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Bioprocess and Biosystems Engineering

, Volume 41, Issue 10, pp 1417–1423 | Cite as

Improved production of jiangxienone in submerged fermentation of Cordyceps jiangxiensis under nitrogen deficiency

  • Lu-Xi Jiang
  • Li-Liang Han
  • Hui-Ping Wang
  • Jun-Wei Xu
  • Jian-Hui Xiao
Research Paper
  • 97 Downloads

Abstract

Jiangxienone produced by Cordyceps jiangxiensis exhibits significant cytotoxicity and good selectivity against various human cancer cells, especially gastric cancer cells. In this work, the effect of nitrogen deficiency on the accumulation of jiangxienone and the transcription levels of jiangxienone biosynthesis genes was studied in submerged fermentation of C. jiangxiensis. Results showed that accumulation of jiangxienone was improved under nitrogen deficiency condition. A maximal jiangxienone content of 3.2 µg/g cell dry weight was reached at 5 mM glutamine, and it was about 8.9-fold higher than that obtained at 60 mM glutamine (control). The transcription levels of the biosynthetic pathway genes hmgr and sqs and the nitrogen regulatory gene areA were upregulated by 7-, 14-, and 28-fold, respectively, in culture with 5 mM glutamine compared to the control. It was hypothesized that the jiangxienone biosynthesis may involve the mevalonate pathway in C. jiangxiensis. Taken together, our study indicated that nitrogen deficiency is an efficient strategy for enhancing jiangxienone accumulation in submerged fermentation of C. jiangxiensis, which is useful for further understanding the regulation of jiangxienone biosynthesis.

Keywords

Jiangxienone Cordyceps jiangxiensis Nitrogen deficiency Biosynthetic gene 

Notes

Acknowledgements

This research was supported by the National Nature Science Foundation of China (Nos. 21566016, 81660363), and the Guizhou High-Level Innovative Talent Support Program (No. QKH-RC-20154028).

Compliance with ethical standards

Conflict of interest

We declare that we have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Division of Applied Mycology and Biochemical PharmacyInstitute of Medicinal Biotechnology, Affiliated Hospital of Zunyi Medical UniversityZunyiPeople’s Republic of China
  2. 2.Faculty of Life Science and TechnologyKunming University of Science and TechnologyKunmingPeople’s Republic of China
  3. 3.Department of Neurology, Kunming Children’s HospitalKunming Medical UniversityKunmingPeople’s Republic of China

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