Bioprocess and Biosystems Engineering

, Volume 42, Issue 5, pp 687–696 | Cite as

Lipid production by Cryptococcus albidus using biowastes hydrolysed by indigenous microbes

  • Ezhaveni Sathiyamoorthi
  • Prasun Kumar
  • Beom Soo KimEmail author
Research Paper


The efficiency of Cryptococcusalbidus was evaluated for its abilities to assimilate onion and apple hydrolysates as a medium for lipid production. Onion waste (OW) and apple waste (AW) were hydrolysed at an organic load of 2% total solids by indigenous microbes under mesophilic conditions. The indigenous microbes effectively hydrolysed both wastes giving the highest reducing sugar content of 4.8 g/L and 10.8 g/L with OW and AW hydrolysates, respectively. The microbiome analysis revealed that most of the indigenous microbes belonged to genus Bacillus and a significant population of α-proteobacteria and γ-proteobacteria were also present. Cell retention culture of C. albidus at a dilution rate of 0.01 h−1 resulted in a total dry cell weight (DCW) of 13.5 g/L with an intracellular lipid content of 20.0% at 168 h, corresponding to an enhancement of 3.48-folds and 2.37-folds in DCW and lipid concentration, respectively, as compared to batch fermentation.


Biodiesel Cryptococcus albidus Lignocellulose Oleaginous yeast Microbiome analysis Waste hydrolysis 



This research was supported by the National Research Foundation of Korea (NRF-2017R1A2B4002371). ES and PK are thankful for the financial support provided by a Brain Korea 21 plus project.

Supplementary material

449_2019_2073_MOESM1_ESM.docx (913 kb)
Supplementary material 1 (DOCX 912 KB)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Ezhaveni Sathiyamoorthi
    • 1
  • Prasun Kumar
    • 1
  • Beom Soo Kim
    • 1
    Email author
  1. 1.Department of Chemical EngineeringChungbuk National UniversityCheongjuRepublic of Korea

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