Bioprocess and Biosystems Engineering

, Volume 41, Issue 4, pp 519–530 | Cite as

Cultivation of newly isolated microalgae Coelastrum sp. in wastewater for simultaneous CO2 fixation, lipid production and wastewater treatment

  • Shokouh Mousavi
  • Ghasem D. Najafpour
  • Maedeh Mohammadi
  • Mohammad Hasan Seifi
Research Paper


Cultivation of microalgae in wastewater is a promising and cost-effective approach for both CO2 biofixation and wastewater remediation. In this study, a new strain of Coelastrum sp. was isolated from cattle manure leachate. The isolated microalgae were then cultivated in wastewater. Effects of different sCOD concentrations (600, 750, 900, 1050 mg L−1) and light intensities (1000, 2300, 4600, 6900 and 10000 Lux) on biomass production, CO2 consumption rate and nutrient removal from wastewater were investigated. The results showed that maximum cell growth and CO2 consumption rate were 2.71 g L−1 and 53.12 mg L−1 day−1, respectively, which were obtained in the wastewater with 750 mg L−1 sCOD and under the light intensity of 6900 Lux. The microalgae were able to completely consume all CO2 after incubation period of 4 days. The highest sCOD, total Kjeldahl nitrogen (TKN), nitrate and total phosphorous (TP) removal at such conditions were 53.45, 91.18, 87.51 and 100%, respectively. The lipid content of microalgal biomass was also measured under different light intensities; maximum amount of lipid was determined to be 50.77% under illumination of 2300 Lux. Finally, the CO2 consumption rate and biomass productivity of microalgae in semi-batch culture with continuous gas flow (CO2 6%:N2 94%) were investigated. The rate of CO2 consumption and biomass productivity were 0.528 and 0.281 g L−1 day−1, respectively. The TKN, nitrate, TP and sCOD removal rate of microalgae were 83.51, 80.91, 100, 41.4%, respectively.


