There is a need to develop low operational intensity, cost-effective, and small-footprint systems to treat wastewater. Partial nitritation has been studied using a variety of control strategies, however, a gap in passive operation is evident. This research investigates the use of elevated loading rates as a strategy for achieving low operational intensity partial nitritation in a moving bed biofilm reactor (MBBR) system. The effects of loading rates on nitrification kinetics and biofilm characteristics were determined at elevated, steady dissolved oxygen concentrations between 5.5 and 7.0 mg O2/L and ambient temperatures between 19 and 21 °C. Four elevated loading rates (3, 4, 5 and 6.5 g NH4+-N/m2 days) were tested with a distinct shift in kinetics being observed towards nitritation at elevated loadings. Complete partial nitritation (100% nitrite production) was achieved at 6.5 g NH4+-N/m2 days, likely due to thick biofilm (572 µm) and elevated NH4+-N load, which resulted in suppression of nitrite oxidation.
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US Clean Water Act, 33 U.S.C. § 1251 et seq. 1972 (2018) US Environmental Protections Agency, Washington. https://www.epa.gov/laws-regulations/summary-clean-water-act. Accessed Feb 2018
Council Directive 91/271/EEC of 27 May 1991 concerning urban waste water treatment (2018) https://www.ecolex.org/details/legislation/council-directive-91271eec-concerning-urban-waste-water-treatment-lex-faoc013224. Accessed May 2018
Canadian Fisheries Act: Wastewater Systems Effluent Regulations (2012) Canada Gazetter Part II, 146(15). Retrieved from the Canada Gazette website: https://www.gazette.gc.ca/rp-pr/p2/2012/2012-07-18/html/sor-dors139-eng.html. Accessed May 2018
World Resources Institute (2018) https://www.wri.org/our-work/project/eutrophication-and-hypoxia/sources-eutrophication. Accessed May 2018
Delatolla R, Babarutsi S (2005) Parameters effecting hydraulic behaviour of aerated lagoons. J Environ Eng 131(10):1404–1413
Mulder A, vandeGraaf AA, Robertson LA, Kuenan JG (1995) Anaerobic ammonium oxidation discovered in a denitrifying fluidized bed reactor. FEMS Microbiol Ecol 16:177–184
Strous M, Heijnen JJ, Kuenen JG, Jetten MSM (1998) The sequencing batch reactor as a powerful toll to study very slowly growing micro-organisms. Appl Microbiol Biotechnol 50:589–596
Lackner S, Gilbert EM, Vlaeminck SE, Joss A, Horn H, van Loosdrecht MCM (2014) Full-scale partial nitritation/anammox experiences—an application survey. Water Res 55:292–303
van Dongen U, Jetten MSM, van Loosdrecht MCM (2001) The SHARON-Anammox process for treatment of ammonium rich wastewater. Water Sci Technol 44:153–160
Rosenwinkel K, Cornelius A (2005) Deammonification in the moving-bed process for the treatment of wastewater with high ammonia content. Chem Eng Technol 28:49–52
Wett B (2007) Development and implementation of a robust deammonification process. Water Sci Tech 56:81–88
Christensson M, Ekström S, Chan AA, Le Vaillant E, Lemarie R (2013) Experience from start-ups of the first ANITA Mox plants. Water Sci Tech 67:2677–2684
Hellinga C, Schellen AAJC, Mulder JW, van Loosdrecht MCM, Heijnen JJ (1998) The SHARON process: an innovative method for nitrogen removal from ammonium-rich wastewater. Water Sci Tech 37:135–142
Fux C, Huang D, Monti A, Siegrist H (2004) Difficulties in maintaining long-term partial nitritation of ammonium-rich sludge digester liquids in a moving-bed biofilm reactor (MBBR). Water Sci Tech 49:53–60
Brockmann D, Morgenroth E (2010) Evaluating operating conditions for outcompeting nitrite oxidizers and maintaining partial nitrification in biofilm systems using biofilm modeling and Monte Carlo filtering. Water Res 44(6):1995–2009
Park S, Wookeun B, Rittmann BE (2010) Operational boundaries for nitrite accumulation in nitrification based on minimum/maximum substrate concentrations that include effects of oxygen limitation, pH, and Free Ammonia and Free Nitrous acid inhibition. Envrion Sci Technol 44:335–342
