Bioprocess and Biosystems Engineering

, Volume 42, Issue 8, pp 1375–1384 | Cite as

Soybean processing wastewater supported the removal of propyzamide and biochemical accumulation from wastewater by Rhodopseudomonas capsulata

  • Pan Wu
  • Ziqiao  Han
  • Wentao  Mo
  • Xiaozhen  Wu
  • Zhaobo ChenEmail author
  • Ying ZhangEmail author
  • Yanling Wang
  • Yubo Cui
  • Yuying Dong
  • Hongjie Sun
  • Xuejun Zou
Research Paper


Simultaneous (SPW and propyzamide) wastewater treatment and the production of biochemicals by Rhodopseudomonas capsulata (R. capsulata) were investigated with supplement of soybean processing wastewater (SPW). Compared to control group, propyzamide was removed and biochemicals production were enhanced with the supplement of SPW. Propyzamide induced camH gene expression through activating MAPKKKs gene in MAPK signal transduction pathway. The induction of camH gene and CamH occurs after 1 day for R. capsulata. However, lack of organics in original wastewater did not maintain R. capsulata growth for over 1 day. The supplement of SPW provided sufficient carbon source for R. capsulata under three addition dosages. This new method resulted in the mixed (SPW and propyzamide) wastewater treatment and improvement of biochemicals simultaneously, as well as the realization of reutilization of wastewater and R. capsulata as sludge. Meanwhile, high-order nonlinear mathematical model of the relationship between propyzamide removal rate, Xt and Xt/r, was established.


Propyzamide wastewater CamH camH gene SPW R. capsulata 



The authors gratefully acknowledge the National Nature Science Fund for Distinguished Young Scholars of China (Grant no. 41625002), the National Natural Science Foundation Young of China (Grant no. 31700432), the National Natural Science Foundation of China (Grant nos. 31700432; 31470550; 81500493; 31400386; 51778114), Post-doctoral Science Foundation Project of China and Heilongjiang Province of China (Grant nos. 2018M641797; LBH-Z18025), Natural Science Foundation of Guangdong Province (Grant nos. 2015A030313098), Application Technology Research and Development Projects of Harbin (Grant no. 2016RAXXJ103), and the MOA Modern Agricultural Talents Support Project for valuable financial support, and Basic scientific research service fees of the Central University and Dalian Nationalities University (0113-20000101).

Supplementary material

449_2019_2137_MOESM1_ESM.docx (105 kb)
Supplementary material 1 (DOCX 104 KB)


