Bioprocess and Biosystems Engineering

, Volume 42, Issue 3, pp 499–512 | Cite as

Production of laccase by repeated batch semi-solid fermentation using wheat straw as substrate and support for fungal growth

  • Antriksh Gupta
  • Asim Kumar JanaEmail author
Research Paper


Repeated batch semi-solid fermentation (sSF) process using wheat straw substrate and fungal growth of Ganoderma lucidum on solid substrate was studied for production of laccase. pH showed significant effect on laccase production. Highest laccase activity with pH controlled to 5.0 in batch sSF was 15257.2 ± 353.4 U L− 1 on 9th day. In repeated batch process at pH 5.0, insoluble biomass substrate and fungal growth were reused after liquid part of medium was replaced with glucose, ammonium phosphate (best nitrogen source) and combined glucose and ammonium phosphate solution separately. Refilled to 80% w v− 1 of initial soluble sugar of first batch resulted in highest laccase production with peak activity after 4 days from replacement. Production of enzyme increased from 15257.2 U L− 1 in first batch to cumulative 90164.4 U L− 1 in 29 days after six repeated batches, productivity increased from 1680.2 to 3110.3 U L− 1 day− 1 (∼ 1.9 times) due to reductions in inhibitory effects and time required for fungal growth. Utilization of wheat straw in repeated batch sSF was supported by composition analysis and morphological changes (scanning electron microscopy) of substrate. Economic production of laccase using agricultural residues in repeated batch sSF could be possible.


White rot fungi Semi-solid fermentation Repeated batch process Laccase production 



Mr Antriksh Gupta gratefully acknowledges Ministry of Human Resource Development (MHRD), Govt. of India, for providing the fellowship during the study. All authors are highly thankful to National Institute of Technology (NIT), Jalandhar, for providing grants and administrative supports for the study.

Compliance with ethical standards

Conflict of interest

Authors declare that they have no conflict of interest.

Supplementary material

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Supplementary material 1 (PDF 173 KB)
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Supplementary material 2 (PDF 113 KB)
449_2018_2053_MOESM3_ESM.pdf (100 kb)
Supplementary material 3 (PDF 99 KB)
449_2018_2053_MOESM4_ESM.pdf (10 kb)
Supplementary material 4 (PDF 10 KB)


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© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of BiotechnologyDr. B. R. Ambedkar National Institute of TechnologyJalandharIndia

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