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A versatile Pseudomonas putida KT2440 with new ability: selective oxidation of 5-hydroxymethylfurfural to 5-hydroxymethyl-2-furancarboxylic acid

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Abstract

Currently, biotransformation of 5-hydroxymethylfurfural (HMF) into a series of high-value bio-based platform chemicals is massively studied. In this study, selective biooxidation of HMF to 5-hydroxymethyl-2-furancarboxylic acid (HMFCA) by Pseudomonas putida KT2440 with superior titer, yield, and productivity was reported. The biocatalytic performances of P. putida KT2440 were optimized separately. Under optimal conditions, 100% yield of HMFCA was obtained when HMF concentration was less than 150 mM, while the maximum concentration of 155 mM was achieved from 160 mM HMF in 12 h. P. putida KT2440 was highly tolerate to HMF, up to 190 mM. Besides, it was capable of selective oxidation of other furan aldehydes to the corresponding carboxylic acids with good yield of 100%. This study further demonstrates the potential of P. putida KT2440 as a biocatalyst for biomass conversion, as this strain has been proved the capacity to convert and utilize many kinds of biomass-derived sugars and ligin-derived aromatic compounds.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (51776099).

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Author notes

  1. Qianqian Xu and Zhaojuan Zheng contributed equally to this work.

Authors and Affiliations

  1. Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, People’s Republic of China

    Qianqian Xu, Zhaojuan Zheng, Lihua Zou, Chen Zhang & Jia Ouyang

  2. College of Forestry, Nanjing Forestry University, Nanjing, 210037, People’s Republic of China

    Qianqian Xu

  3. College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, People’s Republic of China

    Zhaojuan Zheng, Lihua Zou, Chen Zhang, Fen Yang, Kaijie Zhou & Jia Ouyang

  4. Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing, 210037, People’s Republic of China

    Zhaojuan Zheng & Jia Ouyang

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  1. Qianqian Xu
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  2. Zhaojuan Zheng
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  3. Lihua Zou
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  4. Chen Zhang
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  5. Fen Yang
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  6. Kaijie Zhou
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  7. Jia Ouyang
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Correspondence to Jia Ouyang.

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Xu, Q., Zheng, Z., Zou, L. et al. A versatile Pseudomonas putida KT2440 with new ability: selective oxidation of 5-hydroxymethylfurfural to 5-hydroxymethyl-2-furancarboxylic acid. Bioprocess Biosyst Eng 43, 67–73 (2020). https://doi.org/10.1007/s00449-019-02205-7

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  • DOI: https://doi.org/10.1007/s00449-019-02205-7

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