  1. 1.
    Khan MA, Brown CD (2017) Influence of commercial formulation on the sorption and leaching behaviour of propyzamide in soil. Sci Total Environ 578:158–166CrossRefGoogle Scholar
  2. 2.
    Zhang Y, Pan JH, Zhang GW, Zhou XY (2015) Intercalation of herbicide propyzamide into DNA using acridine orange as a fluorescence probe. Sens Actuators B Chem 206:630–639CrossRefGoogle Scholar
  3. 3.
    Wu P, Chen ZB, Zhang Y, Wang YL, Zhu FF, Cao B, Wu Y, Li N (2019) Rhodopseudomonas palustris wastewater treatment: cyhalofop-butyl removal, biochemicals production and mathematical model establishment. Bioresource Tech 282:390–397CrossRefGoogle Scholar
  4. 4.
    Jiang Z, Zhang XY, Wang ZY, Cao B, Deng SJ, Bi MC, Zhang Y (2019) Enhanced biodegradation of atrazine by Arthrobacter sp. DNS10 during co-culture with a phosphorus solubilizing bacteria: Enterobacter sp. P1. Ecotoxicol Environ Safety 17:159–166CrossRefGoogle Scholar
  5. 5.
    Wu P, Cao B, Zhang Y, Li WB, Wang YL, Wu Y, Li N (2019) The bio-mitigation of acetochlor in soil using Rhodopseudomonas capsulata in effluent after wastewater treatment. J Soils Sediments 2:1–7Google Scholar
  6. 6.
    Wu P, Zhang Y, Chen ZB, Wang YL, Zhu FF, Cao B, Wu Y, Li N (2019) The organophosphorus pesticides in soil was degradated by Rhodobacter sphaeroides after wastewater treatment. Biochem Eng J 141:247–251CrossRefGoogle Scholar
  7. 7.
    Puyol D, Barry EM, Hülsen T, Batstone DJ (2017) A mechanistic model for anaerobic phototrophs in domestic wastewater applications: photo-anaerobic model (PAnM). Water Res 116:241–253CrossRefGoogle Scholar
  8. 8.
    Ponsano EHG, Paulino CZ, Pinto MF (2008) Phototrophic growth of Rhodobacter sphaeroides in poultry slaughterhouse wastewater. Bioresour Technol 99:3836–3842CrossRefGoogle Scholar
  9. 9.
    Wu P, Li JZ, Wang YL, Liu XS, Du C, Tong QY, Li N (2014) Promoting the growth of Rhodobacter sphaeroides in sewage purification by addition of magnesium ions. Biochem Eng J 91:66–71CrossRefGoogle Scholar
  10. 10.
    Kong FY, Wang AJ, Ren HY, Huang LP, Xu MY, Tao HC (2014) Improved dechlorination and mineralization of 4-chlorophenol in a sequential biocathode–bioanode bioelectrochemical system with mixed photosynthetic bacteria. Bioresour Technol 158:32–38CrossRefGoogle Scholar
  11. 11.
    Ouchane S, Picaud M, Astier C (1995) A new mutation in the pufL gene responsible for the terbutryn resistance phenotype in Rhodobacter sphaeroides. FEBS Lett 374(1):130–134CrossRefGoogle Scholar
  12. 12.
    Sasikala C, Ramana CV (1997) Biodegradation and Metabolism of unusual carbon compounds by anoxygenic phototrophic bacteria. Adv Microb Physiol 39:339–377CrossRefGoogle Scholar
  13. 13.
    Yu HQ, Wilson F, Tay J (1998) Kinetic analysis of an anaerobic filter treating starch sewage. Water Res 32:3341–3352CrossRefGoogle Scholar
  14. 14.
    Giotta L, Agostiano A, Italiano F, Milano F, Trotta M (2006) Heavy metal ion influence on the photosynthetic growth of Rhodobacter sphaeroides. Chemosphere 62:1490–1499CrossRefGoogle Scholar
  15. 15.
    Italiano F, Buccolieri A, Giotta L, Agostiano A, Valli L, Milano F, Trotta M (2009) Response of the carotenoidless mutant Rhodobacter sphaeroides growing cells to cobalt and nickel exposure. Int Biodeterior Biodegrad 63:948–957CrossRefGoogle Scholar
  16. 16.
    Takeda N, Iwata N, Torimoto T, Yoneyama H (1998) Influence of carbon black as an adsorbent used in TiO2 photocatalyst films on photodegradation behaviors of propyzamide. J Catal 177(2):240–246CrossRefGoogle Scholar
  17. 17.
    Zhao BP, Hua XD, Wang F, Dong WL, Wang MH (2015) Biodegradation of propyzamide by Comamonas testosteroni W1 and cloning of the propyzamide hydrolase gene camH. Bioresour Technol 179:144–149CrossRefGoogle Scholar
  18. 18.
    Beta T, Hwang T (2018) Influence of heat and moisture treatment on carotenoids, phenolic content, and antioxidant capacity of orange maize flour. Food Chem 246:58–64CrossRefGoogle Scholar
  19. 19.
    Rasouli Z, Valverde-Pérez B, Este MD, Francisci DD, Angelidaki I (2018) Nutrient recovery from industrial wastewater as single cell protein by a co-culture of green microalgae and methanotrophs. Biochem Eng J 134(15):129–135CrossRefGoogle Scholar
  20. 20.
    Rudolf C, Grammel H (2012) Fructose metabolism of the purple non-sulfur bacterium Rhodospirillum rubrum: effect of carbon dioxide on growth, and production of bacteriochlorophyll and organic acids. Enzyme Microb Technol 50(4–5):238–246CrossRefGoogle Scholar
