Bioprocess and Biosystems Engineering

, Volume 41, Issue 7, pp 1061–1071 | Cite as

Combined production of fucoxanthin and EPA from two diatom strains Phaeodactylum tricornutum and Cylindrotheca fusiformis cultures

  • Hui Wang
  • Yan Zhang
  • Lin Chen
  • Wentao Cheng
  • Tianzhong Liu
Research Paper


Fucoxanthin and eicosapentaenoic acid (EPA) provide significant health benefits for human population. Diatom is a potential natural livestock for the combined production of EPA and fucoxanthin. In this study, first, the effects of three important parameters including light intensity, nitrogen concentration and salinity were evaluated for the production of EPA and fucoxanthin in two diatom strains Phaeodactylum tricornutum and Cylindrotheca fusiformis. And then, two steps method based on light intensity were applied to produce EPA and fucoxanthin in large scale. Higher light intensity was first adopted for the high growth rate and lipid content of diatom, and after a period of time, light intensity was lowered to enhance the accumulation of fucoxanthin and EPA. In final, the highest EPA yields were 62.55 and 27.32 mg L−1 for P. tricornutum and C. fusiformis, and the fucoxanthin yield reached 8.32 and 6.05 mg L−1, respectively.


Fucoxanthin Eicosapentaenoic acid Diatom P. tricornutum C. fusiformis 



This work was supported by Shandong Provincial Natural Science Foundation, China (ZR2017QC007) and Marine economic innovation and development regional demonstration project of Qingdao.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Hui Wang
    • 1
    • 2
  • Yan Zhang
    • 1
    • 3
  • Lin Chen
    • 1
    • 2
  • Wentao Cheng
    • 1
    • 2
  • Tianzhong Liu
    • 1
    • 2
  1. 1.CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdaoPeople’s Republic of China
  2. 2.Shandong Provincial Key Laboratory of Bioenergy Resources, Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdaoPeople’s Republic of China
  3. 3.University of Chinese Academy of SciencesBeijingPeople’s Republic of China

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