Bioprocess and Biosystems Engineering

, Volume 41, Issue 4, pp 467–477 | Cite as

Organic acid pretreatment of oil palm trunk: effect on enzymatic saccharification and ethanol production

  • Kittipong Rattanaporn
  • Prapakorn Tantayotai
  • Theerawut Phusantisampan
  • Peerapong Pornwongthong
  • Malinee Sriariyanun
Research Paper


Effective lignocellulosic biomass saccharification is one of the crucial requirements of biofuel production via fermentation process. Organic acid pretreatments have been gained much interests as one of the high potential methods for promoting enzymatic saccharification of lignocellulosic materials due to their lower hazardous properties and lower production of inhibitory by-products of fermentation than typical chemical pretreatment methods. In this study, three organic acids, including acetic acid, oxalic acid, and citric acid, were examined for improvement of enzymatic saccharification and bioethanol production from oil palm trunk biomass. Based on response surface methodology, oxalic acid pretreated biomass released the maximum reducing sugar of 144 mg/g-pretreated biomass at the optimum condition, which was higher than untreated samples for 2.30 times. The released sugar yield of oil palm trunk also corresponded to the results of FT-IR analysis, which revealed the physical modification of cellulose and hemicellulose surface structures of pretreated biomass. Nevertheless, citric acid pretreatment is the most efficient pretreatment method to improve bioethanol fermentation of Saccharomyces cerevisiae TISTR 5606 at 1.94 times higher than untreated biomass. These results highlighted the selection of organic acid pretreatment as a potential method for biofuel production from oil palm trunk feedstocks.


Organic acid pretreatment Lignocellulosic biomass Enzymatic saccharification Bioethanol Oil palm trunk 



The authors would like to thank King Mongkut’s University of Technology, North Bangkok (Research University Grant No. KMUTNB-NRU59-08, KMUTNB-60-ART-013) for financial support of this work.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  • Kittipong Rattanaporn
    • 1
  • Prapakorn Tantayotai
    • 2
  • Theerawut Phusantisampan
    • 3
  • Peerapong Pornwongthong
    • 4
  • Malinee Sriariyanun
    • 5
  1. 1.Department of Biotechnology, Faculty of Agro-IndustryKasetsart UniversityBangkokThailand
  2. 2.Department of Microbiology, Faculty of ScienceSrinakharinwirot UniversityBangkokThailand
  3. 3.Department of Biotechnology, Faculty of Applied ScienceKMUTNBBangkokThailand
  4. 4.Department of Agro-Industrial, Food and Environmental Technology, Faculty of Applied ScienceKMUTNBBangkokThailand
  5. 5.The Sirindhorn International Thai-German Graduate School of Engineering (TGGS)KMUTNBBangkokThailand

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