Bioprocess and Biosystems Engineering

, Volume 42, Issue 5, pp 741–752 | Cite as

Performance and microbial community profiles in pilot-scale biofilter for the simultaneous removal of ammonia, iron and manganese at different manganese concentrations

  • Qingfeng ChengEmail author
  • Yang Huang
  • Lichao Nengzi
  • Jie Zhang
Research Paper


To accelerate extensive application of biological manganese removal technology, a pilot-scale biofilter for ammonia, iron and manganese removal was constructed to investigate the removal performance and microbial community profiles at different manganese concentrations. When manganese in influent increased from 1 to 10 mg/L, the pollutants were completely removed. Ammonia and iron was slightly changed along the filter depth, while manganese obviously increased. In 0 m of the filter depth, the abundance of Gallionella (iron oxidizing bacteria, IOB) increased, while Crenothrix (IOB) decreased. The abundance of Gallionella (manganese oxidizing bacteria, MnOB) in 0.4 and 0.8 m increased to 16.82% and 12.37%, respectively; and Crenothrix (MnOB) in 0.8 m increased to 19.95%, but decreased to 25.08% in 0.4 m. The abundance of ammonia oxidizing bacteria (AOB, Nitrosococcus) decreased in 0.4 and 0.8 m. The biofilter presented a high ability to remove manganese, and had a broad application prospect.

Graphical abstract


Ammonia, iron and manganese removal Microbial community structure Biofilter Manganese concentration Real groundwater 



This work was kindly supported by National Natural Science Foundation of China (51808062), and Department of Science and Technology of Sichuan Province (19ZDYF0150).

Compliance with ethical standards

Conflict of interest

The authors have no conflict of interest to declare.

Supplementary material

449_2019_2077_MOESM1_ESM.doc (506 kb)
Supplementary material 1 (DOC 506 KB)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Qingfeng Cheng
    • 1
    Email author
  • Yang Huang
    • 1
  • Lichao Nengzi
    • 2
  • Jie Zhang
    • 3
  1. 1.College of Resources and EnvironmentChengdu University of Information TechnologyChengduPeople’s Republic of China
  2. 2.College of Resources and EnvironmentXichang UniversityXichangPeople’s Republic of China
  3. 3.State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental EngineeringHarbin Institute of TechnologyHarbinPeople’s Republic of China

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