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Simultaneous production of single cell oil and fumaric acid by a newly isolated yeast Aureobasidium pullulans var. aubasidani DH177

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Abstract

Microbial oils can be used for biodiesel production and fumaric acid (FA) is widely used in the food and chemical industries. In this study, the production of lipids and FA by Aureobasidium pullulans var. aubasidani DH177 was investigated. A high initial carbon/nitrogen ratio in the medium promoted the accumulation of lipids and FA. When the medium contained 12.0% glucose and 0.2% NH4NO3, the yeast strain DH177 accumulated 64.7% (w/w) oil in its cells, 22.4 g/l cell biomass and 32.3 g/l FA in a 5-L batch fermentation. The maximum yields of oil and FA were 0.12 g/g and 0.27 g/g of consumed sugar, respectively. The compositions of the produced fatty acids were C14:0 (0.6%), C16:0 (24.9%), C16:1 (4.4%), C18:0 (2.1%), C18:1 (57.6%), and C18:2 (10.2%). Biodiesel obtained from the extracted oil burned well. This study provides the pioneering utilization of the yeast strain DH177 for the integrated production of oil and FA.

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Acknowledgements

This research was supported by the Research Foundation for Advanced Talents of Qingdao Agricultural University (Grant No. 6631114335) and Taishan Scholar Construction Foundation of Shandong Province (Grant No. 6631114314).

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Authors and Affiliations

  1. College of Life Science, Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural University, Qingdao, 266109, China

    Guangyuan Wang & Zhengang Miao

  2. College of Plant Health and Medicine, The Key Laboratory of Integrated Crop Pest Management of Shandong Province, Qingdao Agricultural University, Qingdao, 266109, China

    Tingting Bai & Wenxing Liang

  3. Qingdao Agricultural Science and Technology Service Center, Qingdao, 266071, China

    Weiguang Ning

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  1. Guangyuan Wang
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Correspondence to Guangyuan Wang.

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Wang, G., Bai, T., Miao, Z. et al. Simultaneous production of single cell oil and fumaric acid by a newly isolated yeast Aureobasidium pullulans var. aubasidani DH177. Bioprocess Biosyst Eng 41, 1707–1716 (2018). https://doi.org/10.1007/s00449-018-1994-0

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