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Exploring encapsulation strategies as a protective mechanism to avoid amensalism in mixed populations of Pseudomonas taetrolens and Lactobacillus casei

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Abstract

Pseudomonas taetrolens constitutes an efficient platform for the biosynthesis of lactobionic acid, a potentially prebiotic compound. Unfortunately, an amensalistic interaction has been demonstrated between P. taetrolens and probiotic lactic acid bacteria (LAB), characterized by the competitive exclusion of P. taetrolens, hindering the in situ production of fermented dairy products with synbiotic properties. In the present research, encapsulation was explored as a barrier to the diffusion of the antimicrobial metabolites generated by LAB. Mixed fermentations involving P. taetrolens LMG 2336 and Lactobacillus casei CECT 475 were cultivated, entrapping both microorganisms alternately. Alginate, alginate/starch and carboxymethyl cellulose/k-carrageenan were tested as encapsulating agents. The immobilization of L. casei in 2% alginate/2% starch beads was found to be the best strategy, improving the production of lactobionic acid by 182% with respect to co-cultures with free cells. This study proves the potential of LAB encapsulation for the protection of sensitive strains in mixed food fermentations.

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Acknowledgements

Authors thank the financial support from the Government of the Principality of Asturias by the project FC-GRUPIN-IDI/2018/000127. Authors also wish to thank the technical assistance of Marta Alonso (Process Image Area, Scientific-Technical Services, University of Oviedo).

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Authors and Affiliations

  1. Department of Chemical and Environmental Engineering, University of Oviedo, Faculty of Chemistry, C/Julián Clavería S/N, 33071, Oviedo, Spain

    Cristina García, Giuliana Ranieri, Manuel Rendueles & Mario Díaz

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  1. Cristina García
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  2. Giuliana Ranieri
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  3. Manuel Rendueles
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  4. Mario Díaz
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Correspondence to Mario Díaz.

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García, C., Ranieri, G., Rendueles, M. et al. Exploring encapsulation strategies as a protective mechanism to avoid amensalism in mixed populations of Pseudomonas taetrolens and Lactobacillus casei. Bioprocess Biosyst Eng 43, 55–66 (2020). https://doi.org/10.1007/s00449-019-02204-8

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  • DOI: https://doi.org/10.1007/s00449-019-02204-8

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