandong University, Qingdao, People’s Republic of China

    Kai Du, Jianqun Lin & Jianqiang Lin

  3. Institute of Information Science and Engineering, School of Information Science and Engineering, Shandong Normal University, Jinan, People’s Republic of China

    Ling Gao

  4. Shandong Academy of Chinese Medicine, Jinan, 250014, People’s Republic of China

    Huibin Lin

  5. Qingdao Longding Biotech Limited Company, Qingdao, People’s Republic of China

    Yilin Ren

Authors
  1. Can Li
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  2. Ling Gao
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  3. Kai Du
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  4. Huibin Lin
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  5. Yilin Ren
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  6. Jianqun Lin
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  7. Jianqiang Lin
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Contributions

CL and JL designed this study; CL and JL conducted the experiments; CL, LG, KD, HL, JL, and JL analyzed the data; CL and JL wrote the paper; YR revised the manuscript. All the authors have read the manuscript critically.

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Correspondence to Jianqun Lin or Jianqiang Lin.

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The authors declare that there are no studies conducted with human participants or animals.

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Li, C., Gao, L., Du, K. et al. Production of d-allose from d-fructose using immobilized l-rhamnose isomerase and d-psicose 3-epimerase. Bioprocess Biosyst Eng 43, 645–653 (2020). https://doi.org/10.1007/s00449-019-02262-y

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  • DOI: https://doi.org/10.1007/s00449-019-02262-y

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