Bioprocess and Biosystems Engineering

, Volume 41, Issue 4, pp 449–455 | Cite as

Nitrate removal in a combined bioelectrochemical and sulfur autotrophic denitrification system under high nitrate concentration: effects of pH

  • Dan Chen
  • Dong Wang
  • Zhixing Xiao
  • Hongyu Wang
  • Kai Yang
Research Paper


A combined bioelectrochemical and sulfur autotrophic denitrification (CBSAD) system was established to treat high concentration nitrate under different pH conditions in this study. The microbial communities and structures were evaluated to deeply reveal the nitrate removal mechanisms in this combined system. When initial pH was adjusted to 6.5, the CBSAD system obtained 66.45% denitrification efficiency. The combined system achieved highest nitrate removal efficiency of 96.84% at pH 7.5. However, nitrate removal efficiency decreased to 87.05% when initial pH increased to 8.5. Microbial analyses demonstrated that pH value slightly influenced the bacterial abundances and bacterial species in this CBSAD system under high nitrate concentration condition. Proteobacteria was the most dominant phylum in this system, which accounted for more than 90% of the total phyla. Epsilonproteobacteria, Betaproteobacteria, and Gammaproteobacteria were the most important classes for denitrification process. Genus Sulfurimonas was primarily responsible for high nitrate removal in this CBSAD system.


Autotrophic denitrification Nitrate Microbial communities Abundances 



This work was financially supported by the National Natural Science Foundation of China (NSFC) (51378400) and the National Science and Technology Pillar Program (2014BAL04B04).


