Bioprocess and Biosystems Engineering

, Volume 41, Issue 4, pp 457–465 | Cite as

Effects of light-emitting diode (LED) with a mixture of wavelengths on the growth and lipid content of microalgae

  • Chae Hun Ra
  • Phunlap Sirisuk
  • Jang-Hyun Jung
  • Gwi-Taek Jeong
  • Sung-Koo Kim
Research Paper


Integrations of two-phase culture for cell growth and lipid accumulation using mixed LED and green LED wavelengths were evaluated with the microalgae, Phaeodactylum tricornutum, Isochrysis galbana, Nannochloropsis salina, and Nannochloropsis oceanica. Among the single and mixed LED wavelengths, mixed LED produced higher biomass of the four microalgae, reaching 1.03 g DCW/L I. galbana, followed by 0.95 g DCW/L P. tricornutum, 0.85 g DCW/L N. salina, and 0.62 g DCW/L N. oceanica than single LED or fluorescent lights at day 10. Binary combination of blue and red LEDs could produce the high biomass and photosynthetic pigments in the four microalgae. The highest lipid accumulation during second phase with the exposure to green LED wavelengths was 56.0% for P. tricornutum, 55.2% for I. galbana, 53.0% for N. salina, and 51.0% for N. oceanica. The major fatty acid in the four microalgae was palmitic acid (C16:0) accounting for 38.3–47.3% (w/w) of the total fatty acid content.


Microalgae Fatty acid Mixed LED Green LED Two-phase culture 



This research was a part of the project titled ‘Innovative marine production technology driven by LED-ICT convergence photo-biology (D11514915H480000110)’, funded by the Ministry of Oceans and Fisheries, Korea.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  • Chae Hun Ra
    • 1
  • Phunlap Sirisuk
    • 1
  • Jang-Hyun Jung
    • 2
  • Gwi-Taek Jeong
    • 1
  • Sung-Koo Kim
    • 1
  1. 1.Department of BiotechnologyPukyong National UniversityBusanSouth Korea
  2. 2.Amicogen, lncJinjuSouth Korea

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