Park S, Chung J, Rittmann BE, Wookeun B (2014) Nitrite accumulation from simultaneous free-ammonia and free-nitrous-acid inhibition and oxygen limitation in a continuous flow biofilm reactor. Biotechnol Bioeng 112(1):43–52
Gaul T, Märker S, Kunst S (2005) Start-up of moving bed biofilm reactors for deammonification: the role of hydraulic retention time, alkalinity and oxygen supply. Water Sci Technol 52(7):127–133
Bartroli A, Pérez J, Carrera J (2010) Applying ratio control in a continuous granular reactor to achieve full nitritation under stable operating conditions. Envrion Sci Technol 44:8930–8935
Wang R, Terada A, Lackner S, Smets BF, Henze M, Xia S, Zhao J (2009) Nitritation performance and biofilm development of co-and counter—diffusion biofilm reactors: modeling and experimental comparison. Water Res 43(10):2699–2709
Huiliñir C, Romero R, Muñoz C, Bornhardt C, Roeckel M, Antileo C (2010) Dynamic modeling of partial nitrification in a rotating disk biofilm reactor: Calibration, validation and simulation. Biochem Eng J 52(1):7–18
Hao X, Heijnen JJ, van Loosedrecht MCM (2002) Sensitivity analysis of a biofilm model describing a one-stage completely autotrophic nitrogen removal (Canon) process. Biotechnol Bioeng 77:266–277
Gilbert EM, Agrawal S, Schwartz T, Horn H, Lackner S (2015) Comparing different reactor configurations for partial nitritation/anammox at low temperatures. Water Res 81:92–100
Piculell M, Christensson M, Jönsson K, Welander T (2016) Partial nitrification in MBBRs for mainstream deammonification with thin biofilms and alternating feed supply. Water Sci Tech 73:1253–1260
Laureni M, Falâs P, Robin O, Wick A, Weissbrodt DG, Nielsen JL, Ternes TA, Morgenroth E, Joss A (2016) Mainstream partial nitritation and anammox: long-term process stability and effluent quality at low temperatures. Water Res 101:628–639
Isanta E, Reino C, Carrera J, Pérez J (2015) Stable partial nitritation for low-strength wastewater at low temperature in an aerobic granular reactor. Water Res 80(1):149–158
Bian W, Zhang S, Zhang Y, Li W, Kan R, Wang W, Zheng Z, Li J (2017) Achieving nitritation in a continuous moving bed biofilm reactor at different temperatures through ratio control. Biores Technol 226:73–79
Peng Y, Gao S, Wang S, Bai L (2007) Partial nitrification from domestic wastewater by aeration control at ambient temperature. Chin J Chem Eng 15:115–121
Durián U, del Val Rio A, Campos JL, Mosquera-Corral A, Méndez R (2014) Enhanced ammonia removal at room temperature by pH controlled partial nitrification and subsequent anaerobic ammonium oxidation. Environ Technol 35:383–390
Ganigué R, Gaborró J, Sànchez-Melsió A, Ruscalleda M, López H, Vila X, Colprim J, Balaguer MD (2009) Long-term operation of a partial nitritation pilot plant treating leachate with extremely high ammonium concentration prior to an anammox process. Biores Technol 100(23):5624–5632
Zhang L, Yang J, Hira D, Fuji T, Furukawa K (2011) High-rate partial nitrification treatment of reject water as a pretreatment for anaerobic ammonium oxidation (anammox). Biores Technol 102(4):3761–3767
Garrido JM, van Benthum WAJ, van Loosdretcht MCM, Heijnen JJ (1997) Influence of dissolved oxygen concentration on nitrite accumulation in a biofilm airlift suspension reactor. Biotechnol Bioeng 53(2):168–178. https://doi.org/10.1002/(sici)1097-0290(19970120)53:2%3c168:aid-bit6%3e3.0.co;2-m
Li Y, Wang Z, Li J, Wei J, Zhang Y, Zhao B (2017) Inhibition kinetics of nitritation and half-nitritation of old landfill leachate in a membrane bioreactor. J Biosci Bioeng 123(4):482–488
Zheng Z, Li Z, Ma J, Chen G, Bian W, Li J, Zhao B (2016) The nitritation performance of biofilm reactor for treating domestic wastewater under high dissolved oxygen. J Environ Sci 42:267–274
Delatolla R, Tufenkji N, Comeau Y, Gadbois A, Lamarre D, Berk D (2009) Kinetic analysis of attached growth nitrification in cold climates. Water Sci Tech 60:1173–1184