Microalgae Coelastrum sp. CO2 fixation Wastewater treatment 



The authors gratefully acknowledge Biotechnology Research Lab., Babol Noshirvani University of Technology for the facilities provided to conduct present research. Also, special thanks are extended to Mazandaran Gas Company, Sari, Iran, for the financial support for the present research through research Grant # 11226.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. 1.
    da Rosa GM, Moraes L, Cardias BB, Costa JAV (2015) Chemical absorption and CO2 biofixation via the cultivation of Spirulina in semicontinuous mode with nutrient recycle. Bioresour Technol 192:321–327CrossRefGoogle Scholar
  2. 2.
    Beerling DJ, Royer DL (2011) Convergent cenozoic CO2 history. Nat Geosci 4(7):418–420CrossRefGoogle Scholar
  3. 3.
    Raeesossadati M, Ahmadzadeh H, McHenry M, Moheimani N (2014) CO2 bioremediation by microalgae in photobioreactors: impacts of biomass and CO2 concentrations, light, and temperature. Algal Res 6:78–85CrossRefGoogle Scholar
  4. 4.
    Jin H-F, Lim B-R, Lee K (2006) Influence of nitrate feeding on carbon dioxide fixation by microalgae. J Environ Sci Health A 41(12):2813–2824CrossRefGoogle Scholar
  5. 5.
    Wang B, Li Y, Wu N, Lan CQ (2008) CO2 bio-mitigation using microalgae. Appl Microbiol Biotechnol 79(5):707–718CrossRefGoogle Scholar
  6. 6.
    Mennaa FZ, Arbib Z, Perales JA (2015) Urban wastewater treatment by seven species of microalgae and an algal bloom: biomass production, N and P removal kinetics and harvestability. Water Res 83:42–51CrossRefGoogle Scholar
  7. 7.
    Ouyang Y, Zhao Y, Sun S, Hu C, Ping L (2015) Effect of light intensity on the capability of different microalgae species for simultaneous biogas upgrading and biogas slurry nutrient reduction. Int Biodeterior Biodegrad 104:157–163CrossRefGoogle Scholar
  8. 8.
    Maity JP, Bundschuh J, Chen C-Y, Bhattacharya P (2014) Microalgae for third generation biofuel production, mitigation of greenhouse gas emissions and wastewater treatment: present and future perspectives—a mini review. Energy 78:104–113CrossRefGoogle Scholar
  9. 9.
    Ruiz-Martinez A, Garcia NM, Romero I, Seco A, Ferrer J (2012) Microalgae cultivation in wastewater: nutrient removal from anaerobic membrane bioreactor effluent. Bioresour Technol 126:247–253CrossRefGoogle Scholar
  10. 10.
    Wang M, Kuo-Dahab WC, Dolan S, Park C (2014) Kinetics of nutrient removal and expression of extracellular polymeric substances of the microalgae, Chlorella sp. and Micractinium sp., in wastewater treatment. Bioresour Technol 154:131–137CrossRefGoogle Scholar
  11. 11.
    Moussa ID-B, Chtourou H, Karray F, Sayadi S, Dhouib A (2017) Nitrogen or phosphorus repletion strategies for enhancing lipid or carotenoid production from Tetraselmis marina. Bioresour Technol 238:325–332CrossRefGoogle Scholar
  12. 12.
    Chen C-Y, Chang Y-H, Chang H-Y (2016) Outdoor cultivation of Chlorella vulgaris FSP-E in vertical tubular-type photobioreactors for microalgal protein production. Algal Res 13:264–270CrossRefGoogle Scholar
  13. 13.
    Eldalatony MM, Kabra AN, Hwang J-H, Govindwar SP, Kim K-H, Kim H, Jeon B-H (2016) Pretreatment of microalgal biomass for enhanced recovery/extraction of reducing sugars and proteins. Bioprocess Biosyst Eng 39(1):95–103CrossRefGoogle Scholar
  14. 14.
    Zeng X, Danquah MK, Zhang S, Zhang X, Wu M, Chen XD, Ng I-S, Jing K, Lu Y (2012) Autotrophic cultivation of Spirulina platensis for CO2 fixation and phycocyanin production. Chem Eng J 183:192–197CrossRefGoogle Scholar
  15. 15.
    Wuang SC, Khin MC, Chua PQD, Luo YD (2016) Use of Spirulina biomass produced from treatment of aquaculture wastewater as agricultural fertilizers. Algal Res 15:59–64CrossRefGoogle Scholar
  16. 16.
    Nayak M, Karemore A, Sen R (2016) Performance evaluation of microalgae for concomitant wastewater bioremediation, CO2 biofixation and lipid biosynthesis for biodiesel application. Algal Res 16:216–223CrossRefGoogle Scholar
  17. 17.
    Soydemir G, Keris-Sen UD, Sen U, Gurol MD (2016) Biodiesel production potential of mixed microalgal culture grown in domestic wastewater. Bioprocess Biosyst Eng 39(1):45–51CrossRefGoogle Scholar
  18. 18.
    Guldhe A, Ansari FA, Singh P, Bux F (2017) Heterotrophic cultivation of microalgae using aquaculture wastewater: a biorefinery concept for biomass production and nutrient remediation. Ecol Eng 99:47–53CrossRefGoogle Scholar
  19. 19.
    Choi HJ, Lee SM (2015) Effect of the N/P ratio on biomass productivity and nutrient removal from municipal wastewater. Bioprocess Biosyst Eng 38(4):761–766CrossRefGoogle Scholar
  20. 20.
    Heo S-W, Ryu B-G, Nam K, Kim W, Yang J-W (2015) Simultaneous treatment of food-waste recycling wastewater and cultivation of Tetraselmis suecica for biodiesel production. Bioprocess Biosyst Eng 38(7):1393–1398CrossRefGoogle Scholar
  21. 21.
    Moraes L, da Rosa GM, Cardias BB, dos Santos LO, Costa JAV (2016) Microalgal biotechnology for greenhouse gas control: carbon dioxide fixation by Spirulina sp. at different diffusers. Ecol Eng 91:426–431CrossRefGoogle Scholar
  22. 22.
