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Bioprocess and Biosystems Engineering

, Volume 42, Issue 5, pp 897–900 | Cite as

Effect of 2-deoxy-d-glucose on gellan gum biosynthesis by Sphingomonas paucimobilis

  • Guilan ZhuEmail author
  • Na Guo
  • Yanan Yong
  • Yawen Xiong
  • Qunyi Tong
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Abstract

2-Deoxy-d-glucose (2-DG) is a non-metabolizable glucose analogue and competitive inhibitor of glycolysis. Effect of 2-DG on gellan gum biosynthesis by Sphingomonas paucimobilis ATCC31461 were studied in this research. The concentration and the addition time of 2-DG significantly affected the biomass and gellan gum accumulation. The maximum gellan gum yield of 20.78 g/L was obtained with the addition of 50 µg/L of 2-DG at 24 h. The mechanism of 2-DG addition favoring to gellan production was revealed by determining the activities of key enzymes. Results indicated that 2-DG addition increased the activities of glucosyltransferase and inhibited UDP-glucose pyrophosphorylase activity. The result indicated that 2-DG inhibited glycolysis and changed metabolic driving force to activate gellan gum biosynthesis metabolism pathways.

Keywords

2-Deoxy-d-glucose Gellan gum Sphingomonas paucimobilis ATCC 31461 

Notes

Acknowledgements

We are grateful for financial support from the National Natural Science Foundation of China (31401657).

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Guilan Zhu
    • 1
    Email author
  • Na Guo
    • 1
  • Yanan Yong
    • 1
  • Yawen Xiong
    • 1
  • Qunyi Tong
    • 2
  1. 1.Department of Life ScienceHefei Normal UniversityHefeiChina
  2. 2.School of Food Science and TechnologyJiangnan UniversityWuxiChina

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