Bioprocess and Biosystems Engineering

, Volume 41, Issue 6, pp 781–791 | Cite as

Influence of Zn(II) stress-induction on component variation and sorption performance of extracellular polymeric substances (EPS) from Bacillus vallismortis

  • Peifei Ding
  • Weifeng Song
  • Ziheng Yang
  • Jingyi Jian
Research Paper


Bacillus vallismortis (B. vallismortis), an aerobic heterotrophic bacteria, was screened in a laboratory pilot study, to assess the interaction between the heavy metal Zn(II) and extracellular polymeric substances (EPS). The influence of Zn(II) stress on EPS production, component variation, and sorption performance, was investigated. The characteristics of B. vallismortis EPS formed under stress were analyzed using FTIR, 3D-EEM and XPS. EPS was used as an adsorbent and the adsorption capacity and adsorption behavior of EPS formed with and without Zn(II) stress, were compared and assessed. Results showed that the production of polysaccharides and proteins, the main components of EPS, were promoted under Zn(II) stress. The types of EPS functional groups observed remained the same with and without heavy metal stress, but their concentrations were increased. Due to stress-induction, the adsorption capacity of Zn-EPS was significantly enhanced compared with the control-EPS. Specific EPS produced by B. vallismortis in the presence of Zn(II) stress, could have a wide range of potential applications, allowing optimization and improvement of the capacity of EPS to remove heavy metals from effluent.


Stress/induction Heavy metals Extracellular polymeric substances Characteristic Adsorption 



