Advertisement

Bioprocess and Biosystems Engineering

, Volume 41, Issue 4, pp 457–465 | Cite as

Effects of light-emitting diode (LED) with a mixture of wavelengths on the growth and lipid content of microalgae

  • Chae Hun Ra
  • Phunlap Sirisuk
  • Jang-Hyun Jung
  • Gwi-Taek Jeong
  • Sung-Koo Kim
Research Paper

Abstract

Integrations of two-phase culture for cell growth and lipid accumulation using mixed LED and green LED wavelengths were evaluated with the microalgae, Phaeodactylum tricornutum, Isochrysis galbana, Nannochloropsis salina, and Nannochloropsis oceanica. Among the single and mixed LED wavelengths, mixed LED produced higher biomass of the four microalgae, reaching 1.03 g DCW/L I. galbana, followed by 0.95 g DCW/L P. tricornutum, 0.85 g DCW/L N. salina, and 0.62 g DCW/L N. oceanica than single LED or fluorescent lights at day 10. Binary combination of blue and red LEDs could produce the high biomass and photosynthetic pigments in the four microalgae. The highest lipid accumulation during second phase with the exposure to green LED wavelengths was 56.0% for P. tricornutum, 55.2% for I. galbana, 53.0% for N. salina, and 51.0% for N. oceanica. The major fatty acid in the four microalgae was palmitic acid (C16:0) accounting for 38.3–47.3% (w/w) of the total fatty acid content.

Keywords

Microalgae Fatty acid Mixed LED Green LED Two-phase culture 

Notes

Acknowledgements

This research was a part of the project titled ‘Innovative marine production technology driven by LED-ICT convergence photo-biology (D11514915H480000110)’, funded by the Ministry of Oceans and Fisheries, Korea.

