Bioprocess and Biosystems Engineering

, Volume 41, Issue 6, pp 803–809 | Cite as

Growth medium sterilization using decomposition of peracetic acid for more cost-efficient production of omega-3 fatty acids by Aurantiochytrium

  • Chang-Ho Cho
  • Won-Sub Shin
  • Do-Wook Woo
  • Jong-Hee Kwon
Research Paper


Aurantiochytrium can produce significant amounts of omega-3 fatty acids, specifically docosahexaenoic acid and docosapentaenoic acid. Use of a glucose-based medium for heterotrophic growth is needed to achieve a high growth rate and production of abundant lipids. However, heat sterilization for reliable cultivation is not appropriate to heat-sensitive materials and causes a conversion of glucose via browning (Maillard) reactions. Thus, the present study investigated the use of a direct degradation of Peracetic acid (PAA) for omega-3 production by Aurantiochytrium. Polymer-based bioreactor and glucose-containing media were chemically co-sterilized by 0.04% PAA and neutralized through a reaction with ferric ion (III) in HEPES buffer. Mono-cultivation was achieved without the need for washing steps and filtration, thereby avoiding the heat-induced degradation and dehydration of glucose. Use of chemically sterilized and neutralized medium, rather than heat-sterilized medium, led to a twofold faster growth rate and greater productivity of omega-3 fatty acids.


Aurantiochytrium Chemical sterilization Peracetic acid Omega-3 Maillard reaction 



This research was supported by the Basic Science Research Program, through the National Research Foundation of Korea (NRF), and was funded by the Ministry of Science, ICT & Future Planning (2015R1C1A1A01054303). This work was supported by the Fund for New Professor research foundation Program (2015-04-012), Gyeongsang National University, 2015.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Chang-Ho Cho
    • 1
    • 3
  • Won-Sub Shin
    • 2
  • Do-Wook Woo
    • 1
    • 3
  • Jong-Hee Kwon
    • 1
    • 3
  1. 1.Department of Food Science and Technology and Institute of Agriculture and Life ScienceGyeongsang National UniversityJinjuRepublic of Korea
  2. 2.Department of Chemical and Biomolecular EngineeringKAISTDaejeonRepublic of Korea
  3. 3.Division of Applied Life Sciences (BK21 plus)Gyeongsang National UniversityJinjuRepublic of Korea

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