This work was supported by the Guangdong Provincial Science and Technology Planning Project (No.2014A020209077).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. 1.
    Nourbakhsh M, Sag̃ Y, Özer D, Aksu Z, Kutsal T, Çag̃Lar A (1994) A comparative study of various biosorbents for removal of chromium(VI) ions from industrial waste waters. Process Biochem 29:1–5CrossRefGoogle Scholar
  2. 2.
    More TT, Yadav JS, Yan S, Tyagi RD, Surampalli RY (2014) Extracellular polymeric substances of bacteria and their potential environmental applications. J Environ Manag 144:1–25CrossRefGoogle Scholar
  3. 3.
    Sheng GP, Yu HQ, Li XY (2010) Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems: a review. Biotechnol Adv 28:882CrossRefPubMedGoogle Scholar
  4. 4.
    Deschatre M, Ghillebaert F, Guezennec J, Colin CS (2013) Sorption of copper(II) and silver(I) by four bacterial exopolysaccharides. Appl Biochem Biotechnol 171:1313–1327CrossRefPubMedGoogle Scholar
  5. 5.
    Hou W, Ma Z, Sun L, Han M, Lu J, Li Z, Mohamad OA, Wei G (2013) Extracellular polymeric substances from copper-tolerance Sinorhizobium meliloti immobilize Cu2+. J Hazard Mater 261C:614–620CrossRefGoogle Scholar
  6. 6.
    Yue ZB, Li Q, Li CC, Chen TH, Wang J (2015) Component analysis and heavy metal adsorption ability of extracellular polymeric substances (EPS) from sulfate reducing bacteria. Bioresour Technol 194:399–402CrossRefPubMedGoogle Scholar
  7. 7.
    Chen B, Li F, Liu N, Ge F, Xiao H, Yang Y (2015) Role of extracellular polymeric substances from Chlorella vulgaris in the removal of ammonium and orthophosphate under the stress of cadmium. Bioresour Technol 190:299–306CrossRefPubMedGoogle Scholar
  8. 8.
    Cheng YJ, SONG WF, LIN LT, DONG M (2016) Anaerobic biodegradation and mechanism of aniline aerofloat. China Environ Sci 4:033–038Google Scholar
  9. 9.
    Raunkjær K, Hvitved-Jacobsen T, Nielsen PH (1994) Measurement of pools of protein, carbohydrate and lipid in domestic wastewater. Water Res 28:251–262CrossRefGoogle Scholar
  10. 10.
    Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem 72:248–254CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Sun Y, Clinkenbeard KD, Clarke C, Cudd L, Highlander SK, Dabo SM (1999) Pasteurella haemolytica leukotoxin induced apoptosis of bovine lymphocytes involves DNA fragmentation. Vet Microbiol 65:153–166CrossRefPubMedGoogle Scholar
  12. 12.
    Raliya R, Tarafdar JC, Mahawar H, Kumar R, Gupta P, Mathur T, Kaul RK, PraveenKumar, Kalia A, Gautam R (2014) ZnO nanoparticles induced exopolysaccharide production by B. subtilis strain JCT1 for arid soil applications. Int J Biol Macromol 65:362CrossRefPubMedGoogle Scholar
  13. 13.
    Ueshima M, Ginn BR, Haack EA, Szymanowski JES, Fein JB (2008) Cd adsorption onto Pseudomonas putida in the presence and absence of extracellular polymeric substances. Geochim Cosmochim Acta 72:5885–5895CrossRefGoogle Scholar
  14. 14.
    Grąz M, Pawlikowska-Pawlęga B, Jarosz-Wilkołazka A (2011) Growth inhibition and intracellular distribution of Pb ions by the white-rot fungus Abortiporus biennis. Int Biodeter Biodegrad 65:124–129CrossRefGoogle Scholar
  15. 15.
    Chen A, Shang C, Zeng G, Chen G, Shao J, Zhang J, Huang H (2015) Extracellular secretions of Phanerochaete chrysosporium on Cd toxicity. Int Biodeter Biodegrad 105:73–79CrossRefGoogle Scholar
  16. 16.
    Chen A, Zeng G, Chen G, Liu L, Shang C, Hu X, Lu L, Chen M, Zhou Y, Zhang Q (2014) Plasma membrane behavior, oxidative damage, and defense mechanism in Phanerochaete chrysosporium under cadmium stress. Process Biochem 49:589–598CrossRefGoogle Scholar
  17. 17.
    Kim SH, Kim SK, Jung KH, Kim YK, Hwang HC, Ryu SG, Chai YG (2013) Proteomic analysis of the oxidative stress response induced by low-dose hydrogen peroxide in Bacillus anthracis. J Microbiol Biotechn 23:750–758CrossRefGoogle Scholar
  18. 18.
    Wan J, Zeng G, Huang D, Huang C, Lai C, Li N, Wei Z, Xu P, He X, Lai M (2015) The oxidative stress of phanerochaete chrysosporium against lead toxicity. Appl Biochem Biotech 175:1981–1991CrossRefGoogle Scholar
  19. 19.
    Naik MM, Pandey A, Dubey SK (2012) Biological characterization of lead-enhanced exopolysaccharide produced by a lead resistant Enterobacter cloacae strain P2B. Biodegradation 23:775–783CrossRefPubMedGoogle Scholar
  20. 20.
    Coble PG (1996) Characterization of marine and terrestrial DOM in seawater using excitation-emission matrix spectroscopy. Mar Chem 51:325–346CrossRefGoogle Scholar
  21. 21.
    Chen W, Westerhoff P, Leenheer JA, Booksh K (2003) Fluorescence excitation-emission matrix regional integration to quantify spectra for dissolved organic matter. Environ Sci Technol 37:5701–5710CrossRefPubMedGoogle Scholar
