Bioprocess and Biosystems Engineering

, Volume 42, Issue 5, pp 883–896 | Cite as

Enhanced ethanol production from industrial lignocellulose hydrolysates by a hydrolysate-cofermenting Saccharomyces cerevisiae strain

  • Shuangcheng Huang
  • Tingting Liu
  • Bingyin Peng
  • Anli GengEmail author
Research Paper


Industrial production of lignocellulosic ethanol requires a microorganism utilizing both hexose and pentose, and tolerating inhibitors. In this study, a hydrolysate-cofermenting Saccharomyces cerevisiae strain was obtained through one step in vivo DNA assembly of pentose-metabolizing pathway genes, followed by consecutive adaptive evolution in pentose media containing acetic acid, and direct screening in biomass hydrolysate media. The strain was able to coferment glucose and xylose in synthetic media with the respective maximal specific rates of glucose and xylose consumption, and ethanol production of 3.47, 0.38 and 1.62 g/g DW/h, with an ethanol titre of 41.07 g/L and yield of 0.42 g/g. Industrial wheat straw hydrolysate fermentation resulted in maximal specific rates of glucose and xylose consumption, and ethanol production of 2.61, 0.54 and 1.38 g/g DW/h, respectively, with an ethanol titre of 54.11 g/L and yield of 0.44 g/g. These are among the best for wheat straw hydrolysate fermentation through separate hydrolysis and cofermentation.


Saccharomyces cerevisiae Lignocellulose hydrolysate Cellulosic ethanol In vivo DNA assembly Hexose and pentose co-fermentation Inhibitory chemicals 



This study was funded by the Science and Engineering Research Council of the Agency for Science Technology and Research (A*STAR) Singapore (Grant no. 092 139 0035). The authors are grateful for the industrial lignocellulose hydrolysate samples provided by Teck Guan Holdings Sdn Bhd, Tawau, Malaysia and Inbicon A/S, Fredericia, Denmark. We are also thankful for the internship opportunities provided by Ngee Ann Polytechnic Singapore to Shuangcheng Huang and Tingting Liu.

Supplementary material

449_2019_2090_MOESM1_ESM.doc (60 kb)
Supplementary material 1 (DOC 59 KB)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Shuangcheng Huang
    • 1
  • Tingting Liu
    • 1
  • Bingyin Peng
    • 1
  • Anli Geng
    • 1
    Email author
  1. 1.School of Life Sciences and Chemical TechnologyNgee Ann PolytechnicSingaporeSingapore

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