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Production of metabolites with antioxidant activity by Botryosphaeria dothidea in submerged fermentation

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Abstract

Antioxidants are able of inhibiting free radicals and play an important role in human diet, protection of foods, anti-aging cosmetics, among others. Fungi have been highlighted in production of metabolites with biological activity, such as antioxidant. The main objective of this study was to select a fungus from resources of Brazilians Pampa and Atlantic Forest biomes with hability for production of antioxidants by submerged fermentation. The scavenging activity of the compounds was determined against radical 2.2-diphenyl-1-picrylhydrazyl (DPPH), superoxide and hydroxyl. Botryosphaeria dothidea exhibited higher potential for the production of antioxidants, and its supernatant showed percentage inhibition values of 94.47, 94.87 and 89.78% against DPPH, superoxide and hydroxyl radicals and IC50 of 0.206 mg/mL. The identification of the volatile compounds present in the broth verified the presence of the hexahydropyrrolizin-3-one; 1.2-benzenedicarboxylic acid bis(2-methylpropyl) ester and 3.6-bis(2-methylpropyl)-2.5-piperazinedione. According to this study, Botryosphaeria dothidea presents great potential to produce compounds with antioxidant activity.

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Acknowledgements

The authors would like to thank to CAPES (Coordination for the Improvement of Higher Education Personnel) by the scholarship for the author S. P. D.

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Authors and Affiliations

  1. Department of Chemical Engineering, Federal University of Santa Maria, Av.Roraima, 1000, Santa Maria, RS, 97105-900, Brazil

    Susanne Pedroso Druzian, Luíza Negri Pinheiro, Nathália Marques Bastos Susin, Marcio Antonio Mazutti, Raquel Cristine Kuhn & Lisiane de Marsillac Terra

  2. Department of Chemistry, Federal University of Santa Maria, Av.Roraima, 1000, Santa Maria, RS, 97105-900, Brazil

    Valéria Dal Prá

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  1. Susanne Pedroso Druzian
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  2. Luíza Negri Pinheiro
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Correspondence to Susanne Pedroso Druzian.

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Druzian, S.P., Pinheiro, L.N., Susin, N.M.B. et al. Production of metabolites with antioxidant activity by Botryosphaeria dothidea in submerged fermentation. Bioprocess Biosyst Eng 43, 13–20 (2020). https://doi.org/10.1007/s00449-019-02200-y

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