  22. 22.
    Stedmon CA, Markager S, Bro R (2003) Tracing dissolved organic matter in aquatic environments using a new approach to fluorescence spectroscopy. Mar Chem 82:239–254CrossRefGoogle Scholar
  23. 23.
    Guo XJ, He LS, Li Q, Yuan DH, Deng Y (2014) Investigating the spatial variability of dissolved organic matter quantity and composition in Lake Wuliangsuhai. Ecol Eng 62:93–101CrossRefGoogle Scholar
  24. 24.
    Wu F, Tanoue E (2001) Isolation and partial characterization of dissolved copper-complexing ligands in streamwaters. Environ Sci Technol 35:3646CrossRefPubMedGoogle Scholar
  25. 25.
    Wang J, Li Q, Li MM, Chen TH, Zhou YF, Yue ZB (2014) Competitive adsorption of heavy metal by extracellular polymeric substances (EPS) extracted from sulfate reducing bacteria. Bioresour Technol 163:374–376CrossRefPubMedGoogle Scholar
  26. 26.
    Wang Z, Gao M, Wang Z, She Z, Chang Q, Sun C, Zhang J, Ren Y, Yang N (2013) Effect of salinity on extracellular polymeric substances of activated sludge from an anoxic–aerobic sequencing batch reactor. Chemosphere 93:2789–2795CrossRefPubMedGoogle Scholar
  27. 27.
    Wang ZW, Wu ZC, Tang SJ (2009) Characterization of dissolved organic matter in a submerged membrane bioreactor by using three-dimensional excitation and emission matrix fluorescence spectroscopy. Water Res 43:1533–1540CrossRefPubMedGoogle Scholar
  28. 28.
    Yin Y, Hu Y, Xiong F (2011) Sorption of Cu(II) and Cd(II) by extracellular polymeric substances (EPS) from Aspergillus fumigatus. Int Biodeter Biodegrad 65:1012–1018CrossRefGoogle Scholar
  29. 29.
    Fang L, Wei X, Cai P, Huang Q, Chen H, Liang W, Rong X (2011) Role of extracellular polymeric substances in Cu(II) adsorption on Bacillus subtilis and Pseudomonas putida. Biores Technol 102:1137–1141CrossRefGoogle Scholar
  30. 30.
    Jiang W, Saxena A, Song B, Ward BB, Beveridge TJ, Myneni SC (2004) Elucidation of functional groups on gram-positive and gram-negative bacterial surfaces using infrared spectroscopy. Langmuir ACS J Surf Colloids 20:11433–11442CrossRefGoogle Scholar
  31. 31.
    Xu Y, Hou M, Ruan J, Qu M, Sun H, Xu J, Zhou S (2014) Effect of magnetic field on surface properties of CrA and its extracellular polymeric substances (EPS). J Adhes Sci Technol 28:2196–2208CrossRefGoogle Scholar
  32. 32.
    Badireddy AR, Chellam S, Gassman PL, Engelhard MH, Lea AS, Rosso KM (2010) Role of extracellular polymeric substances in bioflocculation of activated sludge microorganisms under glucose-controlled conditions. Water Res 44:4505–4516CrossRefPubMedGoogle Scholar
  33. 33.
    Lin D, Ma W, Jin Z, Wang Y, Huang Q, Cai P (2015) Interactions of EPS with soil minerals: a combination study by ITC and CLSM. Colloids Surf B Biointerfaces 138:10–16CrossRefPubMedGoogle Scholar
  34. 34.
    Rouxhet PG, Mozes N, Dengis PB, Dufrêne YF, Gerin PA, Genet MJ (1994) Application of X-ray photoelectron spectroscopy to microorganisms. Colloids Surf B Biointerfaces 2:347–369CrossRefGoogle Scholar
  35. 35.
    Yang Y, Wikieł AJ, Dall’Agnol LT, Eloy P, Genet MJ, Moura JJ, Sand W, Dupont-Gillain CC, Rouxhet PG (2016) Proteins dominate in the surface layers formed on materials exposed to extracellular polymeric substances from bacterial cultures. Biofouling 32:95–108CrossRefPubMedGoogle Scholar
  36. 36.
    Li Y, Li Q, Fengying Y, Bao J, Hu Z, Zhu W, Zhao Y, Lin Z, Dong Q (2015) Chromium (VI) detoxification by oxidation and flocculation of exopolysaccharides from Arthrobacter sp. B4. Int J Biol Macromol 81:235–240CrossRefPubMedGoogle Scholar
  37. 37.
    Bautista BET, Wikieł AJ, Datsenko I, Vera M, Sand W, Seyeux A, Zanna S, Frateur I, Marcus P (2014) Influence of extracellular polymeric substances (EPS) from Pseudomonas NCIMB 2021 on the corrosion behaviour of 70Cu–30Ni alloy in seawater. J Electroanal Chem 737:184–197CrossRefGoogle Scholar
  38. 38.
    Szcześ A, Czemierska M, Jarosz-Wilkołazka A (2016) Calcium carbonate formation on mica supported extracellular polymeric substance produced by Rhodococcus opacus. J Solid State Chem 242:212–221CrossRefGoogle Scholar
  39. 39.
    Sharma M, Kaushik A, Somvir, Bala K, Kamra A (2008) Sequestration of chromium by exopolysaccharides of Nostoc and Gloeocapsa from dilute aqueous solutions. J Hazard Mater 157:315–318CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Peifei Ding
    • 1
  • Weifeng Song
    • 1
  • Ziheng Yang
    • 1
  • Jingyi Jian
    • 2
  1. 1.College of Environmental Science and EngineeringGuangdong University of TechnologyGuangzhouPeople’s Republic of China
  2. 2.Environmental Technology Center of Panyu DistrictGuangzhouPeople’s Republic of China

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