    Tebbani S, Lopes F, Filali R, Dumur D, Pareau D (2014) Nonlinear predictive control for maximization of CO2 bio-fixation by microalgae in a photobioreactor. Bioprocess Biosyst Eng 37(1):83–97CrossRefGoogle Scholar
  23. 23.
    Razzak SA, Ali SAM, Hossain MM, Mouanda AN (2016) Biological CO2 fixation using Chlorella vulgaris and its thermal characteristics through thermogravimetric analysis. Bioprocess Biosyst Eng 39(11):1651–1658CrossRefGoogle Scholar
  24. 24.
    Gonçalves A, Simões M, Pires J (2014) The effect of light supply on microalgal growth, CO2 uptake and nutrient removal from wastewater. Energy Convers Manage 85:530–536CrossRefGoogle Scholar
  25. 25.
    Shen Q-H, Jiang J-W, Chen L-P, Cheng L-H, Xu X-H, Chen H-L (2015) Effect of carbon source on biomass growth and nutrients removal of Scenedesmus obliquus for wastewater advanced treatment and lipid production. Bioresour Technol 190:257–263CrossRefGoogle Scholar
  26. 26.
    Basu S, Roy AS, Mohanty K, Ghoshal AK (2013) Enhanced CO2 sequestration by a novel microalga: Scenedesmus obliquus SA1 isolated from bio-diversity hotspot region of Assam, India. Bioresour Technol 143:369–377CrossRefGoogle Scholar
  27. 27.
    Saba F, Papizadeh M, Khansha J, Sedghi M, Rasooli M, Amoozegar MA, Soudi MR, Fazeli SAS (2017) A rapid and reproducible genomic DNA extraction protocol for sequence-based identification of archaea, bacteria, cyanobacteria, diatoms, fungi, and green algae. J Med Bacteriol 5(3–4):22–28Google Scholar
  28. 28.
    Olmos J, Paniagua J, Contreras R (2000) Molecular identification of Dunaliella sp. utilizing the 18S rDNA gene. Lett Appl Microbiol 30(1):80–84CrossRefGoogle Scholar
  29. 29.
    Tamura K, Nei M (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10(3):512–526Google Scholar
  30. 30.
    Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33(7):1870–1874CrossRefGoogle Scholar
  31. 31.
    Arbib Z, Ruiz J, Álvarez-Díaz P, Garrido-Perez C, Perales JA (2014) Capability of different microalgae species for phytoremediation processes: wastewater tertiary treatment, CO2 bio-fixation and low cost biofuels production. Water Res 49:465–474CrossRefGoogle Scholar
  32. 32.
    APHA, AWWA, WEF (2012) Standard methods for the examination of water and wastewater, 22nd edn. American Public Health Association, Washington, DCGoogle Scholar
  33. 33.
    Ge S, Champagne P (2016) Nutrient removal, microalgal biomass growth, harvesting and lipid yield in response to centrate wastewater loadings. Water Res 88:604–612CrossRefGoogle Scholar
  34. 34.
    Ma X, Zhou W, Fu Z, Cheng Y, Min M, Liu Y, Zhang Y, Chen P, Ruan R (2014) Effect of wastewater-borne bacteria on algal growth and nutrients removal in wastewater-based algae cultivation system. Bioresour Technol 167:8–13CrossRefGoogle Scholar
  35. 35.
    Kao C-Y, Chen T-Y, Chang Y-B, Chiu T-W, Lin H-Y, Chen C-D, Chang J-S, Lin C-S (2014) Utilization of carbon dioxide in industrial flue gases for the cultivation of microalga Chlorella sp.. Bioresour Technol 166:485–493CrossRefGoogle Scholar
  36. 36.
    Hulatt CJ, Thomas DN (2011) Productivity, carbon dioxide uptake and net energy return of microalgal bubble column photobioreactors. Bioresour Technol 102(10):5775–5787CrossRefGoogle Scholar
  37. 37.
    Larsdotter K (2006) Wastewater treatment with microalgae—a literature review. Vatten 62(1):31Google Scholar
  38. 38.
    Li Y, Zhou W, Hu B, Min M, Chen P, Ruan RR (2012) Effect of light intensity on algal biomass accumulation and biodiesel production for mixotrophic strains Chlorella kessleri and Chlorella protothecoides cultivated in highly concentrated municipal wastewater. Biotechnol Bioeng 109(9):2222–2229CrossRefGoogle Scholar
  39. 39.
    Abdel-Raouf N, Al-Homaidan A, Ibraheem I (2012) Microalgae and wastewater treatment. Saudi J Biol Sci19(3):257–275CrossRefGoogle Scholar
  40. 40.
    Guedes AC, Meireles LA, Amaro HM, Malcata FX (2010) Changes in lipid class and fatty acid composition of cultures of Pavlova lutheri, in response to light intensity. J Am Oil Chem Soc 87(7):791–801CrossRefGoogle Scholar
  41. 41.
    Cheirsilp B, Torpee S (2012) Enhanced growth and lipid production of microalgae under mixotrophic culture condition: effect of light intensity, glucose concentration and fed-batch cultivation. Bioresour Technol 110:510–516CrossRefGoogle Scholar
  42. 42.
    Rosenberg JN, Oyler GA, Wilkinson L, Betenbaugh MJ (2008) A green light for engineered algae: redirecting metabolism to fuel a biotechnology revolution. Curr Opin Biotechnol 19(5):430–436CrossRefGoogle Scholar
  43. 43.
    Tang D, Han W, Li P, Miao X, Zhong J (2011) CO2 biofixation and fatty acid composition of Scenedesmus obliquus and Chlorella pyrenoidosa in response to different CO2 levels. Bioresour Technol 102(3):3071–3076CrossRefGoogle Scholar
  44. 44.
    Zhao B, Su Y, Zhang Y, Cui G (2015) Carbon dioxide fixation and biomass production from combustion flue gas using energy microalgae. Energy 89:347–357CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Shokouh Mousavi
    • 1
  • Ghasem D. Najafpour
    • 1
  • Maedeh Mohammadi
    • 1
  • Mohammad Hasan Seifi
    • 2
  1. 1.Faculty of Chemical EngineeringBabol Noshirvani University of TechnologyBabolIran
  2. 2.Mazandaran Gas CompanySariIran

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