References

  1. 1.
    Roy SS, Pal R (2015) Microalgae in aquaculture: a review with special references to nutritional value and fish dietetics. Proc Zool Soc 68:1–8CrossRefGoogle Scholar
  2. 2.
    Borowitzka MA. Microbiology of fermented foods. 2, Wood BJB (1998) Algae as food Blackie Academic and Professional, London 585–602CrossRefGoogle Scholar
  3. 3.
    Kumar K, Dasgupta CN, Nayak B, Lindblad P, Das D (2011) Development of suitable photobioreactors for CO2 sequestration addressing global warming using green algae and cyanobacteria. Bioresour Technol 102:4945–4953CrossRefGoogle Scholar
  4. 4.
    Song MM, Pei HY, Hu WR, Ma GX (2013) Evaluation of the potential of 10 microalgal strains for biodiesel production. Bioresour Technol 141:245–251CrossRefGoogle Scholar
  5. 5.
    Das P, Lei W, Aziz SS, Obbard JP (2011) Enhanced algae growth in both phototrophic and mixotrophic culture under blue light. Bioresour Technol 102:3883–3887CrossRefGoogle Scholar
  6. 6.
    Ra CH, Kang CH, Jung JH, Jeong GT, Kim SK (2016) Effects of light-emitting diodes (LEDs) on the accumulation of lipid content using a two-phase culture process with three microalgae. Bioresour Technol 212:254–261CrossRefGoogle Scholar
  7. 7.
    Richmond A (2003) Handbook of microalgal culture: biotechnology and applied phycology. In: Masojídek J, Koblížek M, Torzillo G (eds) Photosynthesis in microalgae. Blackwell, Hoboken 20–39Google Scholar
  8. 8.
    Ugwu CU, Aoyagi H, Uchiyama H (2008) Photobioreactors for mass cultivation of algae. Bioresour Technol 110:4021–4028CrossRefGoogle Scholar
  9. 9.
    You T, Barnett SM (2004) Effect of light quality on production of extracellular polysacchariedes and growth rate of Porphyridium cruentum. Biochem Eng J 19:251–258CrossRefGoogle Scholar
  10. 10.
    Kim TH, Lee YH, Han SH, Hwang SJ (2013) The effects of wavelength and wavelength mixing ratios on microalgae growth and nitrogen, phosphorus removal using Scenedesmus sp. for wastewater treatment. Bioresour Technol 130:75–80CrossRefGoogle Scholar
  11. 11.
    Minhas AK, Hodgson P, Barrow CJ, Adholeya A (2016) A review on the assessment of stress conditions for simultaneous production of microalgal lipids and carotenoids. Front Microbiol 7:546CrossRefGoogle Scholar
  12. 12.
    Huerlimann R, de Nys R, Heimann K (2010) Growth, lipid content, productivity, and fatty acid composition of tropical microalgae for scale-up production. Biotechnol Bioeng 107:245–257CrossRefGoogle Scholar
  13. 13.
    Cao J, Yuan H, Li B, Yang J (2014) Significance evaluation of the effects of environmental factors on the lipid accumulation of Chlorella minutissima UTEX 2341 under low-nutrition heterotrophic condition. Bioresour Technol 152:177–184CrossRefGoogle Scholar
  14. 14.
    Guillard RR, Ryther JH (1962) Studies of marine planktonic diatoms. I. cyclotellanana Hustedt and Detonula confervacea (cleve) Gran. Can J Microbiol 8:229–239CrossRefGoogle Scholar
  15. 15.
    Collos YF, Mornet A, Sciandra N, Waser AL, Harrison PJ (1999) An optical method for the rapid measurement of micromolar concentrations of nitrate in marine phytoplankton cultures. J Appl Phycol 11:179–184CrossRefGoogle Scholar
  16. 16.
    Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol 148:350–382CrossRefGoogle Scholar
  17. 17.
    Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917CrossRefGoogle Scholar
  18. 18.
    Dhup S, Dhawan V (2014) Effect of nitrogen concentration on lipid productivity and fatty acid composition of Monoraphidium sp. Bioresour Technol 152:572–575CrossRefGoogle Scholar
  19. 19.
    Schulze PSC, Barreira LA, Pereira HGC, Perales JA, Varela JCS (2014) Light emitting diodes (LEDs) applied to microalgal production. Trends Biotechnol 32:422–430CrossRefGoogle Scholar
  20. 20.
    Chen CY, Chen YC, Huang HC, Ho SH, Chang JS (2015) Enhancing the production of eicosapentaenoic acid (EPA) from Nannochoropsis oceanica CY2 using innovative photobioreactors with optimal light source arrangements. Bioresour Technol 191:407–413CrossRefGoogle Scholar
  21. 21.
    Yan C, Zheng Z (2014) Performace of mixed LED light wavelengths on biogas upgrade and biogas fluid removal by microalga Chlorella sp. Appl Energy 113:1008–1014CrossRefGoogle Scholar
  22. 22.
    Zhao YG, Wang J, Zhang H, Yan C, Zhang YJ (2013) Effects of various LED light wavelengths and intensities on microalgae-based simultaneous biogas upgrading and digestate nutrient reduction process. Bioresour Technol 136:461–468CrossRefGoogle Scholar
  23. 23.
    Teo CL, Atta M, Bukhari A, Taisir M, Yusuf AM, Idris A (2014) Enhancing growth and lipid production of marine microalgae for biodiesel production via the use of different LED wavelengths. Bioresour Technol 162:38–44CrossRefGoogle Scholar
  24. 24.
    Koller M, Muhr A, Braunegg G (2014) Microalgae as versatile cellular factories for valued products. Algal Res 6:52–63CrossRefGoogle Scholar
  25. 25.
    Lee CG (1999) Calculation of light penetration depth in photobioreactors. Biotechnol Bioprocess Eng 4:78–81CrossRefGoogle Scholar
  26. 26.
    Cheirsilp B, Torpee S (2012) Enhanced growth and lipid production of microalgae under mixotrophic culture condition: effect of light intensity, glucose concentration and fed- batch cultivation. Bioresour Technol 110:510–516CrossRefGoogle Scholar
  27. 27.
    Mujtaba G, Choi WJ, Lee CG, Lee KS (2012) Lipid production by Chlorella vulgaris after a shift from nutrient-rich to nitrogen starvation conditions. Bioresour Technol 123:279–283CrossRefGoogle Scholar
  28. 28.
    Liu Y, Yuan C, Hu G, Li F (2012) Effects of light intensity on the growth and lipid accumulation of microalga Scenedesmus sp. 11–1 under nitrogen limitation. Appl Biochem Biotechnol 166:2127–2137CrossRefGoogle Scholar
  29. 29.
    Ho SH, Chen CNN, Lai YY, Lu WB, Chang JS (2014) Exploring the high lipid production potential of a thermotolerant microalga using statistical optimization and semi-continuous cultivation. Bioresour Technol 163:128–135CrossRefGoogle Scholar
  30. 30.
    Arroussi HE, Benhima R, Bennis I, Mernissi NE, Wahby I (2015) Improvement of the potential of Dunaliella tertiolecta as a source of biodiesel by auxin treatment coupled to salt stress. Renew Energy 77:15–19CrossRefGoogle Scholar
  31. 31.
    Sasi D, Mitra P, Vigueras A, Hill GA (2011) Growth kinetics and lipid production using Chlorella vulgaris in a circulating loop photobioreactor. J Chem Technol Biotechnol 86:875–880CrossRefGoogle Scholar
  32. 32.
    Converti A, Casazza AA, Ortiz EY, Perego P, Borghi MD (2009) Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production. Chem Eng Process 48:1146–1151CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  • Chae Hun Ra
    • 1
  • Phunlap Sirisuk
    • 1
  • Jang-Hyun Jung
    • 2
  • Gwi-Taek Jeong
    • 1
  • Sung-Koo Kim
    • 1
  1. 1.Department of BiotechnologyPukyong National UniversityBusanSouth Korea
  2. 2.Amicogen, lncJinjuSouth Korea

Personalised recommendations