Hoang V, Delatolla R, Laflamme E, Gadbois A (2014) An investigation of moving bed biofilm reactor nitrification during long-term exposure to cold temperatures. Water Environ Res 86:36–42
Tian X, Ahmed W, Delatolla R (2017) Nitrifying bio-cord reactor: performance optimization and effects of substratum and air scouring. Environ Technol 1:1. https://doi.org/10.1080/09593330.2017.1397760
APHA, AWWA, WEF (1989) Standard methods for examination of water and wastewater. Washington, DC, 17th ed
Delatolla R, Séguin C, Springthorpe S, Gorman E, Campbell A, Douglas I (2015) Disinfection byproduct formation during biofiltration cycle: implications for drinking water production. Chemosphere 136:190–197
Delatolla R, Tufenkji N, Comeau Y, Gadbois A, Lamarre D, Berk D (2012) Effects of long exposure to low temperatures on nitrifying biofilm and biomass in wastewater treatment. Water Environ Res 84:328–338
Young B, Delatolla R, Ren B, Kennedy K, Laflamme E, Stintzi A (2016) Pilot-scale tertiary MBBR nitrification at 1 °C: characterization of ammonia removal rate, solids settleability and biofilm characteristics. Environ Technol 37:2124–2132
Young B, Delatolla R, Kennedy K, Laflamme E, Stintzi A (2017) Low temperature MBBR nitrification: microbiome analysis. Water Res 111:224–233
Delatolla R, Berk D, Tufenkji N (2008) Rapid and reliable quantification of biofilm weight and nitrogen content of biofilm attached to polystyrene beads. Water Res 42:3082–3088
Forrest D, Delatolla R, Kennedy K (2016) Carrier effects on tertiary nitrifying moving bed biofilm reactor: an examination of performance, biofilm and biologically produced solids. Environ Technol 37:662–671
Young B, Delatolla R, Abujamel T, Kennedy K, Laflamme E, Stintzi A (2017) Rapid start-up of nitrifying MBBRs at low temperatures: nitrification, biofilm response and microbiome analysis. Bioprocess Biosyst Eng 40:731–739
Ødegaard H (1999) The moving bed biofilm reactor. Water environmental engineering and reuse of water. Hokkaido Press, Hokkaido, pp 250–305
Ciesielski S, Dorota K, Ewelina K, Przenyslaw K (2010) Characterization of bacterial structures in a two-stage moving bed biofilm reactor (MBBR) during nitrification of landfill leachate. J Microbiol Biotechnol 20:1140–1151
Brockmann D, Morgenroth E (2008) Partial nitrification in biofilms: inhibition versus competition. In: Outline paper for IWA world water congress
Bryers JD (1987) Biologically active surfaces: processes governing the formation and persistence of biofilms. Biotechnol Prog 3:57–68
Flemming HC (1993) Biofilms and environmental protection. Water Sci Tech 27:1–10
Beer DD, Stoodley P, Roe F, Lewandowski Z (1994) Effects of biofilm structures on oxygen distribution and mass transport. Biotechnol Bioeng 43:1131–1138
White C, Gadd GM (2000) Copper accumulation by sulfate reducing bacterial biofilms. FEMS Microbiol Lett 183:313–318
Bjornberg C, Lin W, Zimmerman RA (2009) Effect of temperature on biofilm growth dynamics and nitrification in a full-scale MBBR system. In: Proceedings of the Water Environment Federation, WEFTEC: Session 61 through 70, pp 4407–4426
Duan H, Ye L, Lu X, Yuan Z (2019) Overcoming nitrite oxidizing bacteria adaptation through alternating sludge treatment with free nitrous acid and free ammonia. Sci Technol Environ. https://doi.org/10.1021/acs.est.8b06148
The authors acknowledge the NSERC CREATE TECHNOMISE program for their contributions as well as Veolia Water Technologies Canada for technical support and in kind donations.
This study was partially funded by the Natural Science and Engineering Research Council of Canada (NSERC) Discovery Grant and NSERC CREATE grant in Technologies for Microbiome Science and Engineering (TECHNOMISE CREATE 497995-2017).
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Schopf, A., Delatolla, R. & Kirkwood, K.M. Partial nitritation at elevated loading rates: design curves and biofilm characteristics. Bioprocess Biosyst Eng 42, 1809–1818 (2019). https://doi.org/10.1007/s00449-019-02177-8
- Partial nitritation
- Surface area loading rate
- Moving bed biofilm reactor
- Small footprint