  1. 1.
    APHA (2005) Standard methods for the examination of water and wastewater. American Public Health Association (APHA), Washington, DCGoogle Scholar
  2. 2.
    Bhatnagar A, Kumar E, Sillanpaa M (2010) Nitrate removal from water by nano-alumina: characterization and sorption studies. Chem Eng J 163:317–323CrossRefGoogle Scholar
  3. 3.
    Cao S, Du R, Li B, Wang S, Ren N, Peng Y (2017) Nitrite production from partial-denitrification process fed with low carbon/nitrogen (C/N) domestic wastewater: performance, kinetics and microbial community. Chem Eng J 326:1186–1196CrossRefGoogle Scholar
  4. 4.
    Chen C, Xu XJ, Xie P, Yuan Y, Zhou X, Wang AJ, Lee DJ, Ren NQ (2017) Pyrosequencing reveals microbial community dynamics in integrated simultaneous desulfurization and denitrification process at different influent nitrate concentrations. Chemosphere 171:294–301CrossRefGoogle Scholar
  5. 5.
    Chen D, Yang K, Wei L, Wang H (2016) Microbial community and metabolism activity in a bioelectrochemical denitrification system under long-term presence of p-nitrophenol. Bioresour Technol 218:189–195CrossRefGoogle Scholar
  6. 6.
    Chung J, Amin K, Kim S, Yoon S, Kwon K, Bae W (2014) Autotrophic denitrification of nitrate and nitrite using thiosulfate as an electron donor. Water Res 58:169–178CrossRefGoogle Scholar
  7. 7.
    Dasgupta S, Wu S, Goel R (2017) Coupling autotrophic denitrification with partial nitritation-anammox (PNA) for efficient total inorganic nitrogen removal. Bioresour Technol 243:700–707CrossRefGoogle Scholar
  8. 8.
    Huang C, Li ZL, Chen F, Liu Q, Zhao YK, Zhou JZ, Wang AJ (2015) Microbial community structure and function in response to the shift of sulfide/nitrate loading ratio during the denitrifying sulfide removal process. Bioresour Technol 197:227–234CrossRefGoogle Scholar
  9. 9.
    Kong Q, Wang ZB, Niu PF, Miao MS (2016) Greenhouse gas emission and microbial community dynamics during simultaneous nitrification and denitrification process. Bioresour Technol 210:94–100CrossRefGoogle Scholar
  10. 10.
    Liu C, Li W, Li X, Zhao D, Ma B, Wang Y, Liu F, Lee D-J (2017) Nitrite accumulation in continuous-flow partial autotrophic denitrification reactor using sulfide as electron donor. Bioresour Technol 243:1237–1240CrossRefGoogle Scholar
  11. 11.
    Liu H, Yan Q, Shen W (2014) Biohydrogen facilitated denitrification at biocathode in bioelectrochemical system (BES). Bioresour Technol 171:187–192CrossRefGoogle Scholar
  12. 12.
    Mousavi S, Ibrahim S, Aroua MK, Ghafari S (2012) Development of nitrate elimination by autohydrogenotrophic bacteria in bio-electrochemical reactors—a review. Biochem Eng J 67:251–264CrossRefGoogle Scholar
  13. 13.
    Nguyen TNP, Chao S-J, Chen P-C, Huang C (2017) Effects of C/N ratio on nitrate removal and floc morphology of autohydrogenotrophic bacteria in a nitrate-containing wastewater treatment process. J Environ Sci.
  14. 14.
    Su JF, Cheng C, Huang T, Wei L (2017) Performance of the dominant bacterial species and microbial community in autotrophic denitrification coupled with iron cycle in immobilized systems. Mar Pollut Bull 117:88–97CrossRefGoogle Scholar
  15. 15.
    Su JF, Luo XX, Wei L, Ma F, Zheng SC, Shao SC (2016) Performance and microbial communities of Mn(II)-based autotrophic denitrification in a moving bed biofilm reactor (MBBR). Bioresour Technol 211:743–750CrossRefGoogle Scholar
  16. 16.
    Sun H, Wu Q, Yu P, Zhang L, Ye L, Zhang XX, Ren H (2017) Denitrification using excess activated sludge as carbon source: performance and the microbial community dynamics. Bioresour Technol 238:624–632CrossRefGoogle Scholar
  17. 17.
    Wan D, Liu H, Liu R, Qu J (2011) Study of a combined sulfur autotrophic with proton-exchange membrane electrodialytic denitrification technology: sulfate control and pH balance. Bioresour Technol 102:10803–10809CrossRefGoogle Scholar
  18. 18.
    Wan DJ, Liu HJ, Qu JH, Lei PJ, Mao SH, Hou YN (2009) Using the combined bioelectrochemical and sulfur autotrophic denitrification system for groundwater denitrification. Bioresour Technol 100:142–148CrossRefGoogle Scholar
  19. 19.
    Wang H, Qu J (2003) Combined bioelectrochemical and sulfur autotrophic denitrification for drinking water treatment. Water Res 37:3767–3775CrossRefGoogle Scholar
  20. 20.
    Wang Y, Zhang Z, Qiu L, Guo Y, Wang X, Xiong X, Chen S (2015) Effect of temperature downshifts on biological nitrogen removal and community structure of a lab-scale aerobic denitrification process. Biochem Eng J 101:200–208CrossRefGoogle Scholar
  21. 21.
    Xia SQ, Liang J, Xu XY, Shen S (2013) Simultaneous removal of selected oxidized contaminants in groundwater using a continuously stirred hydrogen-based membrane biofilm reactor. J Environ Sci (China) 25:96–104CrossRefGoogle Scholar
  22. 22.
    Xu D, Xiao E, Xu P, Zhou Y, He F, Zhou Q, Xu D, Wu Z (2017) Performance and microbial communities of completely autotrophic denitrification in a bioelectrochemically-assisted constructed wetland system for nitrate removal. Bioresour Technol 228:39–46CrossRefGoogle Scholar
  23. 23.
    Yang W, Lu H, Khanal SK, Zhao Q, Meng L, Chen GH (2016) Granulation of sulfur-oxidizing bacteria for autotrophic denitrification. Water Res 104:507–519CrossRefGoogle Scholar
  24. 24.
    Yi X-H, Wan J, Ma Y, Wang Y (2016) Characteristics and dominant microbial community structure of granular sludge under the simultaneous denitrification and methanogenesis process. Biochem Eng J 107:66–74CrossRefGoogle Scholar
  25. 25.
    Zhou W, Liu X, Dong X, Wang Z, Yuan Y, Wang H, He S (2016) Sulfur-based autotrophic denitrification from the micro-polluted water. J Environ Sci (China) 44:180–188CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  • Dan Chen
    • 1
  • Dong Wang
    • 1
  • Zhixing Xiao
    • 1
  • Hongyu Wang
    • 2
  • Kai Yang
    • 2
  1. 1.College of Urban ConstructionNanjing Tech UniversityNanjingChina
  2. 2.School of Civil EngineeringWuhan UniversityWuhanChina

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