  21. 21.
    Qin W, Deng T, Cui HY. Zhang Q, Liu XD, Yang X, Chen MQ (2018) Exposure to diisodecyl phthalate exacerbated Th2 and Th17-mediated asthma through aggravating oxidative stress and the activation of p38 MAPK. Food Chem Toxicol 114:78–87CrossRefGoogle Scholar
  22. 22.
    Raja V, Majeed U, Kang HS, Andrabi KI, John R (2017) Abiotic stress: interplay between ROS, hormones and MAPKs. Environ Exp Bot 137:142–157CrossRefGoogle Scholar
  23. 23.
    Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta DeltaC(T)). Method Methods 25:402–408CrossRefGoogle Scholar
  24. 24.
    Heger J, Wiltafsky M, Zelenka J (2016) Impact of different processing of full-fat soybeans on broiler performance. Czech J Anim Sci 61:57–66CrossRefGoogle Scholar
  25. 25.
    Nahavandinejad M, Seidavi AR, Asadpour L (2012) Effects of soybean meal processing method on the broiler immune system. Kafkas Univer Veteriner Fakultesi Dergisi 18(6):965–972Google Scholar
  26. 26.
    Tousi Mojarrad M, Seidavi AR, Dadashbeiki M, Roca-Fernandez AI (2014) Effect of soybean meal heat procedures on growth performance of broiler chickens. Span J Agric Res 12(1):180–185CrossRefGoogle Scholar
  27. 27.
    Nahavandinejad M, Seidavi AR, Asadpour L, Payan-Carreira R (2014) Blood biochemical parameters of broilers fed differently thermal processed soybean meal. Rev MVZ Cordoba 19(3):4301–4315CrossRefGoogle Scholar
  28. 28.
    Jahanian R, Rasouli E (2016) Effect of extrusion processing of soybean meal on ileal amino acid digestibility and growth performance of broiler chicks. Poult Sci 95(12):2871–2878CrossRefGoogle Scholar
  29. 29.
    Fenga C, Gangemi S, Giambò F, Tsitsimpikou C (2016) Low-dose occupational exposure to benzene and signal transduction pathways involved in the regulation of cellular response to oxidative stress. Life Sci 147(15):67–70CrossRefGoogle Scholar
  30. 30.
    Hendrix S, Schröder P, Keunen E, Huber C, Cuypers A (2017) Chapter six: molecular and cellular aspects of contaminant toxicity in plants: the importance of sulphur and associated signalling pathways. Adv Bot Res 83:223–276CrossRefGoogle Scholar
  31. 31.
    Zhou Q, Zhang PY, Zhang GM (2015) Biomass and pigments production in photosynthetic bacteria wastewater treatment: effects of light sources. Bioresour Technol 179:505–509CrossRefGoogle Scholar
  32. 32.
    Zheng YX, Wang YL, Pan J, Zhang JR, Chen KY (2017) Semi-continuous production of high-activity pectinases by immobilized Rhizopus oryzae using tobacco wastewater as substrate and their utilization in the hydrolysis of pectin-containing lignocellulosic biomass at high solid content. Bioresour Technol 241:1138–1144CrossRefGoogle Scholar
  33. 33.
    Ma JX, Wang ZW, Zhang JY, Waite TD, Wu ZC (2017) Cost-effective Chlorella biomass production from dilute wastewater using a novel photosynthetic microbial fuel cell (PMFC). Water Res 108:356–364CrossRefGoogle Scholar
  34. 34.
    Libardi N, Soccol CR, Góes-Neto A, Oliveira JD, Vandenberghe LPDS (2017) Domestic wastewater as substrate for cellulase production by Trichoderma harzianum. Process Biochem 57:190–199CrossRefGoogle Scholar
  35. 35.
    Chen BL, Wan C, Mehmood MA, Chang JS, Zhao XQ (2017) Manipulating environmental stresses and stress tolerance of microalgae for enhanced production of lipids and value-added products—a review. Bioresour Technol 244:1198–1206CrossRefGoogle Scholar
  36. 36.
    Lee NY, Ko SR, Ahn CY, Oh HM (2018) Optimized co-production of lipids and carotenoids from Ettlia sp. by regulating stress conditions. Bioresour Technol 258:234–239CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Pan Wu
    • 1
    • 2
  • Ziqiao  Han
    • 1
  • Wentao  Mo
    • 1
  • Xiaozhen  Wu
    • 1
  • Zhaobo Chen
    • 1
    • 2
    Email author
  • Ying Zhang
    • 1
    • 2
    Email author
  • Yanling Wang
    • 3
  • Yubo Cui
    • 1
  • Yuying Dong
    • 1
    • 2
  • Hongjie Sun
    • 1
    • 2
  • Xuejun Zou
    • 1
    • 2
  1. 1.School of Environment and ResourcesDalian Minzu UniversityDalianChina
  2. 2.School of Resources and EnvironmentNortheast Agricultural UniversityHarbinChina
  3. 3.Department of AnesthesiologyThe Third Affiliated Hospital of Sun Yat-Sen UniversityGuangzhouChina

Personalised recommendations