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A novel osmotic pressure strategy to improve erythritol production by Yarrowia lipolytica from glycerol

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Abstract

Polyethylene glycol (PEG) is polymer that was used to replace NaCl (reference media) as an osmotic stress agent for the synthesis of erythritol by the osmophilic yeast Yarrowia lipolytica. Two strains, the wild-type strain IMUFRJ 50682 and the lab strain W29, were grown in the presence of PEG of different molecular weights. For strain IMUFRJ 50682, the erythritol titer was increased by 40% in the presence of PEG2000 as compared to the reference media (with NaCl). A similar increase was also observed for strain W29, except that it occurred in the presence of PEG6000. Moreover, in those experimental conditions neither strain produced mannitol, in contrast to the control medium. These results highlight that PEG could be used to increase erythritol productivity and to simultaneously inhibit mannitol synthesis, representing a good substitute for NaCl as an osmotic stress agent.

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Acknowledgements

The financial support of FAPERJ Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro, Brazil, CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Ministry of Education, Brazil) and WBI (Wallonie Bruxelles International, Belgium, Grant no. 154157) is greatly acknowledged. The authors wish to thank Dr. Theron for editing the English language of the text.

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Authors and Affiliations

  1. Biochemical Engineering Department, Escola de Química, Universidade Federal do Rio de Janeiro, Av. Athos da Silveira Ramos, 149, CT, Bl. E, Rio de Janeiro, 21941-909, Brazil

    Luana Vieira da Silva, Maria Alice Z. Coelho & Priscilla F. Fonseca Amaral

  2. Microbial Processes and Interactions, TERRA Teaching and Research Centre, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium

    Patrick Fickers

Authors
  1. Luana Vieira da Silva
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  2. Maria Alice Z. Coelho
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  3. Priscilla F. Fonseca Amaral
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  4. Patrick Fickers
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Correspondence to Priscilla F. Fonseca Amaral.

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The authors declare no financial or commercial conflict of interest.

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da Silva, L.V., Coelho, M.A.Z., Amaral, P.F.F. et al. A novel osmotic pressure strategy to improve erythritol production by Yarrowia lipolytica from glycerol. Bioprocess Biosyst Eng 41, 1883–1886 (2018). https://doi.org/10.1007/s00449-018-2001-5

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  • DOI: https://doi.org/10.1007/s00449-018-2001-5

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